LOCUS Exported 6984 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Expression vector pBCGE7, complete sequence. ACCESSION EF175995 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6984) AUTHORS Govan VA, Williamson AL. TITLE Rabbits immunised with recombinant BCG expressing the cottontail rabbit papillomavirus (CRPV) L2E7E2 genes induces regression of established papillomas JOURNAL Virus Res. 127 (1), 43-48 (2007) PUBMED 17451831 REFERENCE 2 (bases 1 to 6984) AUTHORS Govan VA, Williamson A-L. TITLE Direct Submission JOURNAL Submitted (10-DEC-2006) Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa REFERENCE 3 (bases 1 to 6984) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6984 /organism="Expression vector pBCGE7" /mol_type="other DNA" /label=derived from Cottontail rabbit papillomavirus E7 /note="derived from Cottontail rabbit papillomavirus E7" /db_xref="taxon:419255" CDS 1877..2692 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 3024..3612 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 6556..6879 /codon_start=1 /gene="E7" /product="E7" /label=E7 /protein_id="ABM68359.1" /translation="MIGRTPKLSELVLGETAEALSLHCDEALENLSDDDEEDHQDRQVF IERPYAVSVPCKRCRQTISFVCVCAPEAIRTLNRLLSASLSLVCPECCNLLLLLLIDVD VVN" gene 6556..6879 /gene="E7" /label=E7 terminator 6902..6948 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 gctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt 61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg 121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg 181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc 241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg 301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg 361 atccggagga atcacttcgc aatggccaag acaattgcgg atccagatgt gaacgagctg 421 ctgcacttag cgccgaatgt gtggccgcgc aatactactc gcgatgaagt cggtgtggtc 481 tgcatcgcag gaattccact gacgcagctc gcccaggagt acgggacccc gctgttcgtc 541 atcgacgagg acgactttcg ctcgcgctgc cgagaaaccg ccgcggcctt tggaagtggg 601 gcgaacgtgc actatgccgc caaggcgttc ctgtgcagcg aagtagcccg gtggatcagc 661 gaagaagggc tctgtctgga cgtttgcacc ggtggggagt tggcggtcgc gctgcacgct 721 agctttccgc ccgagcgaat taccttgcac ggcaacaaca aatcggtctc agagttgacc 781 gctgcggtca aagccggagt cggccatatt gtcgtcgatt cgatgaccga gatcgagcgc 841 ctcgacgcca tcgcgggcga ggccggaatc gtccaggatg tcctggtgcg tctcaccgtc 901 ggtgtcgagg cgcacaccca cgagttcatc tccaccgcgc acgaggacca gaaattcggg 961 ttatcggtgg ccagcggcgc ggccatggca gcggtgcggc gcgttttcgc cactgatcac 1021 ctgcgcctgg ttgggctaca cagccacatc ggttcgcaga tcttcgacgt ggacggcttc 1081 gaactcgccg cgcaccgtgt catcggcctg ctacgcgacg tcgtcggcga gttcggtccc 1141 gaaaagacgg cacagatcgc gaccgtcgat ctcggtggcg gcttgggcat ctcgtatttg 1201 ccgtccgacg acccaccgcc gatagccgag ctcgcggcca agctgggtac catcgtgagc 1261 gacgagtcaa cggccgtggg gctgccgacg cccaagctcg ttgtggagcc cggacgcgcc 1321 atcgccggac cgggcaccat cacgttgtat gaggtcggca ccgttaagga cgtcgatgtc 1381 agcgccacag cgcatcgacg ttacgtcagt gtcgacggcg gcatgagcga caacatccgc 1441 accggcctct acggcgcgca gtatgacgtc cggctggtgt ctcgagtcag cgacgccccg 1501 ccggtaccgg cccgtctggt cggaaagcac tgcgaaagtg gcgatatcat cgtgcgggac 1561 acctgggtgc cgacgatatt cggcccggcg atctggttgc ggttgccgcc accggcctta 1621 ctgctattcg ctgtcgagtc gttacaacat ggtcggccgt cccgctgtgg tagcggtgca 1681 cgcggcaacg ctcgcctggt cctgcgtcgg gagacggtcg acgatttgct gagtttggaa 1741 gtgaggtgaa ctagtctcta gccaacaaag cgacgttgtg tctcaaaatc tctgatgtta 1801 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag 1861 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga ggccgcgatt 1921 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 1981 atcaggtgcg acaatctatc gcttgtatgg gaagccccat gcgccagagt tgtttctgaa 2041 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 2101 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 2161 gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga 2221 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 2281 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 2341 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 2401 tgaacaagtc tggaaagaaa tgcataatct tttgccattc tcaccggatt cagtcgtcac 2461 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 2521 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 2581 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 2641 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 2701 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt 2761 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca 2821 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct 