LOCUS 40924_6302 3959 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBG51, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3959) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE A Tn7-based device for calibrated heterologous gene expression in Pseudomonas putida JOURNAL ACS Synth Biol (2015) In press PUBMED 26133359 REFERENCE 2 (bases 1 to 3959) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (09-JUN-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3959) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (13-NOV-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 4 (bases 1 to 3959) TITLE Direct Submission REFERENCE 5 (bases 1 to 3959) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth Biol (2015) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (09-JUN-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (13-NOV-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain" COMMENT SGRef: number: 4; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## On Nov 13, 2015 this sequence version replaced KT192133.1. FEATURES Location/Qualifiers source 1..3959 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 9..49 /label=promoter 14d /note="promoter 14d" /regulatory_class="promoter" misc_feature 56..132 /label=BCD2 bicistronic linker /note="BCD2 bicistronic linker" RBS 124..132 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 151..864 /label=superfolder GFP /note="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" misc_feature 868..924 /label=MCS /note="pUC18/19 multiple cloning site" terminator 967..1061 /label=lambda t0 terminator /note="transcription terminator from phage lambda" misc_feature 1066..1264 /label=Tn7R transposase recognition site /note="Tn7R transposase recognition site" CDS 1374..2186 /label=KanR /note="aminoglycoside phosphotransferase" oriT 2345..2453 /label=oriT /note="incP origin of transfer" rep_origin 2472..2860 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" mobile_element 2872..3037 /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)" terminator complement(3051..3137) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(3206..3736) /label=GmR /note="gentamycin acetyltransferase" promoter complement(3925..3953) /label=Pc promoter /note="class 1 integron promoter" ORIGIN 1 ttaattaatc tacttgacat ccgacattcg cgactgtata ataagttgac ctagggccca 61 agttcactta aaaaggagat caacaatgaa agcaattttc gtactgaaac atcttaatca 121 tgctaaggag gttttctaat gatcatggga attcataaag gtgaagaact gttcaccggt 181 gttgttccga tcctggttga actggatggt gatgttaacg gccacaaatt ctctgttcgt 241 ggtgaaggtg aaggtgatgc aaccaacggt aaactgaccc tgaaattcat ctgcactacc 301 ggtaaactgc cggttccatg gccgactctg gtgactaccc tgacctatgg tgttcagtgt 361 ttttctcgtt acccggatca catgaagcag catgatttct tcaaatctgc aatgccggaa 421 ggttatgtac aggagcgcac catttctttc aaagacgatg gcacctacaa aacccgtgca 481 gaggttaaat ttgaaggtga tactctggtg aaccgtattg aactgaaagg cattgatttc 541 aaagaggacg gcaacatcct gggccacaaa ctggaatata acttcaactc ccataacgtt 601 tacatcaccg cagacaaaca gaagaacggt atcaaagcta acttcaaaat tcgccataac 661 gttgaagacg gtagcgtaca gctggcggac cactaccagc agaacactcc gatcggtgat 721 ggtccggttc tgctgccgga taaccactac ctgtccaccc agtctaaact gtccaaagac 781 ccgaacgaaa agcgcgacca catggtgctg ctggagttcg ttactgcagc aggtatcacg 841 cacggcatgg atgaactcta caaataagaa ttcgagctcg gtacccgggg atcctctaga 901 gtcgacctgc aggcatgcaa gcttgcggcc gcgtcgtgac tgggaaaacc ctggcgacta 961 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca 1021 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccagccacg cccctcttta 1081 atacgacggg caatttgcac ttcagaaaat gaagagtttg ctttagccat aacaaaagtc 1141 cagtatgctt tttcacagca taactggact gatttcagtt tacaactatt ctgtctagtt 1201 taagacttta ttgtcatagt ttagatctat tttgttcagt ttaagacttt attgtccgcc 1261 cacaatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 1321 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 1381 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc 1441 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc 1501 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga 1561 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga 1621 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg 1681 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg 1741 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca 1801 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg 1861 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc 1921 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata 1981 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg 2041 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc 2101 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat 2161 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcc ttttccgctg 2221 cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac 2281 gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc 2341 agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc 2401 cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccgtaggccg 2461 gccggcgcgc cgatctgaag atcagcagtt caacctgttg atagtacgta ctaagctctc 2521 atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa 2581 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg 2641 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct 2701 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ctttaaggtt 2761 taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta gagcctctca 2821 aagcaatttt gagtgacaca ggaacactta acggctgaca tggggcgcgc ctgtgggcgg 2881 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag 2941 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg 3001 acaaaatagt ttggaactag atttcactta tctggtttct agggcggcgg atttgtccta 3061 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga 3121 gcctttcgtt ttatttgatg ccgacgtcca atttaccgaa caactccgcg gccgggaagc 3181 cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca 3241 cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg 3301 cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga 3361 gcgcggtggc aatgccctgc ctccggtgct ctccggagac tgcgagatca tagatataga 3421 tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg 3481 cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt 3541 aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa 3601 gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga 3661 tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt 3721 tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc 3781 gcttgctgct tggatgcccg aggcataggc tgtacaaaaa aacagtcata acaagccatg 3841 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt 3901 gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt caagacgtc //