LOCUS       pBiT2.1-SLTM(human)     6483 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030037
VERSION     V030037
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        28..779
                     /note="herpes simplex virus thymidine kinase promoter"
                     /label="HSV TK promoter"
     regulatory      809..818
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             893..3994
                     /label="SLTM(NM_024755)"
                     /note="SLTM(NM_024755)"
                     /gene="SLTM"
     polyA_signal    4037..4158
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4577..5165)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5365..6225)
                     /codon_start=1
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     polyA_signal    6330..6378
                     /note="synthetic polyadenylation signal"
                     /label="poly(A) signal"
     misc_feature    6392..6483
                     /note="RNA polymerase II transcriptional pause signal from
                     the human&nbsp;α2 globin gene"
                     /label="pause site"
ORIGIN
        1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
       61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
      121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
      181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
      241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
      301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
      361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
      421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
      481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
      541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
      601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
      661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
      721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
      781 agcttggcaa tccggtactg ttggtaaagc caccatggtg accggctacc ggctgttcga
      841 ggagattctc gggagttccg gtggtggcgg gagcggaggt ggaggctcga ggatggctgc
      901 cgctaccggt gcggtggcag cctcggccgc ctcgggtcag gcggaaggta aaaagatcac
      961 cgatctgcgg gtcatcgatc tgaagtccga gctgaagcgg cggaacttag acatcaccgg
     1021 agtcaagacc gtgctcatct cccgactcaa gcaggctatt gaagaggaag gaggcgatcc
     1081 agataatatt gaattaactg tttcaactga tactccaaac aagaaaccaa ctaaaggcaa
     1141 aggtaaaaaa catgaagcag atgagttgag tggagatgct tctgtggaag atgatgcttt
     1201 tatcaaggac tgtgaattgg agaatcaaga ggcacatgag caagatggaa atgatgaact
     1261 aaaggactct gaagaatttg gtgaaaatga agaagaaaat gtgcattcca aggagttact
     1321 ctctgcagaa gaaaacaaga gagctcatga attaatagag gcagaaggaa tagaagatat
     1381 agaaaaagag gacatcgaaa gtcaggaaat tgaagctcaa gaaggtgaag atgatacctt
     1441 tctaacagcc caagatggtg aggaagaaga aaatgagaaa gatatagcag gttctggtga
     1501 tggtacacaa gaagtatcta aacctcttcc ttcagaaggg agcctagctg aggctgatca
     1561 cacagctcat gaagagatgg aagctcatac gactgtgaaa gaagctgagg atgacaacat
     1621 ctcggtcaca atccaggctg aagatgccat cactctggat tttgatggtg atgacctcct
     1681 agaaacaggt aaaaatgtga aaattacaga ttctgaagca agtaagccaa aagatgggca
     1741 ggacgccatt gcacagagcc cggagaagga aagcaaggat tatgagatga atgcgaacca
     1801 taaagatggt aagaaggaag actgcgtgaa gggtgaccct gtcgagaagg aagccagaga
     1861 aagttctaag aaagcagaat ctggagacaa agaaaaggat actttgaaga aagggccctc
     1921 gtctactggg gcctctggtc aagcaaagag ctcttcaaag gaatctaaag acagcaagac
     1981 atcatctaaa gatgacaaag gaagtacaag tagtactagt ggtagcagtg gaagctcaac
     2041 taaaaatatc tgggttagtg gactttcatc taataccaaa gctgctgatt tgaagaacct
     2101 ctttggcaaa tatggaaagg ttctgagtgc aaaagtagtt acaaatgctc gaagtcctgg
     2161 ggcaaaatgc tatggcattg taactatgtc ttcaagcaca gaggtgtcca ggtgtattgc
     2221 acatcttcat cgcactgagc tgcatggaca gctgatttct gttgaaaaag taaaaggtga
     2281 tccctctaag aaagaaatga agaaagaaaa tgatgaaaag agtagttcaa gaagttctgg
     2341 agataaaaaa aatacgagtg atagaagtag caagacacaa gcctctgtca aaaaagaaga
     2401 gaaaagatcg tctgagaaat ctgaaaaaaa agaaagcaag gatactaaga aaatagaagg
     2461 taaagatgag aagaatgata atggagcaag tggccaaaca tcagaatcga ttaaaaaaag
     2521 tgaagaaaag aagcgaataa gttccaagag tccaggacat atggtaatac tagaccaaac
     2581 taaaggagat cattgtagac catcaagaag aggaagatat gagaaaattc atggaagaag
     2641 taaggaaaag gagagagcta gtctagataa aaaaagagat aaagactaca gaaggaaaga
     2701 gatcttgcct tttgaaaaga tgaaggaaca aaggttgaga gaacatttag ttcgttttga
     2761 aaggctgcga cgagcaatgg aacttcgaag acgaagagag attgcagaga gagagcgtcg
     2821 agagcgagaa cgcattagaa taattcgtga acgggaagaa cgggaacgct tacagagaga
     2881 gagagagcgc ctagaaattg aaaggcaaaa actagagaga gagagaatgg aacgcgaacg
     2941 cttggaaagg gaacgcattc gtattgaaca ggaacgtcgt aaggaagctg aacggattgc
     3001 tcgagaaaga gaggaactca gaaggcaaca acagcagctt cgttatgaac aagaaaaaag
     3061 gaattccttg aaacgcccac gtgatgtaga tcataggcga gatgatcctt actggagcga
     3121 gaataaaaag ttgtctctag atacagatgc acgatttggc catggatccg actactctcg
     3181 ccaacagaac agatttaatg actttgatca ccgagagagg ggcaggtttc ctgagagttc
     3241 agcagtacag tcttcatctt ttgaaaggcg ggatcgcttt gttggtcaaa gtgaggggaa
     3301 aaaagcacga cctactgcac gaagggaaga tccaagcttc gaaagatatc ccaaaaattt
     3361 cagtgactcc agaagaaatg agcctccacc accaagaaat gaacttagag aatcagacag
     3421 gcgagaagta cgaggggagc gagacgaaag gagaacggtg attattcatg acaggcctga
     3481 tatcactcat cctagacatc ctcgagaggc agggcccaat ccttccagac ccaccagctg
     3541 gaaaagtgaa ggaagcatgt ccactgacaa acgggaaaca agagttgaaa ggccagaacg
     3601 atctgggaga gaagtatcag ggcacagtgt gagaggcgct ccccctggga atcgtagcag
     3661 cgcttcgggg tacgggagca gagagggaga cagaggagtc atcacagacc gaggaggtgg
     3721 atcacagcac tatcctgagg agcgacatgt ggttgaacgc catggacggg acacaagcgg
     3781 accaaggaaa gagtggcatg gtccaccctc tcaagggcct agctatcatg atacgaggcg
     3841 aatgggtgac ggccgggcag gagcaggcat gataacccaa cattcaagta acgcatcccc
     3901 aattaataga attgtacaaa tcagtggcaa ttccatgcca agaggaagtg gctccggatt
     3961 taagccattt aagggtggac ctccgcgacg attctgatct agagtcgggg cggccggccg
     4021 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4081 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4141 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4201 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     4261 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     4321 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     4381 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4981 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc
     5341 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc
     5401 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat
     5461 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc
     5521 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc
     5581 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag
     5641 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg
     5701 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg
     5761 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag
     5821 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt
     5881 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga
     5941 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc
     6001 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc
     6061 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc
     6121 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc
     6181 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca
     6241 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat
     6301 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt
     6361 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
     6421 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     6481 tct
//
