LOCUS       40924_8346        5819 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAG-hUNRIP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             43..1092
                     /gene="STRAP"
                     /label=STRAP
                     /note="Serine-threonine kinase receptor-associated protein
                     from Homo sapiens. Accession#: Q9Y3F4"
     polyA_signal    1172..1227
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1588..1604)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1612..1628)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1636..1666)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1680..1701)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1759..1955
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1961..2095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2333..2921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3095..3952)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3953..4057)
                     /label=AmpR promoter
     enhancer        4092..4471
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4092..4469
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4473..4750
                     /label=chicken beta-actin promoter
     intron          4751..5768
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggcaat gagacagacg
       61 ccgctcacct gctctggcca cacgcgaccc gtggttgatt tggccttcag tggcatcacg
      121 ccttatgggt atttcttaat cagcgcttgc aaagatggta aacctatgct acgccaggga
      181 gatacaggag actggattgg aacatttttg ggtcataaag gtgctgtttg gggtgcaaca
      241 ctgaataagg atgccaccaa agcagctaca gcagctgcag atttcacagc caaagtgtgg
      301 gatgctgtct caggagatga attgatgacc ctggctcata aacacattgt caagactgtg
      361 gatttcacgc aggatagtaa ttatttgtta accgggggac aggataaact gttacgcata
      421 tatgacttga acaaacctga agcagaacct aaggaaatta gtggtcatac ttctggtata
      481 aaaaaagctc tgtggtgcag tgaggataaa cagattcttt ctgctgatga caaaactgtt
      541 cgactttggg atcatgctac tatgacagaa gtgaaatctc taaattttaa tatgtctgtt
      601 agtagtatgg aatatattcc tgagggagag attttggtta taacttatgg acgatctatt
      661 gcttttcata gtgcagtaag tttggaccca attaaatcct ttgaagctcc tgcaaccatc
      721 aattctgcat ctcttcatcc tgagaaagaa tttcttgttg caggcggtga agattttaaa
      781 ctttataagt atgattataa tagtggagaa gaattagaat cctacaaggg acactttggt
      841 cctattcact gtgtgagatt tagtcctgat ggagaactct atgccagtgg ttcagaagat
      901 ggaacattga gactatggca aactgtggta ggaaaaacgt atggcctttg gaaatgtgtg
      961 cttcctgaag aagatagtgg tgagctggca aagccaaaga ttggttttcc agagacaaca
     1021 gaagaggagc tagaagaaat tgcttcagag aattcagagt gcatctttcc ttcagctcct
     1081 gatgttaagg cctgagtcga cgaagatctt tttccctctg ccaaaaatta tggggacatc
     1141 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     1201 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     1261 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     1321 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     1381 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     1441 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     1501 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     1561 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1621 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa
     1681 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1741 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     2101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2221 tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag
     2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4081 ctgggtcgat cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     4141 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     4201 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     4261 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     4321 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     4381 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     4441 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     4501 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     4561 ttgtgcagcg atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg
     4621 cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct
     4681 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     4741 gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc
     4801 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     4861 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     4921 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     4981 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     5041 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     5101 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     5161 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     5221 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     5281 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     5341 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc
     5401 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     5461 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     5521 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     5581 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     5641 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     5701 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     5761 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa
//
