LOCUS       .                      11443 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        26..44
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             358..4176
                     /gene="S"
                     /label="S"
                     /note="Spike glycoprotein from Severe acute respiratory
                     syndrome coronavirus 2. Accession#: P0DTC2"
     CDS             4483..5088
                     /label="LexA"
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     CDS             5107..5340
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     terminator      5345..5591
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(5610..5628)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5795..6250
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6550..6954
                     /label="LEU2 promoter"
     CDS             6967..8058
                     /label="LEU2"
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     CDS             complement(8782..9594)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      10036..10624
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(10801..11302)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 ccgcggatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
       61 tcatttggcg agcgttggtt ggtggatcaa gcccacgcgt aggcaatcct cgagcagatc
      121 cgccaggcgt gtatatatag cgtggatggc caggcaactt tagtgctgac acatacaggc
      181 atatatatat gtgtgcgacg acacatgatc atatggcatg catgtgctct gtatgtatat
      241 aaaactcttg ttttcttctt ttctctaaat attctttcct tatacattag gacctttgca
      301 gcataaatta ctatacttct atagacacgc aaacacaaat acacacacta atctagaatg
      361 ttcgtgttcc tcgtgctgct cccactggtg tctagccagt gcgtgaacct caccacccgc
      421 acccagctcc ctcctgccta cacaaacagc ttcacaaggg gcgtgtacta ccctgacaag
      481 gtgttccggt ctagcgtgct gcactctaca caggacctgt tcctcccttt cttctctaac
      541 gtgacatggt tccacgccat ccatgtatcc ggcacaaacg gcacaaagcg gttcgacaac
      601 cccgtgctcc ctttcaacga cggcgtgtac ttcgccagca ccgagaagtc taacattatt
      661 agaggctgga ttttcggcac cacactcgac tctaagaccc agtctctcct gattgtgaac
      721 aacgccacaa acgtggtgat taaggtgtgc gagttccagt tctgcaacga ccctttcctg
      781 ggcgtgtact accacaagaa caacaagtct tggatggagt ccgagttccg ggtgtactct
      841 agcgccaaca actgcacatt cgagtacgtg tctcagcctt tcctcatgga cctggagggc
      901 aagcagggca acttcaagaa cctgagagag ttcgtgttca agaacattga cggctacttc
      961 aagatttact ccaagcacac cccaattaac ctggtgaggg acctccctca gggcttctcc
     1021 gccctggaac ccctcgtgga cctcccaatt ggcattaaca tcacccggtt ccagacactg
     1081 ctcgccctcc accggtctta cctgacccca ggcgactctt ctagcggctg gacagccggc
     1141 gccgccgcct actacgtggg ctacctccag ccacgcacat tcctcctgaa gtacaacgag
     1201 aacggcacaa ttaccgacgc cgtggactgc gccctcgacc cactgagcga gacaaagtgc
     1261 acactgaagt ctttcacagt ggagaagggc atttaccaga catctaactt ccgggtgcag
     1321 cctaccgagt ctattgtgcg gttcccaaac attaccaacc tgtgcccttt cggcgaggtg
     1381 ttcaacgcca cccgcttcgc ctctgtgtac gcctggaacc ggaagcggat ttctaactgc
     1441 gtggccgact actctgtgct gtacaacagc gcctctttct ccacattcaa gtgctacggc
     1501 gtgtccccta caaagctgaa cgacctgtgc ttcacaaacg tgtacgccga ctctttcgtg
     1561 atccggggcg acgaggtgag gcagattgcc ccaggccaga caggcaagat tgccgactac
     1621 aactacaagc tccctgacga cttcacaggc tgcgtgatcg cctggaactc taacaacctc
     1681 gactctaagg tgggcggcaa ctacaactac ctgtacagac tgttccgcaa gtctaacctg
     1741 aagcctttcg agcgggacat tagcaccgag atttaccagg ccggcagcac cccatgcaac
     1801 ggcgtggagg gcttcaactg ctacttccca ctccagtctt acggcttcca gcctacaaac
     1861 ggcgtgggct accagccata ccgggtggtg gtgctgtctt ttgaattgct ccacgccccc
     1921 gccacagtgt gcggccctaa gaagtccaca aacctggtga agaacaagtg cgtgaacttc
     1981 aacttcaacg gcctcacagg cacaggcgtg ctcaccgagt ccaacaagaa gttcctgcct
     2041 ttccagcagt ttggccggga cattgccgac accaccgacg ccgtgaggga ccctcagaca
     2101 ttggagattc tggacatcac cccatgcagc ttcggcggcg tgtctgtgat cacccccggc
     2161 accaacacat ctaaccaggt ggccgtgctg taccaggacg tgaactgcac agaggtgcca
     2221 gtggccatcc acgccgacca gctgacccct acatggaggg tgtactctac aggctctaac
     2281 gtgttccaga ctcgcgccgg ctgcctgatt ggcgccgagc acgtaaacaa cagctacgag
     2341 tgcgacatcc caattggcgc cggcatttgc gcctcttacc agacccagac aaactctcct
     2401 agacgggccc ggtctgtggc ctctcagtct attattgcct acaccatgtc cctgggcgcc
     2461 gagaactctg tggcctactc taacaactct attgccatcc ctacaaactt cacaatttct
     2521 gtgaccaccg agattctccc agtgtctatg acaaagacat ccgtggactg caccatgtac
     2581 atttgcggcg actctaccga gtgctctaac ctcctgctcc agtacggctc tttctgcacc
     2641 cagctcaacc gggccctcac aggcattgcc gtggagcagg acaagaacac acaggaggtg
     2701 ttcgcccagg tgaagcagat ttacaagacc cctcctatta aggacttcgg cggcttcaac
     2761 ttctctcaga ttctccccga ccctagcaag ccatctaagc ggtccttcat tgaggacctg
     2821 ctgttcaaca aggtgacact ggccgacgcc ggcttcatta agcagtacgg cgactgcctg
