LOCUS       40924_6571        5682 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle expression vector pBJ3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Equbal MJ, Agarwal GP.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 5682)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5682)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (14-JUN-2010) Department of Biochemical Engineering "
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    complement(158..203)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             complement(207..239)
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             complement(243..260)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(270..287)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(306..328)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(343..367)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(368..386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             461..1117
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    1577..4327
                     /note="BL1 replicon derived from Corynebacterium glutamicum
                     plasmid pBL1 X03987 for replication in Corynebacterium 
                     glutamicum"
     misc_feature    4465..4607
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4793..5381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
      181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
      241 tggctgccgc gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg
      301 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      361 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcactac cgggcgtatt
      421 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
      481 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
      541 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
      601 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
      661 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
      721 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
      781 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
      841 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
      901 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
      961 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1021 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     1081 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     1141 gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga
     1201 aattcgaaag caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt
     1261 atgtctattg ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct
     1321 caaatgcctg aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga
     1381 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cgatctcggt agtgggatac
     1441 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
     1501 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1561 aagggcaatc agaacaacaa gacccatcat agtttgcccc cgcgacattg accataaatt
     1621 catcgcacaa aatatcgaac ggggtttatg ccgcttttag tgggtgcgaa gaatagtctg
     1681 ctcattaccc gcgaacaccg ccgcattcag atcacgctta gtagcgtccc catgagtagg
     1741 cagaaccgcg tccaagtcca catcatccat aacgatcatg cacggggtgg aatccacacc
     1801 cagacttgcc agcacctcat tagcgacacg ttgcgcagcg gccacgtcct tagccttatc
     1861 cacgcaatct agaacgtact gcctaaccgc gaaatcagac tgaatcagtt tccaatcatc
     1921 gggcttcacc aaagcaacag caacgcgggt tgattcgacc cgttccggtg cttccagacc
     1981 ggcgagcttg tacagttctt cttccatttc acgacgtaca tcagcgtcta tgtaatcaat
     2041 gcccaaagca cgcttagccc cacgtgacca ggacgaacgc aggtttttag aaccaacctc
     2101 atactcacgc caccgagcca ccaaaacagc gtccatatcc tcgccggcgt cgctttgatc
     2161 ggccaacata tccaacatct gaaacggcgt gtacgacccc ttagacgcgg ttttagtagc
     2221 ggagccagtc agttcctgag acatgccctt agcgaggtag gttgccattt tcgcagcgtc
     2281 tccaccccag gtagacacct gatcaagttt gaccccgtgc tcacgcagtg gcgcgtccat
     2341 accggcctta accacaccag cagaccagcg ggaaaacatg gaatcctcaa acgccttgag
     2401 ttcatcgtca gacagtggac gatccaagaa caacagcatg ttgcggtgca agtgccaacc
     2461 gttcgcccaa gagtctgtga cctcatagtc actataggtg tgctccaccc cgtaccgtgc
     2521 acgttctttc ttccactgag atgttttcac catcgaagag tacgcagtct taatacccgc
     2581 ttcaacctgc gcaaatgact gtgagcggtt gtgtcgaaca gtgcccacaa acatcatgag
     2641 cgcgccaccc gccgccaagt gattcttagt agcaatagcc agctcaatgc ggcgttcgcc
     2701 catgacttcc aattcagcca gaggtgaccc ccagcgagag tgagagtttt gcagaccctc
     2761 aaactgcgaa gcaccgttag acgaccagga caccgcaaca gcttcgtccc tgcgccacct
     2821 atggcacccc gccagagcct tactattggt gatcttgtac atgacgtttt gcctacgcca
     2881 cgccctagcg cgagtgacct tagaaccctc attgacctgc ggttccttag aggtgttcac
     2941 ttctatttca gtgttactca gtgttaccta gacccgatgt tgtgcggggt tgcgcagtgc
     3001 gagtttgtgc gggtgttgtg cccgttgtct tagctagtgc tatggttgtc aattgaaacc
     3061 ccttcgggtt atgtggcccc cgtgcatatg agttggtagc tcgcacgggg gtttgtcttg
     3121 tctagggact attaattttt agtggtgttt ggtggccgcc tagcttggct atgcgtgcca
     3181 gcttacccgt actcaatgtt aaagatttgc atcgacatgg gagggttacg tgtccgatac
     3241 ctaggggggg tatccgcgac taggtgcccc ggtgctcact gtctgtaccg gcggggcaag
     3301 ccccacaccc cgcatggaca gggtggctcc gccccctgca cccccagcaa tctgcatgta
     3361 catgttttac acattagcac gacatgactg catgtgcatg cactgcatgc agactaggta
     3421 aatatgagta tgtacgacta gtaacaggag cactgcacat aatgaatgag ttgcaggaca
     3481 atgtttgcta cgcatgcgca tgacatatcg caggaaagct actagagtct taaagcatgg
     3541 caaccaaggc acagctagaa cagcaactac aagaagctca acaggcacta caggcgcagc
     3601 aagcgcaggc acaagccacc atcgaagcac tagaagcgca ggcaaaggct aagcccgtcg
     3661 tggtcaccgc acgcgttcct ttggcactac gtgaggacat gaagcgcgca ggcatgcaga
     3721 acggtgaaaa cctccaagag ttcatgatcg ccgcgtttac cgagcggcta gaaaagctca
     3781 ccaccaccga caacgaggaa aacaatgtct aacccactag ttctctttgc ccaccgtgac
     3841 ccggtaaatg acgtgacgtt cgagtgcatt gagcacgcca cctacgacac actttcacac
     3901 gctaaagacc agatcaccgc ccaaatgcaa gccctagacg aagaagccgc cctactgccc
     3961 taatgggtgt ttcatgggtg tttccctagt gtttcatggt gttttcacct aagctaggga
     4021 attgcgcgag aagtctcgca aaaatcagca acccccggaa ccacacagtt cacgggggtt
     4081 cttctatgcc agaaatcaga aaggggaacc agtgaacgac cccgaatatt ggatcacagc
     4141 gcagcaggtc gccgcccgcg tagctctcac cccggccacc attaaaaagt gggcaaacga
     4201 gggaaaaatc accgcataca agatcggcaa gtccgtccga ttcaaagcat cagacgtaga
     4261 caagcttgcc acaccccgag acgcagccta gaagccattc taagcgacta tctagccccg
     4321 gttctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4381 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4441 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     4501 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
     4561 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     4621 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4681 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4741 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4801 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4861 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4921 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4981 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5041 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5101 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5161 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5221 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5281 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5341 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5401 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca
     5461 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     5521 gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     5581 aaatgggctc gtagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     5641 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca
//
