LOCUS       V020108                11425 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020108
VERSION     V020108
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(1..105)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     728..744
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        1442..1645
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             1734..5903
                     /label="MET(NM_000245)"
                     /note="MET(NM_000245)"
                     /gene="MET"
     promoter        5952..6163
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6176..6444
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6473..6482
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6503..7165
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7235..7288
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7289..7888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    7996..8117
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9404..9420)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9428..9444
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9452..9482)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9497..9518
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9806..10394)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10565..11425)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
     1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
     1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
     1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
     1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
     1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1621 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgcc accatgaagg
     1741 cccccgctgt gcttgcacct ggcatcctcg tgctcctgtt taccttggtg cagaggagca
     1801 atggggagtg taaagaggca ctagcaaagt ccgagatgaa tgtgaatatg aagtatcagc
     1861 ttcccaactt caccgcggaa acacccatcc agaatgtcat tctacatgag catcacattt
     1921 tccttggtgc cactaactac atttatgttt taaatgagga agaccttcag aaggttgctg
     1981 agtacaagac tgggcctgtg ctggaacacc cagattgttt cccatgtcag gactgcagca
     2041 gcaaagccaa tttatcagga ggtgtttgga aagataacat caacatggct ctagttgtcg
     2101 acacctacta tgatgatcaa ctcattagct gtggcagcgt caacagaggg acctgccagc
     2161 gacatgtctt tccccacaat catactgctg acatacagtc ggaggttcac tgcatattct
     2221 ccccacagat agaagagccc agccagtgtc ctgactgtgt ggtgagcgcc ctgggagcca
     2281 aagtcctttc atctgtaaag gaccggttca tcaacttctt tgtaggcaat accataaatt
     2341 cttcttattt cccagatcat ccattgcatt cgatatcagt gagaaggcta aaggaaacga
     2401 aagatggttt tatgtttttg acggaccagt cctacattga tgttttacct gagttcagag
     2461 attcttaccc cattaagtat gtccatgcct ttgaaagcaa caattttatt tacttcttga
     2521 cggtccaaag ggaaactcta gatgctcaga cttttcacac aagaataatc aggttctgtt
     2581 ccataaactc tggattgcat tcctacatgg aaatgcctct ggagtgtatt ctcacagaaa
     2641 agagaaaaaa gagatccaca aagaaggaag tgtttaatat acttcaggct gcgtatgtca
     2701 gcaagcctgg ggcccagctt gctagacaaa taggagccag cctgaatgat gacattcttt
     2761 tcggggtgtt cgcacaaagc aagccagatt ctgccgaacc aatggatcga tctgccatgt
     2821 gtgcattccc tatcaaatat gtcaacgact tcttcaacaa gatcgtcaac aaaaacaatg
     2881 tgagatgtct ccagcatttt tacggaccca atcatgagca ctgctttaat aggacacttc
     2941 tgagaaattc atcaggctgt gaagcgcgcc gtgatgaata tcgaacagag tttaccacag
     3001 ctttgcagcg cgttgactta ttcatgggtc aattcagcga agtcctctta acatctatat
     3061 ccaccttcat taaaggagac ctcaccatag ctaatcttgg gacatcagag ggtcgcttca
     3121 tgcaggttgt ggtttctcga tcaggaccat caacccctca tgtgaatttt ctcctggact
     3181 cccatccagt gtctccagaa gtgattgtgg agcatacatt aaaccaaaat ggctacacac
     3241 tggttatcac tgggaagaag atcacgaaga tcccattgaa tggcttgggc tgcagacatt
     3301 tccagtcctg cagtcaatgc ctctctgccc caccctttgt tcagtgtggc tggtgccacg
     3361 acaaatgtgt gcgatcggag gaatgcctga gcgggacatg gactcaacag atctgtctgc
     3421 ctgcaatcta caaggttttc ccaaatagtg caccccttga aggagggaca aggctgacca
     3481 tatgtggctg ggactttgga tttcggagga ataataaatt tgatttaaag aaaactagag
     3541 ttctccttgg aaatgagagc tgcaccttga ctttaagtga gagcacgatg aatacattga
     3601 aatgcacagt tggtcctgcc atgaataagc atttcaatat gtccataatt atttcaaatg
     3661 gccacgggac aacacaatac agtacattct cctatgtgga tcctgtaata acaagtattt
     3721 cgccgaaata cggtcctatg gctggtggca ctttacttac tttaactgga aattacctaa
     3781 acagtgggaa ttctagacac atttcaattg gtggaaaaac atgtacttta aaaagtgtgt
     3841 caaacagtat tcttgaatgt tataccccag cccaaaccat ttcaactgag tttgctgtta
     3901 aattgaaaat tgacttagcc aaccgagaga caagcatctt cagttaccgt gaagatccca
     3961 ttgtctatga aattcatcca accaaatctt ttattagtgg tgggagcaca ataacaggtg
     4021 ttgggaaaaa cctgaattca gttagtgtcc cgagaatggt cataaatgtg catgaagcag
     4081 gaaggaactt tacagtggca tgtcaacatc gctctaattc agagataatc tgttgtacca
     4141 ctccttccct gcaacagctg aatctgcaac tccccctgaa aaccaaagcc tttttcatgt
     4201 tagatgggat cctttccaaa tactttgatc tcatttatgt acataatcct gtgtttaagc
     4261 cttttgaaaa gccagtgatg atctcaatgg gcaatgaaaa tgtactggaa attaagggaa
     4321 atgatattga ccctgaagca gttaaaggtg aagtgttaaa agttggaaat aagagctgtg
     4381 agaatataca cttacattct gaagccgttt tatgcacggt ccccaatgac ctgctgaaat
     4441 tgaacagcga gctaaatata gagtggaagc aagcaatttc ttcaaccgtc cttggaaaag
     4501 taatagttca accagatcag aatttcacag gattgattgc tggtgttgtc tcaatatcaa
     4561 cagcactgtt attactactt gggtttttcc tgtggctgaa aaagagaaag caaattaaag
     4621 atctgggcag tgaattagtt cgctacgatg caagagtaca cactcctcat ttggataggc
     4681 ttgtaagtgc ccgaagtgta agcccaacta cagaaatggt ttcaaatgaa tctgtagact
     4741 accgagctac ttttccagaa gatcagtttc ctaattcatc tcagaacggt tcatgccgac
     4801 aagtgcagta tcctctgaca gacatgtccc ccatcctaac tagtggggac tctgatatat
