LOCUS       V029794                 6951 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029794
VERSION     V029794
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..4462
                     /label="FILIP1L(NM_001387850)"
                     /note="FILIP1L(NM_001387850)"
                     /gene="FILIP1L"
     polyA_signal    4505..4626
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5045..5633)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5833..6693)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    6798..6846
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    6860..6951
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgcgttc
      901 cagaggcagt gataccgagg gctcagccca aaagaaattt ccaagacata ctaaaggcca
      961 cagtttccaa gggcctaaaa acatgaagca tagacagcaa gacaaagact cccccagtga
     1021 gtcggatgta atacttccgt gtcccaaggc agagaagcca cacagtggta atggccacca
     1081 agcagaagac ctctcaagag atgacctgtt atttctcctc agcattctgg agggagaact
     1141 gcaggctcga gatgaggtca taggcatttt aaaggctgaa aaaatggacc tggctttgct
     1201 ggaagctcag tatgggtttg tcactccaaa aaaggtgtta gaggctctcc agagagatgc
     1261 ttttcaagcg aaatctaccc cttggcagga ggacatctat gagaaaccaa tgaatgagtt
     1321 ggacaaagtt gtggaaaaac ataaagaatc ttacagacga atcctgggac agcttttagt
     1381 ggcagaaaaa tcccgtaggc aaaccatatt ggagttggag gaagaaaaga gaaaacataa
     1441 agaatacatg gagaagagtg atgaattcat atgcctacta gaacaggaat gtgaaagatt
     1501 aaagaagcta attgatcaag aaatcaagtc tcaggaggag aaggagcaag aaaaggagaa
     1561 aagggtcacc accctgaaag aggagctgac caagctgaag tcttttgctt tgatggtggt
     1621 ggatgaacag caaaggctga cggcacagct cacccttcaa agacagaaaa tccaagagct
     1681 gaccacaaat gcaaaggaaa cacataccaa actagccctt gctgaagcca gagttcagga
     1741 ggaagagcag aaggcaacca gactagagaa ggaactgcaa acgcagacca caaagtttca
     1801 ccaagaccaa gacacaatta tggcgaagct caccaatgag gacagtcaaa atcgccagct
     1861 tcaacaaaag ctggcagcac tcagccggca gattgatgag ttagaagaga caaacaggtc
     1921 tttacgaaaa gcagaagagg agctgcaaga tataaaagaa aaaatcagta agggagaata
     1981 tggaaacgct ggtatcatgg ctgaagtgga agagctcagg aaacgtgtgc tagatatgga
     2041 agggaaagat gaagagctca taaaaatgga ggagcagtgc agagatctca ataagaggct
     2101 tgaaagggag acgttacaga gtaaagactt taaactagag gttgaaaaac tcagtaaaag
     2161 aattatggct ctggaaaagt tagaagacgc tttcaacaaa agcaaacaag aatgctactc
     2221 tctgaaatgc aatttagaaa aagaaaggat gaccacaaag cagttgtctc aagaactgga
     2281 gagtttaaaa gtaaggatca aagagctaga agccattgaa agtcggctag aaaagacaga
     2341 attcactcta aaagaggatt taactaaact gaaaacatta actgtgatgt ttgtagatga
     2401 acggaaaaca atgagtgaaa aattaaagaa aactgaagat aaattacaag ctgcttcttc
     2461 tcagcttcaa gtggagcaaa ataaagtaac aacagttact gagaagttaa ttgaggaaac
     2521 taaaagggcg ctcaagtcca aaaccgatgt agaagaaaag atgtacagcg taaccaagga
     2581 gagagatgat ttaaaaaaca aattgaaagc ggaagaagag aaaggaaatg atctcctgtc
     2641 aagagttaat atgttgaaaa ataggcttca atcattggaa gcaattgaga aagatttcct
     2701 aaaaaacaaa ttaaatcaag actctgggaa atccacaaca gcattacacc aagaaaacaa
     2761 taagattaag gagctctctc aagaagtgga aagactgaaa ctgaagctaa aggacatgaa
     2821 agccattgag gatgacctca tgaaaacaga agatgaatat gagactctag aacgaaggta
     2881 tgctaatgaa cgagacaaag ctcaattttt atctaaagag ctagaacatg ttaaaatgga
     2941 acttgctaag tacaagttag cagaaaagac agagaccagc catgaacaat ggcttttcaa
     3001 aaggcttcaa gaagaagaag ctaagtcagg gcacctctca agagaagtgg atgcattaaa
     3061 agagaaaatt catgaataca tggcaactga agacctaata tgtcacctcc agggagatca
     3121 ctcagtcctg caaaaaaaac taaatcaaca agaaaacagg aacagagatt taggaagaga
     3181 gattgaaaac ctcactaagg agttagagag gtaccggcat ttcagtaaga gcctcaggcc
     3241 tagtctcaat ggaagaagaa tttccgatcc tcaagtattt tctaaagaag ttcagacaga
     3301 agcagtagac aatgaaccac ctgattacaa gagcctcatt cctctggaac gtgcagtcat
     3361 caatggtcag ttatatgagg agagtgagaa tcaagacgag gaccctaatg atgagggatc
     3421 tgtgctgtcc ttcaaatgca gccagtctac tccatgtcct gttaacagaa agctatggat
     3481 tccctggatg aaatccaagg agggccatct tcagaatgga aaaatgcaaa ctaaacccaa
