LOCUS Exported 10540 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Expression vector LIC-pKLAC-LC1, complete sequence. ACCESSION JF327848 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10540) AUTHORS Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B. TITLE High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection JOURNAL PLoS ONE 6 (4), E18900 (2011) PUBMED 21541323 REFERENCE 2 (bases 1 to 10540) AUTHORS Dortay H, Mueller-Roeber B. TITLE Direct Submission JOURNAL Submitted (14-FEB-2011) Molecular Biology, University of Potsdam, Germany, Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 14476, Germany REFERENCE 3 (bases 1 to 10540) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..10540 /organism="Expression vector LIC-pKLAC-LC1" /mol_type="other DNA" /label=Kluyveromyces lactis expression vector /note="Kluyveromyces lactis expression vector" /db_xref="taxon:1029639" misc_feature 14..16 /label=start codon /note="start codon" misc_feature 26..43 /label=6xHis tag /note="6xHis tag" CDS 26..43 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" misc_feature 53..1036 /label=infrared fluorescent protein /note="infrared fluorescent protein" CDS 53..1036 /codon_start=1 /product="bacteriophytochrome-based monomeric infrared fluorescent protein (Shu et al., 2009)" /label=IFP1.4 /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLR LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEAGMDELYK" misc_feature 1037..1057 /label=TEV recognition site /note="TEV recognition site" CDS 1037..1057 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" misc_feature 1076..1083 /label=PmeI recognition site /note="PmeI recognition site" misc_feature 1084..1750 /label=LIC stuffer fragment /note="LIC stuffer fragment" misc_feature 1751..1758 /label=PmeI recognition site /note="PmeI recognition site" terminator 1804..2422 /label=LAC4 terminator /note="transcription terminator for Kluyveromyces lactis LAC4" regulatory 1804..2386 /regulatory_class="terminator" /note="terminator" CDS 3146..4792 /codon_start=1 /product="acetamidase" /label=acetamidase /protein_id="AEE81072.1" /translation="MPQSWEELAADKRARLAKTIPDEWKVQTLPAEDSVIDFPKKSGIL SEAELKITEASAADLVSKLAAGELTSVEVTLAFCKRAAIAQQLTNCAHEFFPDAALAQA RELDEYYAKHKRPVGPLHGLPISLKDQLRVKGYETSMGYISWLNKYDEGDSVLTTMLRK AGAVFYVKTSVPQTLMVCETVNNIIGRTVNPRNKNWSCGGSSGGEGAIVGIRGGVIGVG TDIGGSIRVPAAFNFLYGLRPSHGRLPYAKMANSMEGQETVHSVVGPITHSVEDLRLFT KSVLGQEPWKYDSKVIPMPWRQSESDIIASKIKNGGLNIGYYNFDGNVLPHPPILRGVE TTVAALAKAGHTVTPWTPYKHDFGHDLISHIYAADGSADVMRDISASGEPAIPNIKDLL NPNIKAVNMNELWDTHLQKWNYQMEYLEKWREAEEKAGKELDAIIAPITPTAAVRHDQF RYYGYASVINLLDFTSVVVPVTFADKNIDKKNESFKAVSELDALVQEEYDPEAYHGAPV AVQVIGRRLSEERTLAIAEEVGKLLGNVVTP" promoter 5428..6084 /label=LAC4 promoter (5') /note="5' portion of the Kluyveromyces lactis LAC4 promoter" regulatory 5501..6081 /regulatory_class="promoter" /note="5' promoter region" promoter complement(6101..6119) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6133..6149) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 6157..6173 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6181..6211) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6226..6247 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin 6535..7123 /label=pMB1 origin /note="pMB1 origin" CDS complement(7294..8154) /codon_start=1 /product="beta-lactamase" /label=beta-lactamase /protein_id="AEE81073.