载体基本信息 | |||
载体名称 | pCEP4-Flag-N | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 10209 bp | 载体类型 | Mammalian Expression Vectors |
启动子 | CMV | 载体标签 | N-Flag |
pCEP4-Flag-N 载体载体图谱
pCEP4-Flag-N 载体载体序列
LOCUS Exported 10209 bp ds-DNA circular SYN 13-JAN-2022 DEFINITION synthetic circular DNA ACCESSION . VERSION . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10209) TITLE Direct Submission FEATURES Location/Qualifiers source 1..10209 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 384..587 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 635..658 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /note="FLAG" /translation="DYKDDDDK" polyA_signal 695..829 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin 1552..3341 /note="oriP" /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" promoter 6089..6193 /gene="bla" /note="AmpR promoter" CDS 6194..7054 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7225..7813 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 8244..8389 /note="HSV TK promoter" /note="herpes simplex virus thymidine kinase promoter" CDS 8432..9469 /codon_start=1 /product="hygromycin B phosphotransferase" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE MGEAN" polyA_signal 9511..9559 /note="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)"
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