LOCUS pBiT2.1-SLTM(human) 6483 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030037 VERSION V030037 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 28..779 /note="herpes simplex virus thymidine kinase promoter" /label="HSV TK promoter" regulatory 809..818 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 893..3994 /label="SLTM(NM_024755)" /note="SLTM(NM_024755)" /gene="SLTM" polyA_signal 4037..4158 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4577..5165) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5365..6225) /codon_start=1 /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" polyA_signal 6330..6378 /note="synthetic polyadenylation signal" /label="poly(A) signal" misc_feature 6392..6483 /note="RNA polymerase II transcriptional pause signal from the human α2 globin gene" /label="pause site" ORIGIN 1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa 61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg 121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc 181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg 241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac 301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc 361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc 421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg 481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg 541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg 601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg 661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg 721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa 781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga 841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatggctgc 901 cgctaccggt gcggtggcag cctcggccgc ctcgggtcag gcggaaggta aaaagatcac 961 cgatctgcgg gtcatcgatc tgaagtccga gctgaagcgg cggaacttag acatcaccgg 1021 agtcaagacc gtgctcatct cccgactcaa gcaggctatt gaagaggaag gaggcgatcc 1081 agataatatt gaattaactg tttcaactga tactccaaac aagaaaccaa ctaaaggcaa 1141 aggtaaaaaa catgaagcag atgagttgag tggagatgct tctgtggaag atgatgcttt 1201 tatcaaggac tgtgaattgg agaatcaaga ggcacatgag caagatggaa atgatgaact 1261 aaaggactct gaagaatttg gtgaaaatga agaagaaaat gtgcattcca aggagttact 1321 ctctgcagaa gaaaacaaga gagctcatga attaatagag gcagaaggaa tagaagatat 1381 agaaaaagag gacatcgaaa gtcaggaaat tgaagctcaa gaaggtgaag atgatacctt 1441 tctaacagcc caagatggtg aggaagaaga aaatgagaaa gatatagcag gttctggtga 1501 tggtacacaa gaagtatcta aacctcttcc ttcagaaggg agcctagctg aggctgatca 1561 cacagctcat gaagagatgg aagctcatac gactgtgaaa gaagctgagg atgacaacat 1621 ctcggtcaca atccaggctg aagatgccat cactctggat tttgatggtg atgacctcct 1681 agaaacaggt aaaaatgtga aaattacaga ttctgaagca agtaagccaa aagatgggca 1741 ggacgccatt gcacagagcc cggagaagga aagcaaggat tatgagatga atgcgaacca 1801 taaagatggt aagaaggaag actgcgtgaa gggtgaccct gtcgagaagg aagccagaga 1861 aagttctaag aaagcagaat ctggagacaa agaaaaggat actttgaaga aagggccctc 1921 gtctactggg gcctctggtc aagcaaagag ctcttcaaag gaatctaaag acagcaagac 1981 atcatctaaa gatgacaaag gaagtacaag tagtactagt ggtagcagtg gaagctcaac 2041 taaaaatatc tgggttagtg gactttcatc taataccaaa gctgctgatt tgaagaacct 2101 ctttggcaaa tatggaaagg ttctgagtgc aaaagtagtt acaaatgctc gaagtcctgg 2161 ggcaaaatgc tatggcattg taactatgtc ttcaagcaca gaggtgtcca ggtgtattgc 2221 acatcttcat cgcactgagc tgcatggaca gctgatttct gttgaaaaag taaaaggtga 2281 tccctctaag aaagaaatga agaaagaaaa tgatgaaaag agtagttcaa gaagttctgg 2341 agataaaaaa aatacgagtg atagaagtag caagacacaa gcctctgtca aaaaagaaga 2401 gaaaagatcg tctgagaaat ctgaaaaaaa agaaagcaag gatactaaga aaatagaagg 2461 taaagatgag aagaatgata atggagcaag tggccaaaca tcagaatcga ttaaaaaaag 2521 tgaagaaaag aagcgaataa gttccaagag tccaggacat atggtaatac tagaccaaac 2581 taaaggagat cattgtagac catcaagaag aggaagatat gagaaaattc atggaagaag 2641 taaggaaaag gagagagcta gtctagataa aaaaagagat aaagactaca gaaggaaaga 2701 gatcttgcct tttgaaaaga tgaaggaaca aaggttgaga gaacatttag ttcgttttga 2761 aaggctgcga cgagcaatgg aacttcgaag acgaagagag attgcagaga gagagcgtcg 2821 agagcgagaa cgcattagaa taattcgtga acgggaagaa cgggaacgct tacagagaga 2881 gagagagcgc ctagaaattg aaaggcaaaa actagagaga gagagaatgg aacgcgaacg 2941 cttggaaagg gaacgcattc gtattgaaca ggaacgtcgt aaggaagctg aacggattgc 3001 tcgagaaaga gaggaactca gaaggcaaca acagcagctt cgttatgaac aagaaaaaag 3061 gaattccttg aaacgcccac gtgatgtaga tcataggcga gatgatcctt actggagcga 3121 gaataaaaag ttgtctctag atacagatgc acgatttggc catggatccg actactctcg 3181 ccaacagaac agatttaatg actttgatca ccgagagagg ggcaggtttc ctgagagttc 3241 agcagtacag tcttcatctt ttgaaaggcg ggatcgcttt gttggtcaaa gtgaggggaa 3301 aaaagcacga cctactgcac gaagggaaga tccaagcttc gaaagatatc ccaaaaattt 3361 cagtgactcc agaagaaatg agcctccacc accaagaaat gaacttagag aatcagacag 3421 gcgagaagta cgaggggagc gagacgaaag gagaacggtg attattcatg acaggcctga 3481 tatcactcat cctagacatc ctcgagaggc agggcccaat ccttccagac ccaccagctg 3541 gaaaagtgaa ggaagcatgt ccactgacaa acgggaaaca agagttgaaa ggccagaacg 3601 atctgggaga gaagtatcag ggcacagtgt gagaggcgct ccccctggga atcgtagcag 3661 cgcttcgggg tacgggagca gagagggaga cagaggagtc atcacagacc gaggaggtgg 3721 atcacagcac tatcctgagg agcgacatgt ggttgaacgc catggacggg acacaagcgg 3781 accaaggaaa gagtggcatg gtccaccctc tcaagggcct agctatcatg atacgaggcg 3841 aatgggtgac ggccgggcag gagcaggcat gataacccaa cattcaagta acgcatcccc 3901 aattaataga attgtacaaa tcagtggcaa ttccatgcca agaggaagtg gctccggatt 3961 taagccattt aagggtggac ctccgcgacg attctgatct agagtcgggg cggccggccg 4021 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 4081 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 4141 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 4201 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag 4261 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 4321 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 4381 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 5041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc 5341 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc 5401 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat 5461 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc 5521 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc 5581 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag 5641 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg 5701 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg 5761 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag 5821 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt 5881 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga 5941 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc 6001 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc 6061 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc 6121 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc 6181 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca 6241 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat 6301 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt 6361 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga 6421 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc 6481 tct //