     4861 ccagtccatt actgcaaaat actgtccaca ttgacctcag tgctctaaat ccagagctgg
     4921 tccaggcagt gcagcatgta gtgattgggc ccagtagcct gattgtgcat ttcaatgaag
     4981 tcataggaag agggcatttt ggttgtgtat atcatgggac tttgttggac aatgatggca
     5041 agaaaattca ctgtgctgtg aaatccttga acagaatcac tgacatagga gaagtttccc
     5101 aatttctgac cgagggaatc atcatgaaag attttagtca tcccaatgtc ctctcgctcc
     5161 tgggaatctg cctgcgaagt gaagggtctc cgctggtggt cctaccatac atgaaacatg
     5221 gagatcttcg aaatttcatt cgaaatgaga ctcataatcc aactgtaaaa gatcttattg
     5281 gctttggtct tcaagtagcc aaaggcatga aatatcttgc aagcaaaaag tttgtccaca
     5341 gagacttggc tgcaagaaac tgtatgctgg atgaaaaatt cacagtcaag gttgctgatt
     5401 ttggtcttgc cagaaacatg tatgataaag aatactatag tgtacacaac aaaacaggtg
     5461 caaagctgcc agtgaagtgg atggctttgg aaagtctgca aactcaaaag tttaccacca
     5521 agtcagatgt gtggtccttt ggcgtgctcc tctgggagct gatgacaaga ggagccccac
     5581 cttatcctga cgtaaacacc tttgatataa ctgtttactt gttgcaaggg agaagactcc
     5641 tacaacccga atactgccca gaccccttat atgaagtaat gctaaaatgc tggcacccta
     5701 aagccgaaat gcgcccatcc ttttctgaac tggtgtcccg gatatcagcg atcttctcta
     5761 ctttcattgg ggagcactat gtccatgtga acgctactta tgtgaacgta aaatgtgtcg
     5821 ctccgtatcc ttctctgttg tcatcagaag ataacgctga tgatgaggtg gacacacgac
     5881 cagcctcctt ctgggagaca tcataggatc cgcggccgca aggatctgcg atcgctccgg
     5941 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     6001 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     6061 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     6121 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     6181 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     6241 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     6301 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     6361 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     6421 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat
     6481 ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct
     6541 gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat
     6601 gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca
     6661 cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt
     6721 cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg
     6781 cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt
     6841 caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg
     6901 cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt
     6961 cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat
     7021 gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt
     7081 ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc
     7141 cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga
     7201 cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg
     7261 cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc
     7321 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     7381 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     7441 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     7501 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     7561 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7621 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7681 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7741 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7801 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7861 gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag
     7921 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7981 gcctttgtat cagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8101 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag
     8161 aagctatctg gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt
     8221 gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt
     8281 cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac
     8341 tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg
     8401 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     8461 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     8521 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     8581 gggctttggg ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt
     8641 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     8701 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     8761 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     8821 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     8881 tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc
     8941 gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag
     9001 gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat
     9061 gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt
     9121 gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg
     9181 tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac
     9241 aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg
     9301 ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg
     9361 ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg
     9421 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9481 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9541 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9961 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10021 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10081 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10141 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10201 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
    10261 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10321 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11401 cgacacggaa atgttgaata ctcat
//
