载体基本信息 | |||
载体名称 | pGAD-mCASP-12 | 载体抗性 | Ampicillin |
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载体长度 | 8908 bp | 载体类型 | Yeast expression vector |
载体来源 | De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. |
pGAD-mCASP-12 载体载体图谱
pGAD-mCASP-12 载体载体序列
LOCUS 40924_20900 8908 bp DNA circular SYN 18-DEC-2018 DEFINITION Yeast expression vector pGAD-mCASP-12, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8908) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 8908) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 8908) TITLE Direct Submission REFERENCE 4 (bases 1 to 8908) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8908 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 5..406 /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 452..472 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS 488..829 /label=GAL4 activation domain /note="activation domain of the GAL4 transcriptional activator" misc_feature complement(847..1780) /label=3UTR /note="3UTR" CDS complement(1784..3040) /gene="Casp12" /label=Casp12 /note="Caspase-12 from Mus musculus. Accession#: O08736" misc_feature complement(3041..3073) /label=5UTR /note="5UTR" terminator 3490..3677 /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(3798..4889) /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" promoter complement(4890..5294) /label=LEU2 promoter primer_bind complement(5336..5352) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5360..5376) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5384..5414) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5429..5450) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5738..6326) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6500..7357) /label=AmpR /note="beta-lactamase" promoter complement(7358..7462) /label=AmpR promoter rep_origin 7744..8908 /label=2u ori /note="yeast 2u plasmid origin of replication"
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