LOCUS       V032027                10833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032027
VERSION     V032027
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     728..744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        1442..1645
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1734..6125
                     /label="GRIN2A(NM_000833)"
                     /note="GRIN2A(NM_000833)"
                     /gene="GRIN2A"
     promoter        6170..6381
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6394..6662
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6697..7296
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    7404..7525
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8812..8828)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8836..8852
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8860..8890)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8905..8926
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9214..9802)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9973..10833)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatgggca
     1741 gagtgggcta ttggaccctg ctggtgctgc cggcccttct ggtctggcgc ggtccggcgc
     1801 cgagcgcggc ggcggagaag ggtccccccg cgctaaatat tgcggtgatg ctgggtcaca
     1861 gccacgacgt gacagagcgc gaacttcgaa cactgtgggg ccccgagcag gcggcggggc
     1921 tgcccctgga cgtgaacgtg gtagctctgc tgatgaaccg caccgacccc aagagcctca
     1981 tcacgcacgt gtgcgacctc atgtccgggg cacgcatcca cggcctcgtg tttggggacg
     2041 acacggacca ggaggccgta gcccagatgc tggattttat ctcctcccac accttcgtcc
     2101 ccatcttggg cattcatggg ggcgcatcta tgatcatggc tgacaaggat ccgacgtcta
     2161 ccttcttcca gtttggagcg tccatccagc agcaagccac ggtcatgctg aagatcatgc
     2221 aggattatga ctggcatgtc ttctccctgg tgaccactat cttccctggc tacagggaat
     2281 tcatcagctt cgtcaagacc acagtggaca acagctttgt gggctgggac atgcagaatg
     2341 tgatcacact ggacacttcc tttgaggatg caaagacaca agtccagctg aagaagatcc
     2401 actcttctgt catcttgctc tactgttcca aagacgaggc tgttctcatt ctgagtgagg
     2461 cccgctccct tggcctcacc gggtatgatt tcttctggat tgtccccagc ttggtctctg
     2521 ggaacacgga gctcatccca aaagagtttc catcgggact catttctgtc tcctacgatg
     2581 actgggacta cagcctggag gcgagagtga gggacggcat tggcatccta accaccgctg
     2641 catcttctat gctggagaag ttctcctaca tccccgaggc caaggccagc tgctacgggc
     2701 agatggagag gccagaggtc ccgatgcaca ccttgcaccc atttatggtc aatgttacat
     2761 gggatggcaa agacttatcc ttcactgagg aaggctacca ggtgcacccc aggctggtgg
     2821 tgattgtgct gaacaaagac cgggaatggg aaaaggtggg caagtgggag aaccatacgc
     2881 tgagcctgag gcacgccgtg tggcccaggt acaagtcctt ctccgactgt gagccggatg
     2941 acaaccatct cagcatcgtc accctggagg aggccccatt cgtcatcgtg gaagacatag
     3001 accccctgac cgagacgtgt gtgaggaaca ccgtgccatg tcggaagttc gtcaaaatca
     3061 acaattcaac caatgagggg atgaatgtga agaaatgctg caaggggttc tgcattgata
     3121 ttctgaagaa gctttccaga actgtgaagt ttacttacga cctctatctg gtgaccaatg
     3181 ggaagcatgg caagaaagtt aacaatgtgt ggaatggaat gatcggtgaa gtggtctatc
     3241 aacgggcagt catggcagtt ggctcgctca ccatcaatga ggaacgttct gaagtggtgg
     3301 acttctctgt gccctttgtg gaaacgggaa tcagtgtcat ggtttcaaga agtaatggca
     3361 ccgtctcacc ttctgctttt ctagaaccat tcagcgcctc tgtctgggtg atgatgtttg
     3421 tgatgctgct cattgtttct gccatagctg tttttgtctt tgaatacttc agccctgttg
     3481 gatacaacag aaacttagcc aaagggaaag caccccatgg gccttctttt acaattggaa
     3541 aagctatatg gcttctttgg ggcctggtgt tcaataactc cgtgcctgtc cagaatccta
     3601 aagggaccac cagcaagatc atggtatctg tatgggcctt cttcgctgtc atattcctgg
     3661 ctagctacac agccaatctg gctgccttca tgatccaaga ggaatttgtg gaccaagtga
     3721 ccggcctcag tgacaaaaag tttcagagac ctcatgacta ttccccacct tttcgatttg
     3781 ggacagtgcc taatggaagc acggagagaa acattcggaa taactatccc tacatgcatc
     3841 agtacatgac caaatttaat cagaaaggag tagaggacgc cttggtcagc ctgaaaacgg
     3901 ggaagctgga cgctttcatc tacgatgccg cagtcttgaa ttacaaggct gggagggatg
     3961 aaggctgcaa gctggtgacc atcgggagtg ggtacatctt tgccaccacc ggttatggaa
     4021 ttgcccttca gaaaggctct ccttggaaga ggcagatcga cctggccttg cttcagtttg
     4081 tgggtgatgg tgagatggag gagctggaga ccctgtggct cactgggatc tgccacaacg
     4141 agaagaacga ggtgatgagc agccagctgg acattgacaa catggcgggc gtattctaca
     4201 tgctggctgc cgccatggcc cttagcctca tcaccttcat ctgggagcac ctcttctact
     4261 ggaagctgcg cttctgtttc acgggcgtgt gctccgaccg gcctgggttg ctcttctcca
     4321 tcagcagggg catctacagc tgcattcatg gagtgcacat tgaagaaaag aagaagtctc
     4381 cagacttcaa tctgacggga tcccagagca acatgttaaa actcctccgg tcagccaaaa
     4441 acatttccag catgtccaac atgaactcct caagaatgga ctcacccaaa agagctgctg
     4501 acttcatcca aagaggttcc ctcatcatgg acatggtttc agataagggg aatttgatgt
     4561 actcagacaa caggtccttt caggggaaag agagcatttt tggagacaac atgaacgaac
     4621 tccaaacatt tgtggccaac cggcagaagg ataacctcaa taactatgta ttccagggac
     4681 aacatcctct tactctcaat gagtccaacc ctaacacggt ggaggtggcc gtgagcacag
     4741 aatccaaagc gaactctaga ccccggcagc tgtggaagaa atccgtggat tccatacgcc
     4801 aggattcact atcccagaat ccagtctccc agagggatga ggcaacagca gagaatagga
     4861 cccactccct aaagagccct aggtatcttc cagaagagat ggcccactct gacatttcag
     4921 aaacgtcaaa tcgggccacg tgccacaggg aacctgacaa cagtaagaac cacaaaacca
     4981 aggacaactt taaaaggtca gtggcctcca aataccccaa ggactgtagt gaggtcgagc
     5041 gcacctacct gaaaaccaaa tcaagctccc ctagagacaa gatctacact atagatggtg
     5101 agaaggagcc tggtttccac ttagatccac cccagtttgt tgaaaatgtg accctgcccg
     5161 agaacgtgga cttcccggac ccctaccagg atcccagtga aaacttccgc aagggggact
     5221 ccacgctgcc aatgaaccgg aaccccttgc ataatgaaga ggggctttcc aacaacgacc
     5281 agtataaact ctactccaag cacttcacct tgaaagacaa gggttccccg cacagtgaga
     5341 ccagcgagcg ataccggcag aactccacgc actgcagaag ctgcctttcc aacatgccca