     2881 ggcgacatcg ccgctcgtga cctgatttgc gcccagaagt tcaacggcct gacagtgctc
     2941 cccccactgc tcaccgacga gatgattgcc cagtacacca gcgccctcct cgccggcacc
     3001 atcacatctg gctggacctt cggcgccggc gccgccctac agatcccttt cgccatgcag
     3061 atggcctacc gcttcaacgg catcggcgtg acccagaacg tgctgtacga gaaccagaag
     3121 ctcatcgcca accagttcaa ctctgccatt ggcaagattc aggactctct gtctagcaca
     3181 gccagcgccc tgggcaaact gcaagacgtg gtgaaccaga acgcccaggc cctcaacaca
     3241 ctggtgaagc aactctcgtc taacttcggc gccattagct ctgtgctgaa cgacattctg
     3301 tcccgactcg acaaggtgga ggccgaggtg cagattgaca gactgattac aggcagactc
     3361 caatctctac agacatacgt gacacagcag ctcattagag ccgccgagat cagagcctcc
     3421 gccaacctgg ccgccacaaa gatgagcgag tgcgtgctgg gccagtccaa gagagtggac
     3481 ttctgcggca agggctacca cctcatgtct ttccctcagt ccgcccctca cggcgtggtg
     3541 ttcctccatg ttacatacgt gccagcccag gagaagaact tcaccacagc ccccgccatt
     3601 tgccacgacg gcaaggccca cttccctaga gagggcgtgt tcgtgtccaa cggcacccac
     3661 tggttcgtga cccagagaaa cttctacgag cctcagatta tcaccaccga caacaccttc
     3721 gtgtctggca actgcgacgt ggtgattggc attgtgaaca acacagtgta cgacccattg
     3781 caacccgaac ttgactcttt caaggaggaa cttgacaagt acttcaagaa ccacacatct
     3841 cccgacgtgg acctgggcga cattagcggc attaacgcct ctgtggtgaa cattcagaag
     3901 gagatcgaca ggctgaacga ggtggccaag aacctgaacg agtctctgat tgacttgcaa
     3961 gagctgggca agtacgagca gtacattaag tggccttggt acatttggct gggcttcatc
     4021 gccggcctga tcgccattgt gatggtgacc atcatgctgt gctgcatgac atcttgctgc
     4081 tcttgcctga agggctgctg ctcttgcggc tcttgctgca agttcgacga ggacgactct
     4141 gagcccgtgc tgaagggcgt gaagctccac tacacatata tgatgctttt gcaagctttc
     4201 cttttccttt tggctggttt tgcagccaaa atatctgcaa tggccattac ggccaggcct
     4261 ttaattaagg ccgcctcggc catctgcagg aattcgatat caagcttatc gataccgtcg
     4321 accatgtcgg gggggatccc tccagatcaa caaagattga tctttgccgg taagcagcta
     4381 gaagacggta gaacgctgtc tgattacaac attcagaagg agtccacctt acatcttgtg
     4441 ctaaggctaa gaggtggtat gcacagatca gcttgcggcc gcatgaaagc gttaacggcc
     4501 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     4561 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     4621 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatc
     4681 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     4741 ccacttctgg cgcaacagca tattgaaggt cattatcagg tcgacccttc cttattcaag
     4801 ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga tgaaagatat cggcattatg
     4861 gatggtgact tgctggcagt gcataaaact caggatgtac gtaacggtca ggtcgttgtc
     4921 gcacgtattg atgacgaggt taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa
     4981 ctgttgccag aaaatagcga gtttaaacca attgtcgtag atcttcgtca gcagagcttc
     5041 accattgaag ggctggcggt tggggttatt cgcaacggcg actggctgga attcccgggg
     5101 atctgggccc ccccgaccga tgtcagcctg ggggacgagc tccacttaga cggcgaggac
     5161 gtggcgatgg cgcatgccga cgcgctagac gatttcgatc tggacatgtt gggggacggg
     5221 gattccccgg gtccgggatt taccccccac gactccgccc cctacggcgc tctggatatg
     5281 gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg
     5341 taggtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     5401 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     5461 atgttagtat taagaacgtt atttatattt caaatttttc tttttttctg tacagacgcg
     5521 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg
     5581 ctttaatttg cggccggtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     5641 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5701 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5761 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     5821 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5881 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5941 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6001 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6061 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6121 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6181 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6241 tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     6301 tatcgacggt cgaggagaac ttctagtata tccacatacc taatattatt gccttattaa
     6361 aaatggaatc ccaacaatta catcaaaatc cacattctct tcaaaatcaa ttgtcctgta
     6421 cttccttgtt catgtgtgtt caaaaacgtt atatttatag gataattata ctctatttct
     6481 caacaagtaa ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag aaatatcttg
     6541 accgcagtta actgtgggaa tactcaggta tcgtaagatg caagagttcg aatctcttag
     6601 caaccattat ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc
     6661 aaattcgatg actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt
     6721 tcaactgaaa aattgggaga aaaaggaaag gtgagaggcc ggaaccggct tttcatatag
     6781 aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg acaatattat
     6841 ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc
