LOCUS       40924_4409        5255 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Saccharomyces cerevisiae vector pAM656, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Self-excising integrative yeast plasmid vectors containing an 
            intronated recombinase gene
  JOURNAL   FEMS Yeast Res. (2014) In press
  PUBMED    25124534
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Agaphonov M, Alexandrov A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian
            Academy of Science, Moscow 119071, Russian Federation
REFERENCE   3  (bases 1 to 5255)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAY-2014) A.N. Bach Institute of Biochemistry, Russian Academy 
            of Science, Moscow 119071, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..38)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    51..84
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(109..550)
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     intron          684..734
     CDS             738..1721
                     /label=Cre
                     /note="site-specific recombinase"
     rep_origin      complement(1797..2385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2559..3416)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3417..3521)
                     /label=AmpR promoter
     promoter        3627..4029
                     /label=LEU2 promoter
     CDS             4030..5121
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     misc_recomb     complement(5195..5228)
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(5195..5228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 ggaattcgat atcaagctta tcgataccgt cgacctcgac aacccttaat ataacttcgt
       61 ataatgtatg ctatacgaag ttattaggtc tagaatccgg ggttttttct ccttgacgtt
      121 aaagtataga ggtatattaa caattttttg ttgatacttt tattacattt gaataagaag
      181 taatacaaac cgaaaatgtt gaaagtatta gttaaagtgg ttatgcagtt tttgcattta
      241 tatatctgtt aatagatcaa aaatcatcgc ttcgctgatt aattacccca gaaataaggc
      301 taaaaaacta atcgcattat catcctatgg ttgttaattt gattcgttca tttgaaggtt
      361 tgtggggcca ggttactgcc aatttttcct cttcataacc ataaaagcta gtattgtaga
      421 atctttattg ttcggagcag tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg
      481 tgaagacgag gacgcacgga ggagagtctt ccttcggagg gctgtcaccc gctcggcggc
      541 ttctaatccg tactagaggc tgccgctaag tgtacccaac cgtccccaca cggtccatct
      601 ataaatactg ctgccagtgc acggtggtga catcaatcca aagttactac aagacaaata
      661 gacaagtcaa gttagataaa atggtaagtt gccaatgcca gaggcagcat gtgcaagtgt
      721 tctaaccttt ctagtcaatc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca
      781 tgttcaggga tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc
      841 ggtcgtgggc ggcatggtgc aagttgaata accgggaatg gtttcccgca gaacctgaag
      901 atgttcgcgg ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
      961 aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca
     1021 gcaatgctgt ttcactggtt atgcggcgga tcagaaaaga aaacgttgat gccggtgaac
     1081 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
     1141 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca
     1201 ccctgttacg tatagccgaa attgccagga tcagggttaa agacatctca cgtactgacg
     1261 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
     1321 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
     1381 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
     1441 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
     1501 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
     1561 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
     1621 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
     1681 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga ttagccatta acgcgccatg
     1741 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1801 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1861 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1921 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1981 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2041 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2101 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2161 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2221 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2281 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2401 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2461 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2521 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2581 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2641 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2701 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2761 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2821 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2881 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2941 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3001 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3061 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ttcaaccaag
     3121 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3181 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3241 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3301 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3361 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3421 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3481 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3541 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3601 acgaggccct ttcgtcttca agaattaact gtgggaatac tcaggtatcg taagatgcaa
     3661 gagttcgaat ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg
     3721 cgctatcgca cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc
     3781 tgacgcatat acctttttca actgaaaaat tgggagaaaa aggaaaggtg agaggccgga
     3841 accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat cacaatactt
     3901 gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt agcaatcgtc
     3961 ttattctaac ttttcttacc ttttacattt cagcaatata tatatatatt tcaaggatat
     4021 accattctaa tgtctgcccc taagaagatc gtcgttttgc caggtgacca cgttggtcaa
     4081 gaaatcacag ccgaagccat taaggttctt aaagctattt ctgatgttcg ttccaatgtc
     4141 aagttcgatt tcgaaaatca tttaattggt ggtgctgcta tcgatgctac aggtgtccca
     4201 cttccagatg aggcgctgga agcctccaag aaggttgatg ccgttttgtt aggtgctgtg
     4261 ggtggtccta aatggggtac aggtagtgtt agacctgaac aaggtttact aaaaatccgt
     4321 aaagaacttc aattgtacgc caacttaaga ccatgtaact ttgcatccga ctctctttta
     4381 gacttatctc caatcaagcc acaatttgct aaaggtactg acttcgttgt tgtcagagaa
     4441 ttagtgggag gtatttactt tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg
     4501 gatagtgaac aatacaccgt tccagaagtg caaagaatca caagaatggc cgctttcatg
     4561 gccctacaac atgagccacc attgcctatt tggtccttgg ataaagctaa tgttttggcc
     4621 tcttcaagat tatggagaaa aactgtggag gaaaccatca agaacgaatt tcctacattg
     4681 aaggttcaac atcaattgat tgattctgcc gccatgatcc tagttaagaa cccaacccac
     4741 ctaaatggta ttatcatcac cagcaatatg tttggtgaca tcatctccga tgaagcctcc
     4801 gttatcccag gttccttggg tttgttgcca tctgcgtcct tggcctcttt gccagacaag
     4861 aacaccgcat ttggtttgta cgaaccatgc cacggttctg ctccagattt gccaaagaat
     4921 aaggttgacc ctatcgccac tatcttgtct gctgcaatga tgttgaaatt gtcattgaac
     4981 ttgcctgaag aaggtaaggc cattgaagat gcagttaaaa aggttttgga tgcaggtatc
     5041 agaactggtg atttaggtgg ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa
     5101 gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacaccttg
     5161 tacgcagcgt cgaagccggt cgagaaccct taatataact tcgtataatg tatgctatac
     5221 gaagttatta ggtgatctcc accgcggtgg cggcc
//
