- 载体名称:
- pSA-TMbgyg
- 载体抗性:
- Ampicillin
- 载体长度:
- 8570 bp
- 载体类型:
- Signal sequence gene trap vector
- 复制子:
- ori
- 载体来源:
- Shirasawa S, Yoshimi M, Kamochi H, Muneta T, Kimura JH, Hook M, Shinomura T.
- 启动子:
- SP6
pSA-TMbgyg 载体载体图谱
pSA-TMbgyg 载体载体序列
LOCUS 40924_38378 8570 bp DNA circular SYN 18-DEC-2018 DEFINITION Signal sequence gene trap vector pSA-TMbgyg, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8570) AUTHORS Shirasawa S, Yoshimi M, Kamochi H, Muneta T, Kimura JH, Hook M, Shinomura T. TITLE Gene trap screening for cell surface and extracellular matrix molecules produced by chondrocytes JOURNAL J. Biochem. 137 (1), 79-85 (2005) PUBMED 15713886 REFERENCE 2 (bases 1 to 8570) AUTHORS Shinomura T, Shirasawa S. TITLE Direct Submission JOURNAL Submitted (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental University, Tissue Regeneration, Dept. of Hard Tissue Engineering; 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, Fax:81-3-5803-0187) REFERENCE 3 (bases 1 to 8570) TITLE Direct Submission REFERENCE 4 (bases 1 to 8570) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Biochem."; date: "2005"; volume: "137"; issue: "1"; pages: "79-85" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental University, Tissue Regeneration, Dept. of Hard Tissue Engineering"; volume: " 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, Fax"; pages: "81-3-5803-0187" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8570 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 17..120 /label=splicing acceptor /note="splicing acceptor" misc_feature 144..804 /gene="TM(CD4)" /product="transmembrane domain of rat CD4" gene 144..804 /gene="TM(CD4)" /label=TM(CD4) CDS 814..3855 /label=lacZ /note="beta-galactosidase" CDS 3871..4905 /label=HygR /note="hygromycin B phosphotransferase" polyA_signal 4950..4998 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" regulatory 5189..5408 /regulatory_class="terminator" promoter complement(5439..5457) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5475..5491) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5499..5515) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5523..5553) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5568..5589) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5877..6465) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6639..7496) /label=AmpR /note="beta-lactamase" promoter complement(7497..7601) /label=AmpR promoter rep_origin 7935..8390 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 8531..8547 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 8554..8570 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase"
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