LOCUS       V029004                 6596 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029004
VERSION     V029004
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        156..535
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        536..739
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      764..773
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             773..796
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             803..2695
                     /label="Rhot1(NM_021536)"
                     /note="Rhot1(NM_021536)"
                     /gene="Rhot1"
     misc_feature    2723..3311
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3343..3819
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   3859..3999
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4074..4529
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4811..4915
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4916..5776
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5947..6535
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      721 ggtctatata agcagagctg gtaccggatc ctctagagtc gacgccacca tggattacaa
      781 ggacgacgat gacaaggcta gcatgcgtgc gggccgagtg cggcccctgc gagcctccga
      841 catgaagaaa gacgtgagga tcctgctggt gggagaacct cgagttggga agacatcact
      901 gattatgtcg ctggtcagtg aagaatttcc ggaagaggtt cctccccgag cagaagaaat
      961 caccattcca gctgacgtca cccctgaaag agttccaaca cacattgtcg attattcaga
     1021 agcagaacag agtgatgaac aacttcatca agaaatatca caggctaatg ttatctgcat
     1081 agtttatgct gttaacaaca aacattctat tgataaggta acaagccgat ggattcctct
     1141 cataaatgaa agaacagaca aagacagcag gctgcctttg atattggttg ggaacaaatc
     1201 tgatctggtg gaatatagta gtatggagac catccttcca attatgaacc agtatacaga
     1261 gatagagacc tgtgtggagt gttcagcaaa aaacctaaag aatatctcag agctgtttta
     1321 ttatgcacag aaagctgttc ttcatcctac agggcctcta tactgcccag aggagaaaga
     1381 gatgaaaccg gcttgtataa aggccctcac tcgtatattt aaaatatctg atcaggataa
     1441 cgacggcact ctgaatgatg ctgagctcaa cttcttccag agaatttgtt tcaacactcc
     1501 tttagctccg caagcgctgg aagatgtcaa gaatgtggtt agaaagcact taagtgacgg
     1561 tgtggctgac agtgggctga cgctcagagg ttttctcttt ttacatacac tttttatcca
     1621 gagggggagg catgagacta cttggactgt gcttcgacga tttggttatg acgatgacct
     1681 ggacctgacg cctgagtatt tattccccct gctaaaaata cctcctgatt gcactactga
     1741 attaaatcat catgcatatt tgtttctcca gagcaccttt gacaaacatg atttggacag
     1801 agactgtgct ttgtcgcctg atgaacttaa agatttattt caagtttttc cttacatacc
     1861 ttggggacca gatgtaaata acacagtttg cacaaatgaa agaggctgga taacctacca
     1921 ggggttcctc tcccagtgga cgctcaccac ctacttagat gtgcagcgtt gcctggagta
     1981 tttgggatat ctaggctatt ccatactgac ggaacaagag tctcaagctt cagccattac
     2041 agtaacaagg gataaaaaga ttgacctgca gaaaaaacag actcagagaa atgtgttcag
     2101 gtgtaatgta attggagtga aaggctgtgg gaaaactgga gttcttcagt ctctccttgg
     2161 aagaaaccta atgaggcaga agaaaattcg tgatgatcat aagtcctatt atgcaattaa
     2221 cactgtctat gtatatggac aagagaaata cttgttgctg cacgatatct cagagtcgga
     2281 atttctaact gaggctgaga ccatttgtga tgttgtatgc ctggtatatg atgtcaccaa
     2341 tcccaaatcc tttgaatact gtgccaggat ttttaagcaa cattttatgg acagcagaat
     2401 tccctgcttg attgtagctg caaagtcaga cctgcatgaa gtcaaacagg aacatagcat
     2461 ctcacctact gatttctgca ggaaacacaa aatgcctcca cctcaagcct tcacttgcaa
     2521 tactgctgat gcccccagta aggatatctt tgttaagttg acaacaatgg ccatgtaccc
     2581 gcacgtgaca caagctgacc tcaagagctc aacgttctgg cttcgcgcaa gctttggtgc
     2641 tacggtcttt gcagtcgtgg gctttgctat gtacagagca ctactgaaac agcgatgaga
     2701 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2761 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2821 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2881 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2941 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3001 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3061 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3121 atcgtccttt ccttggctgc tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt
     3181 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3241 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3301 cgcctccccg catcgatacc gagcgctgct cgagagatct acgggtggca tccctgtgac
     3361 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     3421 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     3481 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     3541 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3601 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     3661 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     3721 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     3781 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttgta ggtaaccacg
     3841 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     3901 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     3961 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4021 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     4081 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4141 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4201 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4261 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     4321 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4381 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
     4441 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4501 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     4561 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     4621 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     4681 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     4741 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4801 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4861 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4921 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4981 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5041 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5101 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5161 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5221 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5281 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5341 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5401 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5461 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5521 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5581 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5641 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5701 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5761 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5821 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     5881 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5941 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6001 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6061 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     6121 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6181 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6241 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6301 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6361 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6421 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6481 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6541 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
