载体基本信息 | |||
载体名称 | BPK3079 | 载体抗性 | Ampicillin |
---|---|---|---|
载体长度 | 2225 bp | 载体类型 | Mammalian Expression, CRISPR |
拷贝数 | High Copy | 启动子 | U6 |
克隆方法 | Restriction Enzyme | 5'引物 | OS280 (5'-CAGGGTTATTGTCTCATGAGCGG-3') |
BPK3079 载体载体图谱
BPK3079 载体载体序列
LOCUS Exported 2225 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites). ACCESSION . VERSION . KEYWORDS BPK3079 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2225) AUTHORS Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK TITLE Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. JOURNAL Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. PUBMED 27347757 REFERENCE 2 (bases 1 to 2225) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620." FEATURES Location/Qualifiers source 1..2225 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 82..322 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 82..102 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 253..272 /label=LKO.1 5' /note="Human U6 promoter, forward primer" rep_origin complement(476..1064) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(556..575) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1235..2095) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1858..1877 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2096..2200) /gene="bla" /label=AmpR promoter
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