载体基本信息 | |||
载体名称 | pGRB2.1 | 载体抗性 | Ampicillin |
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载体长度 | 5299 bp | 载体类型 | Cloning vector |
载体来源 | Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack BP. |
pGRB2.1 载体载体图谱
pGRB2.1 载体载体序列
LOCUS 40924_22553 5299 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pGRB2.1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5299) AUTHORS Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack BP. TITLE Expression Plasmids for Use in Candida glabrata JOURNAL G3 (Bethesda) 3 (10), 1675-1686 (2013) PUBMED 23934995 REFERENCE 2 (bases 1 to 5299) AUTHORS Zordan RE, Cormack BP. TITLE Direct Submission JOURNAL Submitted (14-MAY-2013) Molecular Biology and Genetics, Johns Hopkins School of Medicine, 725 N Wolfe St., Hunterian 609, Baltimore, MD 21205, USA REFERENCE 3 (bases 1 to 5299) TITLE Direct Submission REFERENCE 4 (bases 1 to 5299) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "G3 (Bethesda)"; date: "2013"; volume: "3"; issue: "10"; pages: "1675-1686" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (14-MAY-2013) Molecular Biology and Genetics, Johns Hopkins School of Medicine, 725 N Wolfe St., Hunterian 609, Baltimore, MD 21205, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5299 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature complement(66..664) /label=Candida glabrata CEN/ARS /note="Candida glabrata CEN/ARS" promoter 666..737 /label=AmpR promoter CDS 738..1595 /label=AmpR /note="beta-lactamase" rep_origin 1769..2357 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2645..2666 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2681..2711 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2719..2735 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2743..2759 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2780..2798 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 2835..2851 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2885..2901) /label=KS primer /note="common sequencing primer, one of multiple similar variants" regulatory 2904..3301 /note="region downstream of HIS3 orf in Candida glabrata BG2" /regulatory_class="terminator" promoter complement(3316..3334) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3344..3360) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 3501..3956 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(4090..4890) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(4891..5106) /label=URA3 promoter
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