LOCUS       pAB-6xHis-MBP.       10833 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of proteins 
            with a thrombin-cleavable N-terminal 6xHis-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-6xHis-MBP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4138..4140
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             4147..4164
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             4192..5283
                     /label=MBP
                     /note="maltose binding protein from E. coli"
     CDS             5302..5319
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     misc_recomb     5848..7303
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(8262..8292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8307..8328)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8616..9204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9378..10235)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10236..10340)
                     /label=AmpR promoter
     primer_bind     10814..10830
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atccaaaatg
     4141 agcggtcacc atcaccacca tcacggtgat caactagtag aagaaatggc cgaagaaggt
     4201 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
     4261 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
     4321 aaattcccac aggttgcggc aacaggcgat ggccctgaca ttatcttctg ggcacacgac
     4381 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
     4441 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
     4501 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
     4561 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
     4621 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
     4681 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
     4741 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
     4801 gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
     4861 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
     4921 gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
     4981 attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
     5041 actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
     5101 tcttacgagg aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag
     5161 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
     5221 gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
     5281 actaattcga gctctgcagg cctagttcct cgtggatctc tagatggcgg aattcatggc
     5341 tcccgggcag agcggccgct gcagatctga tcctttcctg ggacccggca agaaccaaaa
     5401 actcactctc ttcaaggaaa tccgtaatgt taaacccgac acgatgaagc ttgtcgttgg
     5461 atggaaagga aaagagttct acagggaaac ttggacccgc ttcatggaag acagcttccc
     5521 cattgttaac gaccaagaag tgatggatgt tttccttgtt gtcaacatgc gtcccactag
     5581 acccaaccgt tgttacaaat tcctggccca acacgctctg cgttgcgacc ccgactatgt
     5641 acctcatgac gtgattagga tcgtcgagcc ttcatgggtg ggcagcaaca acgagtaccg
     5701 catcagcctg gctaagaagg gcggcggctg cccaataatg aaccttcact ctgagtacac
     5761 caactcgttc gaacagttca tcgatcgtgt catctgggag aacttctaca agcccatcgt
     5821 ttacatcggt accgactctg ctgaagagga ggaaattctc cttgaagttt ccctggtgtt
     5881 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     5941 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     6001 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     6061 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     6121 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     6181 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     6241 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     6301 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     6361 aaacacgtta aataaagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     6421 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     6481 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     6541 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
     6601 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
     6661 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
     6721 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
     6781 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
     6841 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
     6901 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
     6961 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
     7021 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
     7081 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
     7141 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
     7201 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
     7261 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctccgcacgc cgataacaag
     7321 ccttttcatt tttactacag cattgtagtg gcgagacact tcgctgtcgt cgacgtacat
     7381 gtatgctttg ttgtcaaaaa cgtcgttggc aagctttaaa atatttaaaa gaacatctct
     7441 gttcagcacc actgtgttgt cgtaaatgtt gtttttgata atttgcgctt ccgcagtatc
     7501 gacacgttca aaaaattgat gcgcatcaat tttgttgttc ctattattga ataaataaga
     7561 ttgtacagat tcatatctac gattcgtcat ggccaccaca aatgctacgc tgcaaacgct
     7621 ggtacaattt tacgaaaact gcaaaaacgt caaaactcgg tataaaataa tcaacgggcg
     7681 ctttggcaaa atatctattt tatcgcacaa gcccactagc aaattgtatt tgcagaaaac
     7741 aatttcggcg cacaatttta acgctgacga aataaaagtt caccagttaa tgagcgacca
     7801 cccaaatttt ataaaaatct attttaatca cggttccatc aacaaccaag tgatcgtgat
     7861 ggactacatt gactgtcccg atttatttga aacactacaa attaaaggcg agctttcgta
     7921 ccaacttgtt agcaatatta ttagacagct gtgtgaagcg ctcaacgatt tgcacaagca
     7981 caatttcata cacaacgaca taaaactcga aaatgtctta tatttcgaag cacttgatcg
     8041 cgtgtatgtt tgcgattacg gattgtgcaa acacgaaaac tcacttagcg tgcacgacgg
     8101 cacgttggag tattttagtc cggaaaaaat tcgacacaca actatgcacg tttcgtttga
     8161 ctggtacgcg gcgtgttaac atacaagttg ctaaccggcg gttcgtaatc atggtcatag
     8221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10381 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10441 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10501 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10561 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10621 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    10681 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    10741 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    10801 cagtcacgac gttgtaaaac gacggccagt gcc
//