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8155..8259) /gene="bla" /label=AmpR promoter rep_origin 8286..8741 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 8882..8898 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 8908..10540 /regulatory_class="promoter" /note="3' promoter region" promoter 8912..10440 /label=LAC4 promoter (3') /note="3' portion of a modified Kluyveromyces lactis LAC4 promoter" protein_bind 10446..10462 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 aagcttgaaa aaaatgggct ccggccatca ccatcaccat cacctcgaat caatggctcg 61 ggaccctctg ccattctttc cacctctgta cctgggcggc cctgagatta caaccgagaa 121 ctgcgagaga gagcctatcc acattcctgg gtccatccag ccacacgggg ctctgctcac 181 agctgacggc cactccggag aggtgctcca agtgtccctg aatgccgcta ccttcctggg 241 ccaggagcct actgtgctgc gggggcagac cctggctgcc ctgctccccg agcagtggcc 301 agccctgcag gcagccctgc ccccaggatg tccagatgcc ctccaataca gggccaccct 361 cgactggcca gctgctgggc acctcagcct gactgtgcat cgggtggctg aactcctgat 421 cctggagttc gaacctaccg aggcctggga cagcattggc cctcacgccc tgaggaacgc 481 catgtttgct ctggaaagcg ctccaaacct gcgggctctg gccgaagtcg caacacaaac 541 tgtgagagaa ctgactggct tcgatcgggt gatgctgtac aaatttgccc ctgacgccac 601 tggagagatg attgctgagg ccagacggga gggcatgcag gcttttctgg gccacaggtt 661 tcccgcatcc cacacccctg cacaagctag ggccctctac acaagacacc tgctccggct 721 gaccgcagac accagggctg cagcagtgcc cctcgacccc gtgctgaatc cccagacaaa 781 tgctcctaca cctctgggcg gagctgtcct cagagctaca tccccaatgc acatgcagta 841 cctgaggaat atgggagtgg gctcctccct gagcgtcagc gtcgtggtcg gcggccagct 901 gtggggactg attgtctgcc accatcagac accctacgtg ctgccaccag atctgcggac 961 caccctggag gagctgggga gaaagctgtc cgggcaggtg cagaggaaag aagccggaat 1021 ggacgagctg tataaagaga acctgtattt tcagggcggc ggcatgggtt cttctgttta 1081 aacctcagtg tcatcattcg aaagattcga aatatttccc aaagaaagct catggcccaa 1141 ccacaacaca aacaaaggag taacggcagc atgctcccat gcgggggaaa gcagttttta 1201 cagaaatttg ctatggctga cggagaagga gggctcatac ccaaagctga aaaattctta 1261 tgtgaacaag agagggaaag aagtccttgt actgtggggt attcatcacc cgtctaacag 1321 taaggagcaa cagaatatct atcagaatga aaatgcttat gtctctgtag tgacttcaaa 1381 ttataacagg agatttaccc cggaaatagc agaaagaccc aaagtaagag atcaagctgg 1441 gaggatgaac tattactgga ccttgctaaa acccggagac acaataatat ttgaggcaaa 1501 tggaaatcta atagcaccaa ggtatgcttt cgcactgagt agaggctttg ggtccggcat 1561 catcacctca aacgcatcaa tgcatgagtg taacacgaag tgtcaaacac ccctgggagc 1621 tataaacagc agtctccctt tccagaatat acacccagtc acaataggag agtgcccaaa 1681 atacgtcagg agtgccaaat tgaggatggt tacaggacta aggaacattc cgtccattca 1741 atccgctagc gtttaaacag ggcgagaacc tgcggccgct taattaaggc cttgaatcga 1801 gaatttatac ttagataagt atgtacttac aggtatattt ctatgagata ctgatgtata 1861 catgcatgat aatatttaaa cggttattag tgccgattgt cttgtgcgat aatgacgttc 1921 ctatcaaagc aatacactta ccacctatta catgggccaa gaaaatattt tcgaacttgt 1981 ttagaatatt agcacagagt atatgatgat atccgttaga ttatgcatga ttcattccta 2041 caactttttc gtagcataag gattaattac ttggatgcca ataaaaaaaa aaaacatcga 2101 gaaaatttca gcatgctcag aaacaattgc agtgtatcaa agtaaaaaaa agattttcac 2161 tacatgttcc ttttgaagaa agaaaatcat ggaacattag atttacaaaa atttaaccac 2221 cgctgattaa cgattagacc gttaagcgca caacaggtta ttagtacaga gaaagcattc 2281 tgtggtgttg ccccggactt tcttttgcga cataggtaaa tcgaatacca tcatactatc 2341 ttttccaatg actccctaaa gaaagactct tcttcgatgt tgtatacgtt ggagcatagg 2401 gcaagaattg tggcttgaga tcatcctttt gttgtttccg ggtgtacaat atggacttcc 2461 tcttttctgg caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct 2521 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg 2581 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata 2641 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt 2701 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct 2761 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag 2821 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg 2881 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag 2941 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc 3001 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct 3061 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca 3121 