2881 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat 2941 gagtcagcaa caccttcttc acgaggcaga cctcactagt tccactgagc gtcagacccc 3001 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 3061 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 3121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 3181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 3241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 3301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 3361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga 3421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 3481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 3541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 3601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 3661 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 3721 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac 3781 cagctccgta agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc 3841 taacgggtct aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc 3901 ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc 3961 gctggtgttt catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg 4021 cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt 4081 ggtttattat caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac 4141 tggtccctgg caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc 4201 ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac 4261 gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc 4321 aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc 4381 cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc 4441 gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac 4501 cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac 4561 atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac 4621 aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg 4681 gcctatcttc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc 4741 cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt 4801 cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc 4861 acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg 4921 cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg 4981 cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg 5041 gctacagcga cggctacaac tggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg 5101 ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg 5161 aacgcagcga gtggttcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg 5221 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg 5281 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa 5341 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg 5401 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg 5461 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgt ccgttttgaa 5521 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga 5581 atatcgtgga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt 5641 gtagtgcttg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa atctagagcg 5701 attgagggcg ttgacggcgc tggcaagcgg acgttggtgg aaaagctgtc cggggccttt 5761 cgagcagccg ggagatcggt ggccacactg gcgttcccgc gctacggaca gtcggtggcc 5821 gccgacatcg cagcggaggc gctgcacggc gagcacggtg acctcgcatc gtcggtgtat 5881 gcgatggcga cgctgttcgc gctcgaccgc gctggcgcgg tccacacgat ccaggggctg 5941 tgtcgcggct acgacgtggt gatcctggat cgctacgtcg cctccaacgc ggcctacagc 6001 gcggcgcgcc tacatgaaaa cgcggccggg aaggcagcgg cctgggttca gcggatcgaa 6061 tttgcaagac tcgggttgcc caagcccgac tggcaggtgc tccttgcggt ctctgccgag 6121 ctcgccgggg aacgatcccg cggccgtgcc cagcgtgacc ccggtcgggc gcgcgacaat 6181 tacgaacgcg acgctgaact tcagcagcgc accggtgcgg tctacgccga gttggcggcc 6241 caagggtggg gcggccggtg gctggttgtc ggcgccgatg ttgatccggg ccgactagcg 6301 gcgactttgg cgcctccaga cgtgccaagt tgagggtatt tgccctaatc aagcaagaaa 6361 cgcccgtgtg gtgcgccagt tttgtcccga tgtggtgaca ccatggacca tatgaagcgt 6421 ggactgacgg tcgcggtagc cggagccgcc attctggtcg caggtctttc cggatgttca 6481 agcaacaagt cgactacagg aagcggtgag accacgaccg cggcaggcac gacggcaagc 6541 cccggcggtg ggcccatgat aggcagaact cctaagctta gtgagctggt tttaggtgaa 6601 actgctgaag cgcttagtct gcattgcgac gaagcattag agaatttaag tgatgatgat 6661 gaggaggatc atcaagatag acaggtgttc atagaaaggc cctatgcagt gtccgtgcca 6721 tgtaagcgct gtaggcaaac tatcagcttc gtctgcgtct gtgctccaga agccataaga 6781 accttgaatc gactgctatc cgcatcgctt tccctggtgt gcccggagtg ttgtaacctc 6841 ctcctcctcc tcctcatcga tgtcgacgta gttaactagc gtacgatcga ctgccaggca 6901 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 6961 cggccatcat ggccgcggtg atca //