LOCUS       V040826                11670 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040826
VERSION     V040826
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2221..2432
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     intron          2433..3371
                     /note="intron upstream of the start codon of human EF-1α"
                     /label="EF-1α intron A"
     regulatory      3387..3396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3396..3425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3432..6347
                     /label="Naa25(NM_172722)"
                     /note="Naa25(NM_172722)"
                     /gene="Naa25"
     misc_feature    6464..7013
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             7016..7615
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7639..8227
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8434..9067
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9195..9211)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9219..9235
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9243..9273)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9288..9309
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9597..10185)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10356..11216)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11217..11321)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    11369..11503
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca ccatggaaca aaaactcatc tcagaagagg
     3421 atctgacgcg tatggcgacg cggggccatg tgcaggaccc taacgaccgg cgcctccggc
     3481 ccatttacga ttatcttgat aatggaaata ataaaatggc aattcagcaa gctgataaac
     3541 tgttgaagaa acacaaagat cttcactgtg caaaggttct caaggccatt ggtttacaga
     3601 gaactggcaa gcaggaagaa gctttcacct tggctcagga ggtggctgcc cttgagccca
     3661 cagatgacaa ctccctgcag gccctgacga ttctctaccg agagatgcac cggccggagt
     3721 tagtcacaaa gctctatgag gcggctgtga agaaagttcc caacagcgag gagtaccact
     3781 ctcacctctt catggcctat gccagagtgg gcgagtacaa gaagatgcag caggctggca
     3841 tggctctgta taagattgtc cccaaaaacc cttactactt ttggtcagtg atgagcttga
     3901 tcatgcaatc tatatccgca cgggatgaga acctctccaa aaccatgttt ctgcccctgg
     3961 cggagagaat ggtagagaag atggtgaagg aggacaagat agaggctgaa gctgaggttg
     4021 aactttatta catgattctg gagcgcttgg gaaagtacca ggaagccctg gatgtcatta
     4081 gaggaaaatt aggagagaag ttgacaagtg aaattcagag tcgggaaaac aaatgcatgg
     4141 ccatgtacaa gaagctgagc aagtggccgg agtgcaacgc cctttcccga cgcctccttc
     4201 tgaaaaactc agatgactgg cagttctatc tgacttattt cgattctgtc tttcgactga
     4261 ttgaggaagc ctggacgccg cctgctgagg gggaacactc cttagaaggg gaagtacact
     4321 gctctgcaga agatgctgtg aagtttatag aggacaggat aacagaggca tcacaaagct
     4381 ctcgacatgt ccgaggccca cacctagcta agctggagct catcaggcgc ttgcggagcc
     4441 aaggctgtaa tgacgaatac aagctgggtg accctgaaga gttaatgttc cagtatttca
     4501 agaagttcgg ggataagcca tgctgcttca cagacctgaa ggtgttcgtt gacctcttgc
     4561 ctgccgccca gtgcacacaa ttcattaacc agctccttgg agttgttcca ttgtcgacgc
     4621 cgacagagga taagcttgcc ctgccggccg acatccgagg cctgcagcag catctgtgtg
     4681 ttgtgcagct gacacggctc cttggcttgt accacagcat ggataagagt cagaagctgg
     4741 atgtggtcaa agaactgatg ttaaggtacc agcacggact ggagtttggg cgctcttgtt
     4801 tgaagacaga gttgcagttc tcagactatt actgcctgct cgctgttcat gtgctcattg
     4861 atgtatggag agaagcaggt gaggagactg cggtctggca ggccctgact ctgctggaag
     4921 aaggcttaac ccacagccca tccaatgctc agttcaaatt gctacttgtt cggatctact
     4981 gcgtgctggg tgcatttgaa ccagtggtgg atctctactc cagtctcgat gctaagcaca
     5041 tccagcacga caccattggc tatcttctga cccgatatgc tgcatccctg ggtcagtatg
     5101 ctgctgcctc ccagtcctgt aactttgcac tcaggttttt ccactccaac cagaaagata
     5161 cctcagaata cattattcaa gcttacaaat atggtgcatt tgagaagatc ccagagttta
     5221 tcgcttttag gaacaggctg aataattctc tccattttgc acaagtacgc actgagcgga
     5281 tgctgttaga ccttctactt gaagcaaaca tatcaattag cttggcagaa agtatcaaat
     5341 caatgaatct gaggccagag gaagatgatg ttccatggga agatttgcga gacaacagag
     5401 acttggatgt tttcttcagt tgggatccaa aagacaggaa tgtttctgaa gagcataaga
     5461 aactctcctt ggaggaagaa accatgtggt taagaattcg ctccttaacg ttgaggctga
     5521 tcagtggact tccaagtctt acccaccctg tggagcccaa gaactcagag aagatgtcag
     5581 agaacggtgt gtcctcccgg atcgacatcc ttcgtttgct cctccaacag ctagaggtgg
     5641 ccgtggagac ggggaagcgg tttattgaga aggaaattca gtaccccttc cttgggcctg
     5701 ttcccaccag gatgggcagg ttcttcagtt ctggctgctg tcagtgccaa gtccagtcct
     5761 tccatctggt cagcgacatg tatgagcttg acaccagtgg cttagagggt acagtggata
     5821 tacaggaacg aatagaaaat agtcttgcat ctttactaga actattgaaa ggtgtcttca
     5881 gcacatgtaa aggggacctc ttagaagtta cagatggcaa tgtgaagacc cagcctgcag
     5941 tcctagagaa tctggtcttc ttcgttgaga ctatttctgt catcctttgg gtgtccagtt
     6001 actgtgaaag tgtcttgcga ccatacaaat taaatatcca gaaaaagaaa aagaagaaaa
     6061 aagaaacgag catcatcatg cctccaatct tcaccagttt ccaagactac gttactggac
     6121 ttcagactgt aatttccaat gctgtggatc atattaaagg acttgaagca catctgattg
     6181 cacttagact tgaggagctc acgttagagg aaacttctat ctctacggaa gaaagaaaat
     6241 tttcaaagac tgtgcagggg aaagtccaga gcagttacct gcactcactt ctggagacag
     6301 gggagctgct gagaaaaaga cttgagacta caaagaaact aaaaatttaa gaattcctcg
     6361 agggcggccg ctctagagtc gacgggccgc ggtaacaatt gttaactaac ttaagctagc
     6421 aacggtttcc ctctagcggg atcaattccg cccccccccc ctaacgttac tggccgaagc
     6481 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     6541 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     6601 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     6661 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     6721 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     6781 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     6841 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     6901 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     6961 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatgac
     7021 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7081 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7141 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7201 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7261 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7321 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7381 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7441 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7501 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7561 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacccgg
     7621 gcggggcgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7681 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7741 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7801 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7861 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7921 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7981 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8041 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8101 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8161 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8221 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
     8281 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     8341 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     8401 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
     8461 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     8521 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     8581 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     8641 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     8701 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     8761 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     8821 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     8881 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     8941 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9001 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9061 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9121 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
     9181 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9241 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9301 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9481 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9541 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9601 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9661 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9721 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9781 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9841 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9901 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9961 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10021 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10081 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10141 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10201 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10261 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10321 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10381 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10441 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10501 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10561 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10621 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10681 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10741 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10801 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10861 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10921 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10981 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11041 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11101 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11161 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11221 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11281 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11341 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    11401 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11461 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    11521 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    11581 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    11641 ttgttaaata tgtactacaa acttagtagt
//