     6901 ttacctttta catttcagca atatatatat atatttcaag gatataccat tctaatgtct
     6961 gcccctatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa
     7021 atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag
     7081 ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg tgtcccactt
     7141 ccagatgagg cgctggaagc ctccaagaag gttgatgccg ttttgttagg tgctgtgggt
     7201 ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa
     7261 gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac
     7321 ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta
     7381 gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat
     7441 agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc
     7501 ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct
     7561 tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag
     7621 gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta
     7681 aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt
     7741 atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac
     7801 accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag
     7861 gttgacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg
     7921 cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga
     7981 actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa
     8041 gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt
     8101 ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt
     8161 agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatagt
     8221 aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga aaaagaattg
     8281 cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag gtgtaacaga
     8341 aaatcatgaa actacgattc ctaatttgat attggaggat tttctctaaa aaaaaaaaaa
     8401 tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt
     8461 cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg
     8521 ccttacgacg tagtcgatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8581 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8641 ggcatccgct tacagacaag ctgtgaccgt ctccgggacc acatgtggat gatccagttc
     8701 gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa
     8761 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     8821 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     8881 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     8941 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     9001 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     9061 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     9121 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     9181 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     9241 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     9301 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     9361 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     9421 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     9481 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     9541 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc
     9601 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc
     9661 ttgtgcaatg taacatcaga gattttgaga cacaacgtgg cttactagga tcgatccgac
     9721 atgtggcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9841 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9961 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10021 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10081 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10141 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    10201 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10261 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10321 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10381 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10441 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10501 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10561 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10621 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10681 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10741 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10801 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
    10861 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag
    10921 gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa
    10981 aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag atatacattc
    11041 gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac
    11101 aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat
    11161 ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt
    11221 aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat
    11281 aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg gaaatgtgcg
    11341 cggaacccct aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    11401 attcattaat gcagctggca cgacaggttt cccgactgga aag
//
