LOCUS       pBiT2.1-HMGB1(human)    4026 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029781
VERSION     V029781
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..1537
                     /label="HMGB1(NM_002128)"
                     /note="HMGB1(NM_002128)"
                     /gene="HMGB1"
     polyA_signal    1580..1701
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2120..2708)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2908..3768)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    3873..3921
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    3935..4026
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatgggcaa
      901 aggagatcct aagaagccga gaggcaaaat gtcatcatat gcattttttg tgcaaacttg
      961 tcgggaggag cataagaaga agcacccaga tgcttcagtc aacttctcag agttttctaa
     1021 gaagtgctca gagaggtgga agaccatgtc tgctaaagag aaaggaaaat ttgaagatat
     1081 ggcaaaagcg gacaaggccc gttatgaaag agaaatgaaa acctatatcc ctcccaaagg
     1141 ggagacaaaa aagaagttca aggatcccaa tgcacccaag aggcctcctt cggccttctt
     1201 cctcttctgc tctgagtatc gcccaaaaat caaaggagaa catcctggcc tgtccattgg
     1261 tgatgttgcg aagaaactgg gagagatgtg gaataacact gctgcagatg acaagcagcc
     1321 ttatgaaaag aaggctgcga agctgaagga aaaatatgaa aaggatattg ctgcatatcg
     1381 agctaaagga aagcctgatg cagcaaaaaa gggagttgtc aaggctgaaa aaagcaagaa
     1441 aaagaaggaa gaggaggaag atgaggaaga tgaagaggat gaggaggagg aggaagatga
     1501 agaagatgaa gatgaagaag aagatgatga tgatgaataa tctagagtcg gggcggccgg
     1561 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1621 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1681 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     1741 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     1801 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1861 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1921 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1981 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2041 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2101 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2221 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2281 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2341 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2401 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2461 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2521 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2581 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2641 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2701 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2761 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2821 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     2881 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     2941 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3001 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3061 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3121 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     3181 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     3241 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     3301 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     3361 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     3421 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     3481 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     3541 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     3601 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     3661 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     3721 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     3781 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     3841 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     3901 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     3961 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4021 ttctct
//