atcaaggaat tccggatccg ccaccatgcc tcaatcctgg gaagaactgg ccgctgataa 3181 gcgcgcccgc ctcgcaaaaa ccatccctga tgaatggaaa gtccagacgc tgcctgcgga 3241 agacagcgtt attgatttcc caaagaaatc ggggatcctt tcagaggccg aactgaagat 3301 cacagaggct tccgctgcgg atcttgtgtc caagctggcg gccggagagt tgacctcggt 3361 ggaagttacg ctagcattct gtaaacgggc agcaatcgcc cagcagttaa caaactgcgc 3421 ccacgagttc ttccctgacg ccgctctcgc gcaggcaagg gaactcgatg aatactacgc 3481 aaagcacaag agacccgttg gtccactcca tggcctcccc atctctctca aagaccagct 3541 tcgagtcaag ggctacgaaa catcaatggg ctacatctca tggctaaaca agtacgacga 3601 aggggactcg gttctgacaa ccatgctccg caaagccggt gccgtcttct acgtcaagac 3661 ctctgtcccg cagaccctga tggtctgcga gacagtcaac aacatcatcg ggcgcaccgt 3721 caacccacgc aacaagaact ggtcgtgcgg cggcagttct ggtggtgagg gtgcgatcgt 3781 tgggattcgt ggtggcgtca tcggtgtagg aacggatatc ggtggctcga ttcgagtgcc 3841 ggccgcgttc aacttcctgt acggtctaag gccgagtcat gggcggctgc cgtatgcaaa 3901 gatggcgaac agcatggagg gtcaggagac ggtgcacagc gttgtcgggc cgattacgca 3961 ctctgttgag gacctccgcc tcttcaccaa atccgtcctc ggtcaggagc catggaaata 4021 cgactccaag gtcatcccca tgccctggcg ccagtccgag tcggacatta ttgcctccaa 4081 gatcaagaac ggcgggctca atatcggcta ctacaacttc gacggcaatg tccttccaca 4141 ccctcctatc ctgcgcggcg tggaaactac cgtcgccgca ctcgccaaag ccggtcacac 4201 cgtgaccccg tggacgccat acaagcacga tttcggccac gatctcatct cccatatcta 4261 cgcggctgac ggcagcgccg acgtaatgcg cgatatcagt gcatccggcg agccggcgat 4321 tccaaatatc aaagacctac tgaacccgaa catcaaagct gttaacatga acgagctctg 4381 ggacacgcat ctccagaagt ggaattacca gatggagtac cttgagaaat ggcgggaggc 4441 tgaagaaaag gccgggaagg aactggacgc catcatcgcg ccgattacgc ctaccgctgc 4501 ggtacggcat gaccagttcc ggtactatgg gtatgcctct gtgatcaacc tgctggattt 4561 cacgagcgtg gttgttccgg ttacctttgc ggataagaac atcgataaga agaatgagag 4621 tttcaaggcg gttagtgagc ttgatgccct cgtgcaggaa gagtatgatc cggaggcgta 4681 ccatggggca ccggttgcag tgcaggttat cggacggaga ctcagtgaag agaggacgtt 4741 ggcgattgca gaggaagtgg ggaagttgct gggaaatgtg gtgactccat agcccggggg 4801 gggctcgatc ccctcgcgag ttggttcagc tgctgcctga ggctggacga cctcgcggag 4861 ttctaccggc agtgcaaatc cgtcggcatc caggaaacca gcagcggcta tccgcgcatc 4921 catgcccccg aactgcagga gtggggaggc acgatggccg ctttggtcga tctagattac 4981 gtggaagaaa ggtagtaaaa gtagtagtat aagtagtaaa aagaggtaaa aagagaaaac 5041 cggctacata ctagagaagc acgtacacaa aaactcatag gcacttcatc atacgacagt 5101 ttcttgatgc attataatag tgtattagat attttcagaa atatgcatag aacctcctct 5161 tgcctttact ttttatacat agaacattgg cagatttact tacactactt tgtttctacg 5221 ccatttcttt tgttttcaac acttagacaa gttgttgaga accggactac taaaaagcaa 5281 tgttcccact gaaaatcatg tacctgcagg ataataaccc cctaattctg catcgatcca 5341 gtatgttttt ttttctctac tcatttttac ctgaagatag agcttctaaa acaaaaaaaa 5401 tcagtgatta catgcatatt gtgtgttcta gtaaccaaag gaaaggaaca gatagataaa 5461 attccgagac tgtcaaatta ggtttttttc tttttttttg gcgggagtca gtgggccgaa 5521 atatgttctt ggcctagaac ttaatctggt ttgatcatgc caatacttgc ctgagtgccc 5581 gactttttgc ccaccctctt gccttctgtc atccttcaaa acccacctgt tttccagccg 5641 tatcttcgct cgcatctaca catactgtgc catatcttgt gtgtagccgg acgtgactat 5701 gaccaaaaac aaacaaggag aactgttcgc cgatttgtaa cactcctgca tccatccaag 5761 tgggtatgcg ctatgcaatg ttaagctagg tcaggtcaga ccaggtccaa ggacagcaac 5821 ttgactgtat gcaaccttta ccatctttgc acagaacata cttgtagcta gctagttaca 5881 cttatggacc gaaaaggcac cccaccatgt ctgtccggct ttagagtacg gccgcagacc 5941 gctgatttgc cttgccaagc agtagtcaca atgcatcgca tgagcacacg ggcacgggca 6001 cgggcacagg aaccattggc aaaaatacca gatacactat accgacgtat atcaagccca 6061 agtttaaaat tcctaaattt ccgcggctac ttttcaattc cctatagtga gtcgtattaa 6121 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 6181 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6241 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6301 