LOCUS       pAB-bee.        9932 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid for high-level expression of secreted 
            proteins with a C-terminal 8xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pAB-bee.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4143..4205
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     CDS             4418..4441
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_recomb     4947..6402
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        complement(7361..7391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7406..7427)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7715..8303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8477..9334)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9335..9439)
                     /label=AmpR promoter
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcagatcta
     4141 aaatgaaatt cttagtcaac gttgcccttg tttttatggt cgtatacatt tcttacatct
     4201 atgcggccgc tccggaattc tagaaggtac ccgggatccc actgaagatg atgacatgaa
     4261 gatgaagatg atgatggtga tgacatcgat gccgatgatg acgacgacga tgataatgat
     4321 aattctgatg aggataatga agacagtgat gatgacgatg atgacgatga atagctccag
     4381 ccctgggtga ttctgatgag tgacagaatt cgtcgagcac caccaccacc accaccacca
     4441 ctaattgatt aatacctagg tcgacgcgat cctttcctgg gacccggcaa gaaccaaaaa
     4501 ctcactctct tcaaggaaat ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga
     4561 tggaaaggaa aagagttcta cagggaaact tggacccgct tcatggaaga cagcttcccc
     4621 attgttaacg accaagaagt gatggatgtt ttccttgttg tcaacatgcg tcccactaga
     4681 cccaaccgtt gttacaaatt cctggcccaa cacgctctgc gttgcgaccc cgactatgta
     4741 cctcatgacg tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc
     4801 atcagcctgg ctaagaaggg cggcggctgc ccaataatga accttcactc tgagtacacc
     4861 aactcgttcg aacagttcat cgatcgtgtc atctgggaga acttctacaa gcccatcgtt
     4921 tacatcggta ccgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc
     4981 aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg
     5041 atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
     5101 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
     5161 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
     5221 gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct
     5281 ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat
     5341 ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa
     5401 atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta
     5461 aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga
     5521 ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata
     5581 gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag
     5641 ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc
     5701 gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac
     5761 ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc
     5821 gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt
     5881 ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg
     5941 ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct
     6001 tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt
     6061 ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta
     6121 ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt
     6181 tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg
     6241 cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa
     6301 aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc
     6361 tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tccgcacgcc gataacaagc
     6421 cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gacgtacatg
     6481 tatgctttgt tgtcaaaaac gtcgttggca agctttaaaa tatttaaaag aacatctctg
     6541 ttcagcacca ctgtgttgtc gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg
     6601 acacgttcaa aaaattgatg cgcatcaatt ttgttgttcc tattattgaa taaataagat
     6661 tgtacagatt catatctacg attcgtcatg gccaccacaa atgctacgct gcaaacgctg
     6721 gtacaatttt acgaaaactg caaaaacgtc aaaactcggt ataaaataat caacgggcgc
     6781 tttggcaaaa tatctatttt atcgcacaag cccactagca aattgtattt gcagaaaaca
     6841 atttcggcgc acaattttaa cgctgacgaa ataaaagttc accagttaat gagcgaccac
     6901 ccaaatttta taaaaatcta ttttaatcac ggttccatca acaaccaagt gatcgtgatg
     6961 gactacattg actgtcccga tttatttgaa acactacaaa ttaaaggcga gctttcgtac
     7021 caacttgtta gcaatattat tagacagctg tgtgaagcgc tcaacgattt gcacaagcac
     7081 aatttcatac acaacgacat aaaactcgaa aatgtcttat atttcgaagc acttgatcgc
     7141 gtgtatgttt gcgattacgg attgtgcaaa cacgaaaact cacttagcgt gcacgacggc
     7201 acgttggagt attttagtcc ggaaaaaatt cgacacacaa ctatgcacgt ttcgtttgac
     7261 tggtacgcgg cgtgttaaca tacaagttgc taaccggcgg ttcgtaatca tggtcatagc
     7321 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7381 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7441 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8461 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8521 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8581 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8641 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8701 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8761 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8821 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8881 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8941 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9001 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9061 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9121 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9181 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9241 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9301 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9361 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9421 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9481 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     9541 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9601 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9661 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9721 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     9781 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9841 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9901 agtcacgacg ttgtaaaacg acggccagtg cc
//