     3541 tgccaacttt gtgcaacctg gagatctagt cctaagccac acacctgggc agccacttca
     3601 tataaaggtt actccagacc atgtacaaaa cacagccact cttgaaatca caagtccaac
     3661 cacagagagt cctcactctt acacgagtac tgcagtgata ccgaactgtg gcacgccaaa
     3721 gcaaaggata accatcctcc aaaacgcctc cataacacca gtaaagtcca aaacctctac
     3781 cgaagacctc atgaatttag aacaaggcat gtccccaatt accatggcaa cctttgccag
     3841 agcacagacc ccagagtctt gtggttctct aactccagaa aggacaatgt cccctattca
     3901 ggttttggct gtgactggtt cagctagctc tcctgagcag ggacgctccc cagaaccaac
     3961 agaaatcagt gccaagcatg cgatattcag agtctcccca gaccggcagt catcatggca
     4021 gtttcagcgt tcaaacagca atagctcaag tgtgataact actgaggata ataaaatcca
     4081 cattcactta ggaagtcctt acatgcaagc tgtagccagc cctgtgagac ctgccagccc
     4141 ttcagcacca ctgcaggata accgaactca aggcttaatt aacggggcac taaacaaaac
     4201 aaccaataaa gtcaccagca gtattactat cacaccaaca gccacacctc ttcctcgaca
     4261 atcacaaatt acaatcaagg aggtatgcaa gcagagaatc ccagcacgga tccctaaacc
     4321 gaaatctaca ggcatcacca agatttccac taaagccccc acagtgccta tggataagcc
     4381 tatttaccaa aatggctgtg ggaagctaca catcgtgaga actgtcacct ccaacacctt
     4441 cctccacgtg ggagggagaa ggtaatctag agtcggggcg gccggccgct tcgagcagac
     4501 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4561 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4621 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4681 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc
     4741 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     4801 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     4861 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4921 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4981 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5041 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5101 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5161 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5221 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5281 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5341 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5401 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5461 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5521 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5581 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5641 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5701 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5761 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa
     5821 ccactgcagt ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt
     5881 tcgttcgtcc atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt
     5941 accatcaggc cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt
     6001 gtcagcaatg aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc
     6061 cgcctccatc cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag
     6121 tagtttccga agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg
     6181 tatggcttcg ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt
     6241 atgaagaaat gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc
     6301 ggtgttgtcg ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt
     6361 aagatgcttt tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg
     6421 gcgaccaagc tgctcttgcc cggcgtctat acgggacaac accgcgccac atagcagtac
     6481 tttgaaagtg ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc
     6541 gctattgaga tccagttcga tatagcccac tcttgcaccc agttgatctt cagcatcttt
     6601 tactttcacc agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg
     6661 aatgagtgcg acacgaaaat gttggatgct catactcgtc ctttttcaat attattgaag
     6721 catttatcag ggttactagt acgtctctca aggataagta agtaatatta aggtacggga
     6781 ggtattggac aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     6841 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     6901 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc t
//
