载体基本信息 | |||
载体名称 | pCAGGS-FLAG-hUNRIP | 载体抗性 | Ampicillin |
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载体长度 | 5819 bp | 载体类型 | Mammalian expression vector |
载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pCAGGS-FLAG-hUNRIP 载体载体图谱
pCAGGS-FLAG-hUNRIP 载体载体序列
LOCUS 40924_8346 5819 bp DNA circular SYN 17-DEC-2018 DEFINITION Mammalian expression vector pCAGGS-FLAG-hUNRIP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5819) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5819) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5819) TITLE Direct Submission REFERENCE 4 (bases 1 to 5819) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5819 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 13..36 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" CDS 43..1092 /gene="STRAP" /label=STRAP /note="Serine-threonine kinase receptor-associated protein from Homo sapiens. Accession#: Q9Y3F4" polyA_signal 1172..1227 /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(1588..1604) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1612..1628) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1636..1666) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1680..1701) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 1759..1955 /label=SV40 promoter /note="SV40 early promoter" polyA_signal 1961..2095 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2333..2921) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3095..3952) /label=AmpR /note="beta-lactamase" promoter complement(3953..4057) /label=AmpR promoter enhancer 4092..4471 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" regulatory 4092..4469 /label=hCMV-IE enhancer /note="hCMV-IE enhancer" /regulatory_class="promoter" promoter 4473..4750 /label=chicken beta-actin promoter intron 4751..5768 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin"
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