LOCUS       pAcAB4.       10230 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expressing up to four proteins 
            simultaneously, for use with the BD BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcAB4.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..578
                     /gene="PTP"
                     /label=PTP
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2661..3290
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3169..3996
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     polyA_signal    complement(4000..4133)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4462..4571)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        complement(4722..4813)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        4838..4947
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        5000..5091
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_recomb     5101..6458
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        8094..8198
                     /label=AmpR promoter
     CDS             8199..9056
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9230..9818
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10106..10127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10142..10172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10180..10196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10204..10220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattctactc gtaaagcgag ttgaaggatc atatttagtt gcgtttatga gataagattg
       61 aaagcacgtg taaaatgttt cccgcgcgtt ggcacaacta tttacaatgc ggccaagtta
      121 taaaagattc taatctgata tgttttaaaa cacctttgcg gcccgagttg tttgcgtacg
      181 tgactagcga agaagatgtg tggaccgcag aacagatagt aaaacaaaac cctagtattg
      241 gagcaataat cgatttaacc aacacgtcta aatattatga tggtgtgcat tttttgcggg
      301 cgggcctgtt atacaaaaaa attcaagtac ctggccagac tttgccgcct gaaagcatag
      361 ttcaagaatt tattgacacg gtaaaagaat ttacagaaaa gtgtcccggc atgttggtgg
      421 gcgtgcactg cacacacggt attaatcgca ccggttacat ggtgtgcaga tatttaatgc
      481 acaccctggg tattgcgccg caggaagcca tagatagatt cgaaaaagcc agaggtcaca
      541 aaattgaaag acaaaattac gttcaagatt tattaattta attaatatta tttgcattct
      601 ttaacaaata ctttatccta ttttcaaatt gttgcgcttc ttccagcgaa ccaaaactat
      661 gcttcgcttg ctccgtttag cttgtagccg atcagtggcg ttgttccaat cgacggtagg
      721 attaggccgg atattctcca ccacaatgtt ggcaacgttg atgttacgtt tatgcttttg
      781 gttttccacg tacgtctttt ggccggtaat agccgtaaac gtagtgccgt cgcgcgtcac
      841 gcacaacacc ggatgtttgc gcttgtccgc ggggtattga accgcgcgat ccgacaaatc
      901 caccactttg gcaactaaat cggtgacctg cgcgtctttt ttctgcatta tttcgtcttt
      961 cttttgcatg gtttcctgga agccggtgta catgcggttt agatcagtca tgacgcgcgt
     1021 gacctgcaaa tctttggcct cgatctgctt gtccttgatg gcaacgatgc gttcaataaa
     1081 ctcttgtttt ttaacaagtt cctcggtttt ttgcgccacc accgcttgca gcgcgtttgt
     1141 gtgctcggtg aatgtcgcaa tcagcttagt caccaactgt ttgctctcct cctcccgttg
     1201 tttgatcgcg ggatcgtact tgccggtgca gagcacttga ggaattactt cttctaaaag
     1261 ccattcttgt aattctatgg cgtaaggcaa tttggacttc ataatcagct gaatcacgcc
     1321 ggatttagta atgagcactg tatgcggctg caaatacagc gggtcgcccc ttttcacgac
     1381 gctgttagag gtagggcccc cattttggat ggtctgctca aataacgatt tgtatttatt
     1441 gtctacatga acacgtatag ctttatcaca aactgtatat tttaaactgt tagcgacgtc
     1501 cttggccacg aaccggacct gttggtcgcg ctctagcacg taccgcaggt tgaacgtatc
     1561 ttctccaaat ttaaattctc caattttaac gcgagccatt ttgatacacg tgtgtcgatt
     1621 ttgcaacaac tattgttttt taacgcaaac taaacttatt gtggtaagca ataattaaat
     1681 atgggggaac atgcgccgct acaacactcg tcgttatgaa cgcagacggc gccggtctcg
     1741 gcgcaagcgg ctaaaacgtg ttgcgcgttc aacgcggcaa acatcgcaaa agccaatagt
     1801 acagttttga tttgcatatt aacggcgatt ttttaaatta tcttatttaa taaatagtta
     1861 tgacgcctac aactccccgc ccgcgttgac tcgctgcacc tcgagcagtt cgttgacgcc
     1921 ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg accagcggcg tgccgcacgc
     1981 gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa ggcacgtcgg cctccaagtg
     2041 gcaatattgg caaattcgaa aatatataca gttgggttgt ttgcgcatat ctatcgtggc
     2101 gttgggcatg tacgtccgaa cgttgatttg catgcaagcc gaaattaaat cattgcgatt
     2161 agtgcgatta aaacgttgta catcctcgct tttaatcatg ccgtcgatta aatcgcgcaa
     2221 tcgagtcaag tgatcaaagt gtggaataat gttttctttg tattcccgag tcaagcgcag
     2281 cgcgtatttt aacaaactag ccatcttgta agttagtttc atttaatgca actttatcca
     2341 ataatatatt atgtatcgca cgtcaagaat taacaatgcg cccgttgtcg catctcaaca
     2401 cgactatgat agagatcaaa taaagcgcga attaaatagc ttgcgacgca acgtgcacga
     2461 tctgtgcacg cgttccggca cgagctttga ttgtaataag tttttacgaa gcgatgacat
     2521 gacccccgta gtgacaacga tcacgcccaa aagaactgcc gactacaaaa ttaccgagta
     2581 tgtcggtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
     2641 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
     2701 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
     2761 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
     2821 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
     2881 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
     2941 atgtcgtcga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
     3001 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
     3061 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
     3121 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
     3181 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc
