LOCUS       V012347                14382 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012347
VERSION     V012347
KEYWORDS    dCAS9-VP64_GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena
            C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F.
  TITLE     Genome-scale transcriptional activation by an engineered CRISPR-Cas9
            complex.
  JOURNAL   Nature 2015;517:583-8.
   PUBMED   25494202
REFERENCE   2  (bases 1 to 14382)
  AUTHORS   Zhang Lab / Addgene #61422
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
            date: "2015"; volume: "517"; pages: "583-8"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2682..3859
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3872..3874
                     /codon_start=1
                     /product="start codon"
                     /label="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             3878..3898
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             3908..8008
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             8063..8083
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             8108..8257
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label="tetrameric repeat of the minimal activation dom"
                     /note="VP64"
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8279..8332
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label="2A peptide from Thosea asigna virus capsid protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8333..9046
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    9074..9662
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             10187..10367
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10399..10623
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10669..11097
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        11111..11440
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        11488..11535
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             11554..11925
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    12058..12191
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(12276..12306)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12321..12342)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12630..13218)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13392..14249)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(14250..14354)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta
     3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3841 tccatttcag gtgtcgtgac gtacggccac catgagcccc aagaagaaga gaaaggtgga
     3901 ggccagcgac aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc
     3961 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     4021 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     4081 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     4141 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     4201 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     4261 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     4321 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     4381 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     4441 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     4501 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     4561 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     4621 caacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     4681 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     4741 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     4801 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     4861 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     4921 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     4981 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     5041 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     5101 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     5161 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     5221 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     5281 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     5341 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     5401 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     5461 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     5521 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     5581 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     5641 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     5701 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     5761 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     5821 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     5881 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     5941 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     6001 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     6061 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     6121 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     6181 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     6241 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     6301 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     6361 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     6421 cgctatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     6481 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     6541 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     6601 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     6661 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     6721 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     6781 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     6841 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     6901 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     6961 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     7021 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     7081 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     7141 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     7201 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     7261 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     7321 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     7381 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     7441 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     7501 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     7561 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     7621 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     7681 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     7741 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     7801 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     7861 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     7921 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     7981 gatcgacctg tctcagctgg gaggcgacag cgctggagga ggtggaagcg gaggaggagg
     8041 aagcggagga ggaggtagcg gacctaagaa aaagaggaag gtggcggccg ctggatccgg
     8101 acgggctgac gcattggacg attttgatct ggatatgctg ggaagtgacg ccctcgatga
     8161 ttttgacctt gacatgcttg gttcggatgc ccttgatgac tttgacctcg acatgctcgg
     8221 cagtgacgcc cttgatgatt tcgacctgga catgctgatt aacgctagcg gcagtggaga
     8281 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagtgagcaa
     8341 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     8401 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     8461 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     8521 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     8581 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     8641 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     8701 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     8761 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     8821 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     8881 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     8941 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     9001 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat
     9061 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     9121 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     9181 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     9241 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     9301 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     9361 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     9421 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     9481 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     9541 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     9601 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     9661 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     9721 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     9781 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     9841 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     9901 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     9961 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
    10021 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
    10081 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
    10141 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
    10201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
    10381 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
    10441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
    10501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
    10561 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
    10621 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
    10681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    10741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    10801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    10861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    10921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    10981 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
    11041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
    11101 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
    11161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
    11221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
    11281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
    11341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
    11401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
    11461 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
    11521 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
    11581 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
    11641 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
    11701 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
    11761 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
    11821 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
    11881 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
    11941 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    12001 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
    12061 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    12121 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    12181 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
    12241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
    12301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    12361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    12421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    12481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    12541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    12601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    12661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    12721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    12781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    12841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    12901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    12961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    13021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    13081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    13141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    13201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    13261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    13321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    13381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    13441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    13501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    13561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    13621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    13681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    13741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    13801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    13861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    13921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    13981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    14041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    14101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    14161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    14221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    14281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    14341 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
