LOCUS       62056_3765        7540 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        801..905
                     /label=AmpR promoter
     CDS             906..1763
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1937..2525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4861..5862)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     CDS             7506..7523
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
ORIGIN
        1 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
       61 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
      121 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
      181 ggtaacgccg ttccgcaccc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
      241 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
      301 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      361 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      421 catctccttg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
      481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgat atggtgcact ctcagtacaa
      541 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
      601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
      661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
      721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2761 gctgatttca ctttttgcat tctacaaact gcataactca tatgtaaatc gctccttttt
     2821 aggtggcaca aatgtgaggc attttcgctc tttccggcaa ccacttccaa gtaaagtata
     2881 acacactata ctttatattc ataaagtgtg tgctctgcga ggctgtcggc agtgccgacc
     2941 aaaaccataa aacctttaag acctttcttt tttttacgag aaaaaagaaa caaaaaaacc
     3001 tgccctctgc cacctcagca aaggggggtt ttgctctcgt gctcgtttaa aaatcagcaa
     3061 gggacaggta gtattttttg agaagatcac tcaaaaaatc tccaccttta aacccttgcc
     3121 aatttttatt ttgtccgttt tgtctagctt accgaaagcc agactcagca agaataaaat
     3181 ttttattgtc tttcggtttt ctagtgtaac ggacaaaacc actcaaaata aaaaagatac
     3241 aagagaggtc tctcgtatct tttattcagc aatcgcgccc gattgctgaa cagattaata
     3301 atagatttta gctttttatt tgttgaaaaa agctaatcaa attgttgtcg ggatcaatta
     3361 ctgcaaagtc tcgttcatcc caccactgat cttttaatga tgtattgggg tgcaaaatgc
     3421 ccaaaggctt aatatgttga tataattcat caattccctc tacttcaatg cggcaactag
     3481 cagtaccagc aataaacgac tccgcacctg tacaaaccgg tgaatcatta ctacgagagc
     3541 gccagcttca tcacttgcct cccatagatg aatccgaacc tcattacaca ttagaactgc
     3601 gaatccatct tcatggtgaa ccaaagtgaa acctagttta tcgcaataaa aacctatact
     3661 ctttttaata tccccgactg gcaatgccgg gatagactgt aacattctca cgcataaaat
     3721 cccctttcat tttctaatgt aaatctatta ccttattatt aattcaattc gctcataatt
     3781 aatccttttt cttattacgc aaaatggccc gatttaagca caccctttat tccgttaatg
     3841 cgccatgaca gccatgataa ttactaatac taggagaagt taataaatac gtaaccaaca
     3901 tgattaacaa ttattagagg tcatcgttca aaatggtatg cgttttgaca catccactat
     3961 atatccgtgt cgttctgtcc actcctgaat cccattccag aaattctcta gcgattccag
     4021 aagtttctca gagtcggaaa gttgaccaga cattacgaac tggcacagat ggtcataacc
     4081 tgaaggaaga tctgattgct taactgcttc agttaagacc gaagcgctcg tcgtataaca
     4141 gatgcgatga tgcagaccaa tcaacatggc acctgccatt gctacctgta cagtcaagga
     4201 tggtagaaat gttgtcggtc cttgcacacg aatattacgc catttgcctg catattcaaa
     4261 cagctcttct acgataaggg cacaaatcgc atcgtggaac gtttgggctt ctaccgattt
     4321 agcagtttga tacactttct ctaagtatcc acctgaatca taaatcggca aaatagagaa
     4381 aaattgacca tgtgtaagcg gccaatctga ttccacctga gatgcataat ctagtagaat
     4441 ctcttcgcta tcaaaattca cttccacctt ccactcaccg gttgtccatt catggctgaa
     4501 ctctgcttcc tctgttgaca tgacacacat catctcaata tccgaatagg gcccatcagt
     4561 ctgacgacca agagagccat aaacaccaat agccttaaca tcatccccat atttatccaa