     3241 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     3301 aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     3361 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     3421 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     3481 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     3541 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     3601 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     3661 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     3721 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc cggcatagta
     3781 cgcagcttct tctagttcaa ttacaccatt ttttagcagc accggattaa cataactttc
     3841 caaaatgttg tacgaaccgt taaacaaaaa cagttcacct cccttttcta tactattgtc
     3901 tgcgagcagt tgtttgttgt taaaaataac agccattgta atgagacgca caaactaata
     3961 tcacaaactg gaaatgtcta tcaatatata gttgctgatg atccagcatg ataagataca
     4021 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4081 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttccgagtt
     4141 tgtcagaaag cagaccaaac agcggttgga ataatagcga gaacagagaa atagcggcaa
     4201 aaataatacc cgtatcactt ttgctgatat ggttgatgtc atgtagccaa atcgggaaaa
     4261 acgggaagta ggctcccatg ataaaaaagt aaaagaaaaa gaataaaccg aacatccaaa
     4321 agtttgtgtt ttttaaatag tacataatgg atttccttac gcgaaatacg ggcagacatg
     4381 gcctgcccgg ttattattat ttttgacacc agaccaactg gtaatggtag cgaccggcgc
     4441 tcagctggaa ttcagatctg tgattgtaaa taaaatgtaa tttacagtat agtattttaa
     4501 ttaatataca aatgatttga taataattct tatttaacta taatatattg tgttgggttg
     4561 aattaaaggt cccggcatcc tcaaatgcat aatttcatag tcccccttgt tgtaagtgat
     4621 gcgtatttct gaatctttgt aaaatagcac acaagactcc aacgcgtttg gcgttttatt
     4681 ttcttgctcg actctagttt attaggcctc tagagatccg tatttatagg tttttttatt
     4741 acaaaactgt tacgaaaaca gtaaaatact tatttatttg cgagatggtt atcattttaa
     4801 ttatctccat gatccaataa cctagaataa agggcccgac ctttaattca acccaacaca
     4861 atatattata gttaaataag aattattatc aaatcatttg tatattaatt aaaatactat
     4921 actgtaaatt acattttatt tacaatcaca gatccccggg actagtaata aaacctagag
     4981 gatcgatccc ccggatctga tcatggagat aattaaaatg ataaccatct cgcaaataaa
     5041 taagtatttt actgttttcg taacagtttt gtaataaaaa aacctataaa tacggatccg
     5101 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     5161 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     5221 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     5281 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     5341 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     5401 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     5461 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     5521 taaataaagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     5581 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     5641 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     5701 gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta
     5761 attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat
     5821 ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg
     5881 gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct
     5941 caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg
     6001 tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca
     6061 attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg
     6121 gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg
     6181 gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca
     6241 cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag
     6301 gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg
     6361 ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt
     6421 aagcaattgt attgtaaaga gattgtctca agctcggatc gatcccgcac gccgataaca
     6481 agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgacgtac
     6541 atgtatgctt tgttgtcaaa aacgtcgttg gcaagcttta aaatatttaa aagaacatct
     6601 ctgttcagca ccactgtgtt gtcgtaaatg ttgtttttga taatttgcgc ttccgcagta
     6661 tcgacacgtt caaaaaattg atgcgcatca attttgttgt tcctattatt gaataaataa
     6721 gattgtacag attcatatct acgattcgtc atggccacca caaatgctac gctgcaaacg
     6781 ctggtacaat tttacgaaaa ctgcaaaaac gtcaaaactc ggtataaaat aatcaacggg
     6841 cgctttggca aaatatctat tttatcgcac aagcccacta gcaaattgta tttgcagaaa
     6901 acaatttcgg cgcacaattt taacgctgac gaaataaaag ttcaccagtt aatgagcgac
     6961 cacccaaatt ttataaaaat ctattttaat cacggttcca tcaacaacca agtgatcgtg
     7021 atggactaca ttgactgtcc cgatttattt gaaacactac aaattaaagg cgagctttcg
     7081 taccaacttg ttagcaatat tattagacag ctgtgtgaag cgctcaacga tttgcacaag
     7141 cacaatttca tacacaacga cataaaactc gaaaatgtct tatatttcga agcacttgat
     7201 cgcgtgtatg tttgcgatta cggattgtgc aaacacgaaa actcacttag cgtgcacgac
     7261 ggcacgttgg agtattttag tccggaaaaa attcgacaca caactatgca cgtttcgttt
     7321 gactggtacg cggcgtgtta acatacaagt tgctaaccgg cggccgacac ccatttgaaa
     7381 aaagcgaaga cgaaatgttg gacttgaata gcatgaagcg tcgtcagcaa tacaatgaca
     7441 ttggcgtttt aaaacacgtt cgtaacgtta acgctcgtga ctttgtgtac tgcctaacaa
     7501 gatacaacat agattgtaga ctcacaaatt acaaacaaat tataaaacat gagtttttgt
     7561 cgtaaaaatg ccacttgttt tacgagtaga attcccagct tggcactggc cgtcgtttta
     7621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7741 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     7801 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7861 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     7921 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     7981 tcatcaccga aacgcgcgga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8041 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9361 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9601 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10021 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10081 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10141 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10201 acacaggaaa cagctatgac catgattacg
//
