LOCUS       pAMP10.        4122 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of a cloned cDNA. For an 
            MCS with a NotI site, use pAMP1.
ACCESSION   .
VERSION     .
KEYWORDS    pAMP10.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     140..156
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        164..182
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    191..299
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(313..331)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(350..366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(374..390)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(398..428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(443..464)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(480..1559)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1560..1637)
                     /label=lacI promoter
     rep_origin      complement(1915..2503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2677..3534)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3535..3639)
                     /label=AmpR promoter
     rep_origin      complement(3666..4121)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
       61 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      121 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
      181 gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccggcc
      241 gcctactact actaatgatg atgatgtcga cccgggaatt ccggaccggt acctgcaggc
      301 gtaccagctt tccctatagt gagtcgtatt agagcttggc gtaatcatgg tcatagctgt
      361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      541 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
      601 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
      661 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttgacggcgg gatataacat
      721 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
      781 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
      841 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
      901 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
      961 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     1021 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     1081 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     1141 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     1201 ctgacccgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     1261 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     1321 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     1381 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     1441 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     1501 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     1561 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     1621 cgccattcga tggtgtcaac gtaaatgccg cttcgccttc gcgcgcgaat tgcaagctct
     1681 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1741 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2101 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2341 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3661 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3721 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3781 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3841 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3901 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3961 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4021 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4081 caccacaccc gccgcgctta atgcgccgct acagggcgcg tc
//