     4621 tattcgttcc ttaatttcat gaacaatctt cattctttct tctctagtca ttattattgg
     4681 tccattcact attctcattc ccttttcaga taattttaga tttgcttttc taaataagaa
     4741 tatttggaga gcaccgttct tattcagcta ttaataactc gtcttcctaa gcatccttca
     4801 atccttttaa taacaattat agcatctaat cttcaacaaa ctggcccgtt tgttgaacta
     4861 ctctttaata aaataatttt tccgttccca attccacatt gcaataatag aaaatccatc
     4921 ttcatcggct ttttcgtcat catctgtatg aatcaaatcg ccttcttctg tgtcatcaag
     4981 gtttaatttt ttatgtattt cttttaacaa accaccatag gagattaacc ttttacggtg
     5041 taaaccttcc tccaaatcag acaaacgttt caaattcttt tcttcatcat cggtcataaa
     5101 atccgtatcc tttacaggat attttgcagt ttcgtcaatt gccgattgta tatccgattt
     5161 atatttattt ttcggtcgaa tcatttgaac ttttacattt ggatcatagt ctaatttcat
     5221 tgcctttttc caaaattgaa tccattgttt ttgattcacg tagttttctg tattcttaaa
     5281 ataagttggt tccacacata ccaatacatg catgtgctga ttataagaat tatctttatt
     5341 atttattgtc acttccgttg cacgcataaa accaacaaga tttttattaa tttttttata
     5401 ttgcatcatt cggcgaaatc cttgagccat atctgacaaa ctcttattta attcttcgcc
     5461 atcataaaca tttttaactg ttaatgtgag aaacaaccaa cgaactgttg gcttttgttt
     5521 aataacttca gcaacaacct tttgtgactg aatgccatgt ttcattgctc tcctccagtt
     5581 gcacattgga caaagcctgg atttacaaaa ccacactcga tacaactttc tttcgcctgt
     5641 ttcacgattt tgtttatact ctaatatttc agcacaatct tttactcttt cagccttttt
     5701 aaattcaaga atatgcagaa gttcaaagta atcaacatta gcgattttct tttctctcca
     5761 tggtctcact tttccacttt ttgtcttgtc cactaaaacc cttgattttt catctgaata
     5821 aatgctacta ttaggacaca taatattaaa agaaaccccc atctatttag ttatttgttt
     5881 agtcacttat aactttaaca gatggggttt ttctgtgcaa ccaattttaa gggttttcaa
     5941 tactttaaaa cacatacata ccaacacttc aacgcacctt tcagcaacta aaataaaaat
     6001 gacgttattt ctatatgtat caagataaga aagaacaagt tcaaaaccat caaaaaaaga
     6061 caccttttca ggtgcttttt ttattttata aactcattcc ctgatctcga cttcgttctt
     6121 tttttacctc tcggttatga gttagttcaa attcgttctt tttaggttct aaatcgtgtt
     6181 tttcttggaa ttgtgctgtt ttatccttta ccttgtctac aaacccctta aaaacgtttt
     6241 taaaggcttt taagccgtct gtacgttcct taaggaattc gagctcagct ttattgagtg
     6301 gatgattata ttccttttga taggtggtat gttttcgctt gaacttttaa atacagccat
     6361 tgaacatacg gttgatttaa taactgacaa acatcaccct cttgctaaag cggccaagga
     6421 cgctgccgcc ggggctgttt gcgtttttgc cgtgatttcg tgtattattg gtttacttat
     6481 ttttttgcca aagctgtaat ggctgaaaat tcttacattt attttacatt tttagaaatg
     6541 ggcgtgaaaa aaagcgcgcg attatgtaaa atataaagtg atagcggtac cattataggt
     6601 aagagaggaa tgtacacatg gcatacgaca gtcgttttga tgaatgggta cagaaactga
     6661 aagaggaaag ctttcaaaac aatacgtttg accgccgcaa atttattcaa ggagcgggga
     6721 agattgcagg actttctctt ggattaacga ttgcccagtc ggttggggcc tttgaagtaa
     6781 atgctgcgcc taacttcgcg acgggactcg acgacccggc gaagaaggag atcgccatgc
     6841 aactggtgtc gagcgccgag aactcctcgc tcgactggaa ggcccagtac cggtacatcg
     6901 aggacatcgg tgacggtcgc ggctacaccg cgggcatcat cggcttctgt tccggcaccg
     6961 gcgacatgct cgacctggtg gagctgtacg gcgagcgcag ccccgggaac gtcctcgcgc
     7021 cctatctgcc ggcgctgcgc cgggtggacg gctccgactc gcacgagggc ctcgaccccg
     7081 gcttcccgga cgactggcgc cgggccgccg accaggaccc gcagttccgc cgggcccagg
     7141 acgacgagcg ggaccgcgtg tacttcgacc cggccgtacg gcgcggcaag gaggacgggc
     7201 tgcggacgct ggggcagttc gcgtactacg acgcgatggt gatgcacggc gacggcggcg
     7261 gcctcggctt cggcagtatc cgcgagcgcg ccctcggccg ggcccgcccg ccggcccagg
     7321 gcggtgacga ggtcgcctac ctgcacgcct tcctcgacga gcgggtgtgg gccatgaagc
     7381 aggagcaggc gcacagcgac accagccggg tcgacacggc gcagcgcgtc ttcctgaacg
     7441 agggcaacct ggacctcgag ccgccgctgg actggcacgt gtacggggac gcctaccaca
     7501 tcggccacca ccaccaccac cactgaaagc ttgtattcta
//
