LOCUS       V042087                12366 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042087
VERSION     V042087
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    681..806
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1721..1765
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    2028..2143
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     promoter        2221..2432
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     intron          2433..3371
                     /note="intron upstream of the start codon of human EF-1α"
                     /label="EF-1α intron A"
     regulatory      3387..3396
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             3396..3425
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3432..7043
                     /label="Xpo5(NM_028198)"
                     /note="Xpo5(NM_028198)"
                     /gene="Xpo5"
     misc_feature    7160..7709
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /label="IRES"
     CDS             7712..8311
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8335..8923
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9130..9763
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     primer_bind     complement(9891..9907)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9915..9931
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9939..9969)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9984..10005
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10293..10881)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11052..11912)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11913..12017)
                     /gene="bla"
                     /label="AmpR promoter"
     polyA_signal    12065..12199
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt
     2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3361 ttccatttca ggtgtcgtga ggatccgcca ccatggaaca aaaactcatc tcagaagagg
     3421 atctgacgcg tatggagatg gagcaagtga acgcgctgtg cgaggagcta gtgaaagcgg
     3481 tgacggtcat gatggacccc agctctaccc agcgctaccg gctggaagcc ctcaagtttt
     3541 gtgaagagtt taaagaaaaa tgccctatct gtgttccatg tggcttgaag ttggctgaaa
     3601 aaacacaaat tgccatcgtc agacattttg gccttcagat cctggagcat gttgtcaagt
     3661 ttcgatggaa cagcatgtcc cgattggaga aggtctatct gaagaacagt gtcatggagc
     3721 tgatcgcaaa tggaacactg aggattttgg aggaggagaa ccacattaaa gatgttctgt
     3781 ctagaattgt ggtagagatg atcaaacgag agtggccaca gcactggcct gacatgctca
     3841 tggagttgga tactctcttc aggcaagggg aaacgcagag ggagttggtg atgttcatcc
     3901 ttctccgact ggccgaggat gtagtgacct ttcagacgct ccccactcaa agaagaaggg
     3961 atattcagca aacattgacg cagaacatgg aaagaatctt gaattttcta ctcaatacgc
     4021 ttcaggaaaa tgtaaacaag taccaacaaa tgaagacaga ttcatctcag gaggcagagg
     4081 ctcaagccaa ctgtcgagta agcgtggcgg ccctgaacac tctagccggc tacattgact
     4141 gggtatcttt gaaccatatc actgccgaga actgtaaact cgtggagact ctgtgcctgc
     4201 ttctcaatga gcaggagctg cagttgggag ccgccgagtg tctgctcatt gcagtcagca
     4261 gaaaaggcaa gctggaggac cggaagcgct tgatgatcct gtttggagat gtcgccatgc
     4321 attatattct ctcggccgca cagactgcag atggaggagg cttggtcgaa aaacactacc
     4381 tcttcctgaa gagactttgt caggtgttat gtgcactggg aaatctgcta tgtgcactgc
     4441 tggccttaga tgctaacata caaacaccta taaactttgg aatgtacctg gaatcttttc
     4501 ttgctttcac aacccatcca agtcagtttt tacgctcttc aacccatatg acgtggggag
     4561 ccctcttcag gcatgaagtt ctgtcccgtg atccggcact gttagcagta ataccaaaat
     4621 atcttcgtgc ttctatgaca aacttggtca agatgggctt tccttctaaa acagatagcc
     4681 ccagctgtga atattcccga tttgatttcg atagtgatga ggacttcaat gctttcttca
     4741 actcctcccg agcccagcat ggagaggtgg tgaggtgtgt gtgtcgactg gatcctaaga
     4801 ctagcttcca gatggcggca gaatggctca agtatcagct gtcagcttct attgacactg
     4861 gacctgtgaa ctcatgttct acagctggaa ctggagaggg aggcttctgc tccatcttct
     4921 caccttcata tgtgcagtgg gaagctatga cttttttttt ggaaagtgtg atcaaccaga
     4981 tgtttcgaac actagacaaa gaagagctcc cggttagtga tgggatagag ctgctgcagc
     5041 tggtgttgaa ctttgaaatc aaggatcccc tcgtcctgtc ctgcgtcctc actaatgtct
     5101 cagcgctctt cccatttgtc acatacaagc ctgcgttctt gccccaggtc ttctctaagc
     5161 tcttttcatt tgttactttt gaatctgttg gggaaagtaa ggcccccagg actcgggcag
     5221 tcaggaatgt aaggagacat gcttgttcct ccatcaacaa gatgtgtcgg gactacccag
     5281 accttgttct gcccaatttt gacatgcttt atagccacgt gaagcaactc ctctccaatg
     5341 agctcctcct gacccagatg gagaagtgcg ccctcatgga agctctggtt ctcgttagca
     5401 accaattcaa ggattatgag cggcagaagt tattcctaga ggagctgatg gcaccggtgg
     5461 tcaacatctg gctttctgaa gaaatgtgca gagcgctgtc agatattgac tccttcattg
     5521 cctatgtggg tgctgatctg aaaagctgtg acccagctgt ggaggatcca tgtggcttga
     5581 accgtgcacg aatgagcttt tgtgtgtaca gcatcctggg ggttatgaga cgcactagct
     5641 ggccttccga cctagaagaa gccaaagctg ggggctttgt ggtgggctac acacccagtg
     5701 gaaatcccat cttccgtaac ccctgcacag agcagatcct cagacttctc gacaatttgc
     5761 ttgcccttgt aagaactcac aatactttat atacaccgga aatgctaacg aaaatggcag
     5821 aacctttcac caaggctctg gatatagttg aatctgaaaa aacggcaata ttaggattac
     5881 ctcagcctct cttggaattc aacgaccacc ctgtctatag aaccactctg gagaggatgc
     5941 agcggttctt tggcattctc tatgaaaact gttaccatat cctagggaag gcaggccctt
     6001 ccatgcagca agatttctac actgtggagg accttgcttc ccagctcctt ggatcagctt
     6061 tcgtcaactt gaacaatatt cctgacttcc ggcttagatc tatgcttcgt gtcttcgtga
     6121 agcccctcgt gctcttctgc ccctcagaac actatgaaac cctgatatct cccatccttg
     6181 gacctctctt cacctacctc cacatgaggc tctctcagaa gtggcatgtc atcaaccaga
     6241 ggagcatcct gtgtggagaa gatgagattg cagaggacaa ccccgagtct caggaaatgc
