LOCUS       40924_1014       11450 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector Ganesh-R1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Swanson CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     A directional recombination cloning system for restriction- and 
            ligation-free construction of GFP, DsRed, and lacZ transgenic 
            Drosophila reporters
  JOURNAL   Gene 408 (1-2), 180-186 (2008)
  PUBMED    18077106
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Rogers CI, Hinrichs T, Johnson LA, Zhao Y, Barolo S.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 11450)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11450)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 408 
            (1-2), 180-186 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2007) Cell "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..233)
                     /label=P element 3' end
                     /note="P element 3' end"
     gene            242..4247
                     /gene="white"
                     /allele="+mC"
                     /label=white
     protein_bind    4255..4379
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4404..4434
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4488..5144
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             5489..5791
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5835..5959)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5965..6712
                     /label=spacer sequence includes partial KanR gene
                     /note="spacer sequence includes partial KanR gene"
     regulatory      6713..6846
                     /label=Drosophila melanogaster Hsp70 minimal promoter
                     /note="Drosophila melanogaster Hsp70 minimal promoter"
                     /regulatory_class="promoter"
     CDS             6920..7594
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     misc_feature    7598..7714
                     /gene="DsRed.T4-NLS-SV40t"
                     /label=Drosophila melanogaster tra gene NLS
                     /note="Drosophila melanogaster tra gene NLS"
     polyA_signal    7852..7986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(8105..8690)
                     /label=P element 5' end
     rep_origin      complement(8925..9513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9687..10544)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10545..10649)
                     /label=AmpR promoter
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
       61 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
      121 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
      181 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
      241 gctgcagcaa ttctagtatg tatgtaagtt aataaaaccc atttttgcgg aaagtagata
      301 aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg atcagtttta
      361 aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc tctcactcaa
      421 aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc
      481 cgtctctttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg ttacctagcg
      541 tcaatgtccg ccttcagttg cactttgtca gcggtttcgt gacgaagctc caagcggttt
      601 acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt atttttgttt
      661 gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg aaagtgtgaa
      721 aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc agcaaacacc
      781 catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta agttcacttg
      841 atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca
      901 aaaatcccaa accgcaatcg cacaaacaaa tagtgacacg aaacagatta ttctggtagc
      961 tgtgctcgct atataagaca atttttaaga tcatatcatg atcaagacat ctaaaggcat
     1021 tcattttcga ctacattctt ttttacaaaa aatataacaa ccagatattt taagctatgc
     1081 acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta
     1141 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
     1201 acgcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc
     1261 tgccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca
     1321 cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc
     1381 ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca
     1441 ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc
     1501 ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga
     1561 acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc
     1621 ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca
     1681 acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt
     1741 tatcggctcc ctaacggcca gggaacacct gattttccaa gccatggtgc ggatgccacg
     1801 acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct
     1861 cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg
     1921 agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg
     1981 cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa
     2041 gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct
     2101 gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac
     2161 tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct
     2221 agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca
     2281 atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc
     2341 gtgatcggat cgccaagata tggcgacaat tttgccatta gcaaagtagc ccgggatatg
     2401 gagcagttgt tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac
     2461 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
     2521 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
     2581 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat
     2641 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
     2701 ttgcagaaga taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt
     2761 ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc
     2821 aagtgggtgt gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc
     2881 aaaacgtctt tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat
     2941 ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt
     3001 atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt
     3061 gccgaattgc cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg
     3121 atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg
     3181 gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg
     3241 gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg
     3301 aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac
     3361 gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc
     3421 acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc
     3481 tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg
     3541 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
     3601 cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac tgtttattgc
     3661 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     3721 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     3781 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
     3841 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
     3901 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
     3961 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc
     4021 aaatggttcc gagtggttca tttcgtctca atagaaatta gtaataaata tttgtatgta
     4081 caatttattt gctccaatat atttgtatat atttccctca cagctatatt tattctaatt
     4141 taatattatg actttttaag gtaatttttt gtgacctgtt cggagtgatt agcgttacaa
     4201 tttgaactga aagtgacatc cagtgtttgt tccttgtgta gatgcatgca tcaaacaagt
     4261 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     4321 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     4381 cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga
     4441 ttttgagtta ggatccggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     4501 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     4561 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     4621 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     4681 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     4741 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     4801 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     4861 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     4921 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     4981 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     5041 tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc
     5101 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaatct agaggatccg
     5161 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     5221 tatatactga tatgtatacc cgaagtatgt caaaaagagg tgtgctatga agcagcgtat
     5281 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     5341 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     5401 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     5461 tttgctgacg agaacaggga ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     5521 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     5581 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta
     5641 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     5701 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     5761 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     5821 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     5881 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     5941 ctttcttgta caaagtggtt cgatggcgcg ccctcatcga gcatcaaatg aaactgcaat
     6001 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     6061 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     6121 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     6181 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     6241 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     6301 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     6361 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     6421 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt tccggggatc
     6481 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     6541 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     6601 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caagcgatag
     6661 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccacc gcggagcgcc
     6721 ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg
     6781 aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat
     6841 ctgcagccaa gctgatcctc tagggtacgc gctagagtcg agctagcagg cctgtttaaa
     6901 cgatccaccg gtcgccacca tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt
     6961 caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     7021 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     7081 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
     7141 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
     7201 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     7261 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
     7321 cgtaatgcag aagaagacta tgggctggga gccctccacc gagcgcctgt acccccgcga
     7381 cggcgtgctg aagggcgaga tccacaaggc cctgaagctg aaggacggcg gccactacct
     7441 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
     7501 cgtggactcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
     7561 cgagcgcgcc gagggccgcc accacctgtt cctgtacaag agcaggcaca gaaggcatcg
     7621 ccagcgctct aggagccgca atcgcagccg aagtcgcagc agtgaacgaa aacgccgtca
     7681 acggagccga agtcgcagca gtgaacgaag acgctacttg tacaagtaaa gcggccgcga
     7741 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     7801 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     7861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagtc
     7981 tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact
     8041 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctagaattaa
     8101 ttcggctgct gctctaaacg acgcatttcg tactccaaag tacgaatttt ttccctcaag
     8161 ctcttatttt cattaaacaa tgaacaggac ctaacgcaca gtcacgttat tgtttacata
     8221 aatgattttt tttactattc aaacttactc tgtttgtgta ctcccactgg tatagccttc
     8281 ttttatcttt tctggttcag gctctatcac tttactaggt acggcatctg cgttgagtcg
     8341 cctcctttta aatgtctgac cttttgcagg tgcagccttc cactgcgaat cattaaagtg
     8401 ggtatcacaa atttgggagt tttcaccaag gctgcaccca aggctctgct cccacaattt
     8461 tctcttaata gcacacttcg gcacgtgaat taattttact ccagtcacag ctttgcagca
     8521 aaatttgcaa tatttcattt ttttttattc cacgtaaggg ttaatgtttt caaaaaaaaa
     8581 ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt aagtgtatac
     8641 ttcggtaagc ttcggctttc gacgggacca ccttatgtta tttcatcatg ggccagaccc
     8701 acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat gaccttgccc
     8761 gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc cacgtccgcc
     8821 cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta cgacaaccat
     8881 ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca taggctccgc
     8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9121 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9961 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10681 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10741 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    10801 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    10861 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    10921 gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg acagtcgctc
    10981 caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac agcgaaagag
    11041 caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga tttgaaaagt
    11101 atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt ttgatgtaag
    11161 gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact tgtggggatg
    11221 ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg cacaaatata
    11281 attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata atggtaaaaa
    11341 aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg cccgaagtct
    11401 tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
//
