LOCUS       pBiT2.1-RPS11(human)    3855 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029674
VERSION     V029674
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..1366
                     /label="RPS11(NM_001015)"
                     /note="RPS11(NM_001015)"
                     /gene="RPS11"
     polyA_signal    1409..1530
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(1949..2537)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2737..3597)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    3702..3750
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    3764..3855
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatggcgga
      901 cattcagact gagcgtgcct accaaaagca gccgaccatc tttcaaaaca agaagagggt
      961 cctgctggga gaaactggca aggagaagct cccgcggtac tacaagaaca tcggtctggg
     1021 cttcaagaca cccaaggagg ctattgaggg cacctacatt gacaagaaat gccccttcac
     1081 tggtaatgtg tccattcgag ggcggatcct ctctggcgtg gtgaccaaga tgaagatgca
     1141 gaggaccatt gtcatccgcc gagactatct gcactacatc cgcaagtaca accgcttcga
     1201 gaagcgccac aagaacatgt ctgtacacct gtccccctgc ttcagggacg tccagatcgg
     1261 tgacatcgtc acagtgggcg agtgccggcc tctgagcaag acagtgcgct tcaacgtgct
     1321 caaggtcacc aaggctgccg gcaccaagaa gcagttccag aagttctgat ctagagtcgg
     1381 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     1441 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     1501 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     1561 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     1621 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     1681 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     1741 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2701 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     2761 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     2821 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     2881 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     2941 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     3001 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     3061 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     3121 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     3181 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     3241 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     3301 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     3361 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     3421 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     3481 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     3541 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     3601 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     3661 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     3721 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     3781 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     3841 ccagaacatt tctct
//