     5401 cctattcagg ccacttcacc atgaggtccc ccttcaagtg cgatgcctgc ctgcggatgg
     5461 ggaacctcta tgacatcgat gaagaccaga tgcttcagga gacaggtaac ccagccaccg
     5521 gggagcaggt ctaccagcag gactgggcac agaacaatgc ccttcaatta caaaagaaca
     5581 agctaaggat tagccgtcag cattcctacg ataacattgt cgacaaacct agggagctag
     5641 accttagcag gccctcccgg agcataagcc tcaaggacag ggaacggctt ctggagggaa
     5701 atttttacgg cagcctgttt agtgtcccct caagcaaact ctcggggaaa aaaagctccc
     5761 ttttccccca aggtctggag gacagcaaga ggagcaagtc tctcttgcca gaccacacct
     5821 ccgataaccc tttcctccac tcccacaggg atgaccaacg cttggttatt gggagatgcc
     5881 cctcggaccc ttacaaacac tcgttgccat cccaggcggt gaatgacagc tatcttcggt
     5941 cgtccttgag gtcaacggca tcgtactgtt ccagggacag tcggggccac aatgatgtgt
     6001 atatttcgga gcatgttatg ccttatgctg caaataagaa taatatgtac tctaccccca
     6061 gggttttaaa ttcctgcagc aatagacgcg tgtacaagaa aatgcctagt atcgaatctg
     6121 atgtttaagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     6181 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     6241 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     6301 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     6361 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     6421 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     6481 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     6541 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     6601 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     6661 tccaagctgt gaccggcgcc tacgctagac gccaccatga ccgagtacaa gcccacggtg
     6721 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6781 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6841 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6901 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6961 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7021 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7081 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7141 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7201 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7261 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     7321 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7381 gctttaatgc ctttgtatca gttaacttgt ttattgcagc ttataatggt tacaaataaa
     7441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7501 tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga tgtcaattag
     7561 tctatcagaa gctatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa
     7621 acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg
     7681 tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg
     7741 gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca
     7801 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     7861 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     7921 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     7981 atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat
     8041 aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc
     8101 ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca
     8161 aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg
     8221 agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca
     8281 agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg
     8341 atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca
     8401 tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg
     8461 ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc
     8521 gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata
     8581 ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca
     8641 acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg
     8701 gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat
     8761 ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct
     8821 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8881 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8941 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9001 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9061 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9121 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9181 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9241 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9301 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9361 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9421 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9481 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9541 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9601 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     9661 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9721 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9781 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9841 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9901 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9961 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10021 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10081 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10141 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10201 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10261 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10321 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10381 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10441 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10501 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10561 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10621 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10681 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10741 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10801 aataagggcg acacggaaat gttgaatact cat
//