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6541 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6961 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7261 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7321 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7381 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7441 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7501 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7561 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 7621 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 7681 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7741 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7801 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7861 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 7921 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 7981 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8041 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8101 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8161 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8221 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8281 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 8341 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 8401 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 8461 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 8521 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 8581 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 8641 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 8701 atttaacgcg aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg 8761 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 8821 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 8881 tgtaaaacga cggccagtgc caagctcccg cggggatcga ctcataaaat agtaaccttc 8941 taatgcgtat ctattgacta ccaaccatta gtgtggttgc agaaggcgga attctccctt 9001 cttcgaattc agcttgcttt ttcatttttt attttccatt tttcagtttt tgtttgtgtc 9061 gaatttagcc agttgcttct ccaagatgaa aaaaacccct gcgcagtttc tgtgctgcaa 9121 gatcctaatc gacttttcca ccccccacaa aagtaaatgt tcttttgtta cattcgcgtg 9181 ggtagctagc tccccgaatc ttcaaaggac ttagggactg cactacatca gagtgtgttc 9241 acctggtttg ctgcctggtt tgaaagaaaa gagcagggaa ctcgcgggtt cccggcgaat 9301 aatcatgcga tagtcctttg gccttccaag tcgcatgtag agtagacaac agacagggag 9361 ggcaggaagg atctttcact gagatcctgt atcttgttgg gtaagtcgga tgaaagggga 9421 atcgtatgag attggagagg atgcggaaga ggtaacgcct tttgttaact tgtttaatta 9481 ttatggggca ggcgagaggg ggaggaatgt atgtgtgtga ggcgggcgag acggagccat 9541 ccaggccagg tagaaataga gaaagccgaa tgttagacaa tatggcagcg tagtagagta 9601 ggtaggtagg caagtactgc tagcaaagag gagaagggta agctcactct tcgcattcca 9661 caccgttagt gtgtcagttt gaacaaaaaa acaatcatca taccaattga tggactgtgg 9721 actggctttt ggaacggctt ttcggactgc gattattcgt gaggaatcaa ggtaggaatt 9781 tggtcatatt tacggacaac agtgggtgat tcccatatgg agtaggaaaa cgagatcatg 9841 gtatcctcag atatgttgcg gaattctgtt caccgcaaag ttcagggtgc tctggtgggt 9901 ttcggttggt ctttgctttg cttctccctt gtcttgcatg ttaataatag cctagcctgt 9961 gagccgaaac ttagggtagg cttagtgttg gaacgtacat atgtatcacg ttgacttggt 10021 ttaaccaggc gacctggtag ccagccatac ccacacacgt tttttgtatc ttcagtatag 10081 ttgtgaaaag tgtagcggaa atttgtggtc cgagcaacag cgtctttttc tagtagtgcg 10141 gtcggttact tggttgacat tggtatttgg actttgttgc tacaccattc actacttgaa 10201 gtcgagtgtg aagggtatga tttctagtgg tgaacacctt tagttacgta atgttttcat 10261 tgctgtttta cttgagattt cgattgagaa aaaggtattt aatagctcga atcaatgtga 10321 gaacagagag aagatgttct tccctaactc gaaaggtata tgaggcttgt gtttcttagg 10381 agaattatta ttcttttgtt atgttgcgct tgtagttgga aaaggtgaag agacaaaagc 10441 tggaattgtg agcggataac aagctcaaca cttgaaattt aggaaagagc agaatttggc 10501 aaaaaaaata aaaaaaaaat aaacacacat actcatcgag //