LOCUS       pAcG3X.        8534 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expressing N-terminally GST-tagged 
            proteins with a Factor Xa cleavage site, for use with the BD 
            BaculoGold(TM) system.
ACCESSION   .
VERSION     .
KEYWORDS    pAcG3X.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             761..1390
                     /gene="LEF-2"
                     /label=LEF-2
                     /note="Primase-associated factor LEF-2 from Autographa 
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     1269..2096
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     promoter        2100..2191
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2206..2859
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             2869..2880
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    2882..2897
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     2927..4284
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     primer_bind     complement(5428..5444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5657..6112
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6394..6498
                     /label=AmpR promoter
     CDS             6499..7356
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7530..8118
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8406..8427
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8442..8472
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8480..8496
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8504..8520
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatacta
     2221 ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga atatcttgaa
     2281 gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag
     2341 tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga tgttaaatta
     2401 acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt gggtggttgt
     2461 ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat tagatacggt
     2521 gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt tcttagcaag
     2581 ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata tttaaatggt
     2641 gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt tttatacatg
     2701 gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct
     2761 atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc tttgcagggc
     2821 tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctgat cgaaggtcgt
     2881 gggatccccg ggaattcatc gtgactgact gacgatctga gatccggtta ttagtacatt
     2941 tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat
     3001 aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca
     3061 gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga
     3121 ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg
     3181 ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt
     3241 tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt
     3301 ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa taaagcttgg
     3361 acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc
     3421 tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt
     3481 gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttggaat tatttctgat
     3541 tgcgggcgtt tttgggcggg tttcaatcta actgtgcccg attttaattc agacaacacg
     3601 ttagaaagcg atggtgcagg cggtggtaac atttcagacg gcaaatctac taatggcggc
     3661 ggtggtggag ctgatgataa atctaccatc ggtggaggcg caggcggggc tggcggcgga
     3721 ggcggaggcg gaggtggtgg cggtgatgca gacggcggtt taggctcaaa tgtctcttta
     3781 ggcaacacag tcggcacctc aactattgta ctggtttcgg gcgccgtttt tggtttgacc
     3841 ggtctgagac gagtgcgatt tttttcgttt ctaatagctt ccaacaattg ttgtctgtcg
     3901 tctaaaggtg cagcgggttg aggttccgtc ggcattggtg gagcgggcgg caattcagac
     3961 atcgatggtg gtggtggtgg tggaggcgct ggaatgttag gcacgggaga aggtggtggc
     4021 ggcggtgccg ccggtataat ttgttctggt ttagtttgtt cgcgcacgat tgtgggcacc
     4081 ggcgcaggcg ccgctggctg cacaacggaa ggtcgtctgc ttcgaggcag cgcttggggt
     4141 ggtggcaatt caatattata attggaatac aaatcgtaaa aatctgctat aagcattgta
     4201 atttcgctat cgtttaccgt gccgatattt aacaaccgct caatgtaagc aattgtattg
     4261 taaagagatt gtctcaagct cggatcgatc ccgcacgccg ataacaagcc ttttcatttt
     4321 tactacagca ttgtagtggc gagacacttc gctgtcgtcg acgtacatgt atgctttgtt
     4381 gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga acatctctgt tcagcaccac
     4441 tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc gcagtatcga cacgttcaaa
     4501 aaattgatgc gcatcaattt tgttgttcct attattgaat aaataagatt gtacagattc
     4561 atatctacga ttcgtcatgg ccaccacaaa tgctacgctg caaacgctgg tacaatttta
     4621 cgaaaactgc aaaaacgtca aaactcggta taaaataatc aacgggcgct ttggcaaaat
     4681 atctatttta tcgcacaagc ccactagcaa attgtatttg cagaaaacaa tttcggcgca
     4741 caattttaac gctgacgaaa taaaagttca ccagttaatg agcgaccacc caaattttat
     4801 aaaaatctat tttaatcacg gttccatcaa caaccaagtg atcgtgatgg actacattga
     4861 ctgtcccgat ttatttgaaa cactacaaat taaaggcgag ctttcgtacc aacttgttag
     4921 caatattatt agacagctgt gtgaagcgct caacgatttg cacaagcaca atttcataca
     4981 caacgacata aaactcgaaa atgtcttata tttcgaagca cttgatcgcg tgtatgtttg
     5041 cgattacgga ttgtgcaaac acgaaaactc acttagcgtg cacgacggca cgttggagta
     5101 ttttagtccg gaaaaaattc gacacacaac tatgcacgtt tcgtttgact ggtacgcggc
     5161 gtgttaacat acaagttgct aaccggcggc cgacacccat ttgaaaaaag cgaagacgaa
     5221 atgttggact tgaatagcat gaagcgtcgt cagcaataca atgacattgg cgttttaaaa
     5281 cacgttcgta acgttaacgc tcgtgacttt gtgtactgcc taacaagata caacatagat
     5341 tgtagactca caaattacaa acaaattata aaacatgagt ttttgtcgta aaaatgccac
     5401 ttgttttacg agtagaatta gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     5461 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     5521 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     5581 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     5641 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5701 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5761 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5821 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     5881 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5941 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     6001 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6061 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     6121 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6181 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6241 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6301 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6361 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6421 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6481 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6541 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6601 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6661 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6721 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6781 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6841 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6901 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6961 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7021 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7081 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7141 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7201 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7261 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7321 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7381 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7441 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7501 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7561 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7621 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     7681 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7741 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7801 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7861 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7921 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7981 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8041 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8101 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8161 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8221 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8281 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8341 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     8401 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8461 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8521 catgattacg aatt
//