     6301 tcgaggaaca gctggtgagg atgctcaccc gagaagccat ggacctaatc atggcttgct
     6361 gtgtgtcgaa gaagactgcc gaccacacag ccgctcccac tgcagatgga gatgatgaag
     6421 agatgatggc cactgaagta gccccctcgt ctgtggtgga gctcacagac ctgggcaaat
     6481 gcctcatgaa gcacgaggat gtctgcacag cactgctaat cacagcattt aattctctga
     6541 cctggaagga cacactgtct tgccagaggg ctaccacaca gctctgctgg cccctcctca
     6601 aacaggtgat gtctgggacc ctgctcgcag acgctgtcac ttggcttttc accagtgtgc
     6661 tgaaaggact gcagatgcac gggcagcacg atgggtgcat ggcttccctg gtccacttgg
     6721 ccttccagat atacgaggcg ctgcgcccca ggtacctaga gataagagca gtaatggagc
     6781 agatccctga aataaacaag gagtctctgg accaatttga ctgcaagctt ttaaacccct
     6841 cccttcaaaa agcagctgat aaacgacgga aggaccactt caaacgtcta atcgctggct
     6901 gcattgggaa acccttggga gaacagttcc gaaaagaagt tcacattaag aaccttccct
     6961 ggcttttcaa aaaacccaaa ccaatgttgg agacagaagt gctggacagt gaggagggtg
     7021 gactggccac catctttgaa ccctgagaat tcctcgaggg cggccgctct agagtcgacg
     7081 ggccgcggta acaattgtta actaacttaa gctagcaacg gtttccctct agcgggatca
     7141 attccgcccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     7201 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     7261 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     7321 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     7381 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     7441 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     7501 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     7561 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     7621 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     7681 gtggttttcc tttgaaaaac acgataatac catgaccgag tacaagccca cggtgcgcct
     7741 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     7801 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     7861 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     7921 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     7981 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     8041 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     8101 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     8161 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     8221 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     8281 ctggtgcatg acccgcaagc ccggtgcctg acccgggcgg ggcgcgtctg gaacaatcaa
     8341 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8401 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8461 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8521 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8581 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8641 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8701 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     8761 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8821 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8881 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     8941 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     9001 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     9061 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     9121 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     9181 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     9241 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     9301 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     9361 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     9421 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     9481 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     9541 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     9601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     9781 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     9841 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9901 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9961 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    10021 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    10081 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    10141 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    10201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    10261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    11041 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    11101 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    11161 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    11221 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    11281 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    11341 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    11401 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    11461 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    11521 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    11581 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    11641 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    11701 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11761 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11821 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11881 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11941 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    12001 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    12061 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12121 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12181 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    12241 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    12301 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    12361 agtagt
//
