LOCUS pAAV-Yeats4(mouse) 5335 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V044970 VERSION V044970 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 221..524 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 525..727 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 876..1254 /note="fusion between human cytomegalovirus intron A and human β-globin intron 2" /label="chimeric intron" CDS 1338..2018 /label="Yeats4(NM_026570)" /note="Yeats4(NM_026570)" /gene="Yeats4" polyA_signal 2082..2558 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 2598..2738 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 2813..3268 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3550..3654 /gene="bla" /label="AmpR promoter" CDS 3655..4515 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 4686..5274 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cgccaccatg ttcaagagaa tggcggaatt tggacctgac tccggcggga 1381 gagtgaaggg ggttaccatc gttaagccaa tcgtttatgg caatgttgcc aggtactttg 1441 gaaagaagag agaagaagac gggcacactc accagtggac tgtgtacgtg aagccctaca 1501 gaaacgagga tatgtcagca tatgtgaaga agatccagtt taaattacac gaaagctacg 1561 gcaatcctct aagagtcgtc accaagcctc catatgaaat cacagaaaca ggatggggtg 1621 aatttgaaat catcatcaag atatttttca ttgaccccaa cgagagacct gtgactctgt 1681 accacttact gaagctcttc cagtctgaca ccaatgccat gctggggaag aaaacagtgg 1741 tttcagagtt ctatgacgaa atgatatttc aagacccaac ggcaatgatg cagcagctgc 1801 ttacgacgtc tcgccagctg acattgggag cctataagca cgaaacagag tttgcagaac 1861 ttgaagtgaa aaccagagaa aaattagaag ctgccaagaa aaaaacaagc tttgagattg 1921 cggagcttaa ggagagatta aaagcaagtc gtgaaactat aaactgttta aaaaatgaaa 1981 tcaggaagct tgaagaagac gatcagacga aagacatttg aggatcctct agagtcgacc 2041 tgcagaagct tgcctcgagc agcgctgctc gagagatcta cgggtggcat ccctgtgacc 2101 cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc 2161 taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt 2221 ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg 2281 tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct 2341 cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca 2401 tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca 2461 ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg 2521 gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt 2581 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc 2641 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 2701 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct 2761 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc 2821 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 2881 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 2941 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3001 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc 3061 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3121 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt 3181 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3241 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat 3301 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 3361 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 3421 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 3481 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 3541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 3601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 3661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 3721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 3841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 3901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 3961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 4021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 4081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 4141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 4201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 4261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 4321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 4381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 4441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 4501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 4561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 4681 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 4741 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 4801 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 4861 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 4921 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 4981 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5041 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5101 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5161 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5221 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5281 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt //