LOCUS       V042619                 6482 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042619
VERSION     V042619
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        236..539
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        540..743
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             862..1818
                     /label="Zfp36(NM_011756)"
                     /note="Zfp36(NM_011756)"
                     /gene="Zfp36"
     CDS             1861..2580
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     misc_feature    2609..3197
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3229..3705
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   3745..3885
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      3960..4415
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4697..4801
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4802..5662
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5833..6421
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagaacgcgt ggagctagtt
      181 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      721 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
      781 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg taccgaaaac
      841 cccggtccgg ctagcgccac catggatctc tctgccatct acgagagcct ccagtcgatg
      901 agccatgacc tgtcatccga ccacggagga accgaatccc tcggaggact ttggaacata
      961 aactcggact ccatcccgtc tggggtcacc tctcgcctga ctggccgctc cactagcctg
     1021 gtggagggcc gaagctgtgg ctgggtaccc ccaccccctg gttttgcacc tttggctccc
     1081 cgcccaggcc ctgagctgtc accctcacct acttcgccta ctgcaactcc caccacctcc
     1141 tctcgataca agaccgagct ctgtcggacc tactcagaaa gcgggcgttg tcgctacggg
     1201 gccaagtgcc agtttgctca cggcctgggt gaacttcgcc aagccaatcg ccaccccaag
     1261 tacaaaacgg aactctgcca caagttctac ctccagggcc gctgccccta tggctctcga
     1321 tgccacttca tccacaaccc caccgaggac ctagctctcc ctggccagcc ccatgtgctg
     1381 cgacaaagca tcagcttctc cggcttgccc tcaggccgca gaagctcgcc gccacctcca
     1441 ggcttttctg gcccttccct gtcctcttgt tccttttcgc cttccagctc cccaccgccc
     1501 cctggggatc ttccactttc cccttctgcc ttctctgctg cccctgggac ccctgtgact
     1561 cgaagagacc ctaaccaggc ctgttgcccc tcctgccgaa ggtctactac ccccagcacc
     1621 atctgggggc ccttgggtgg cctggctcgg agcccatctg cccactctct gggatccgat
     1681 cctgatgact acgccagcag cggcagcagc ctgggggggt cagactcacc tgtctttgag
     1741 gcaggggtgt ttgggcctcc ccagacccct gcacccccaa ggcgtctccc catcttcaat
     1801 cgtatctctg tctctgaggg tggaggcgga tcaggaggcg gtggatctga attcgccacc
     1861 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1921 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     1981 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2041 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2101 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2161 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2221 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2281 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     2341 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     2401 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     2461 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     2521 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     2581 ggatccaagc tttaaaccgg ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2641 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2701 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2761 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2821 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2881 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2941 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3001 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3061 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3121 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3181 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     3241 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     3301 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3361 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3421 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3481 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3541 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3601 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3661 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     3721 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     3781 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3841 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     3901 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     3961 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4021 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4081 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4141 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4201 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4261 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     4321 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4381 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4441 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4501 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4561 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4621 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4801 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4861 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4921 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4981 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5041 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5101 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5161 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5221 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5281 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5341 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5401 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5461 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5521 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5581 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5641 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5701 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5941 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6481 gt
//
