LOCUS       V021478                 7877 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021478
VERSION     V021478
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             16..3138
                     /label="IPO9(NM_018085)"
                     /note="IPO9(NM_018085)"
                     /gene="IPO9"
     CDS             3139..3162
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    3234..3289
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(3650..3666)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3674..3690
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3698..3728)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3742..3763
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        3822..4017
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    4023..4157
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4395..4983)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5154..6014)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6015..6119)
                     /gene="bla"
                     /label="AmpR promoter"
     enhancer        6150..6529
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        6531..6808
                     /label="chicken β-actin promoter"
     intron          6809..7826
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
ORIGIN
        1 ttcctcgagg ccaccatggc ggcggcggcg gcagctggtg cggcctccgg gctgccgggt
       61 ccagtggcac aaggattaaa ggaagcgtta gtggatacgc tcaccgggat cctatcccca
      121 gtacaggagg tgcgggcggc tgctgaagaa cagattaagg tgctggaggt gacggaggaa
      181 tttggtgttc acttggcaga actgactgta gatccccagg gggcactggc aatccgtcag
      241 ctggcatcag tcatcttgaa acaatatgtg gagactcact ggtgtgccca atcagagaaa
      301 tttaggcctc ctgaaactac agaaagggca aaaattgtta tccgggagct attgcctaat
      361 gggttgagag aatcgataag caaagtgcgc tccagtgtgg cctatgcagt gtcagccatt
      421 gcccactggg actggcctga agcttggccc caactcttca acctgctcat ggagatgttg
      481 gtgagcggag acttaaatgc cgtccatgga gccatgcgtg tgctgacaga attcactcgt
      541 gaagtaacag acacacagat gccacttgtt gctcctgtca ttctcccaga gatgtataag
      601 atcttcacca tggctgaggt gtatggtatt cgaacccgtt cccgagccgt ggagattttt
      661 accacttgtg cccatatgat ctgtaacatg gaggagctgg aaaagggtgc agccaaagtc
      721 ctgatctttc ccgtggtaca gcagttcaca gaggcctttg ttcaggccct ccagatacca
      781 gatggcccca catctgacag tgggtttaag atggaggtcc taaaggcagt gacagcccta
      841 gtgaaaaact tcccaaagca catggtgtcc tccatgcagc agattctgcc tattgtttgg
      901 aacaccctaa ccgagagtgc agctttttat gtgaggacag aagtaaatta cacagaagaa
      961 gtagaagatc ctgtggattc tgatggtgaa gtcctgggct ttgaaaatct cgtctttagc
     1021 atttttgaat ttgtccatgc tctactagaa aatagcaaat tcaaaagcac tgttaagaaa
     1081 gccttgcctg aattgattta ttatattatc ctgtacatgc aaatcactga ggagcagatt
     1141 aaagtatgga cagccaaccc ccaacaattt gtagaagatg aagatgatga tacattctcc
     1201 tatactgtta gaatagcagc tcaagacttg ttgctggctg tggccacaga tttccagaat
     1261 gaaagtgcag cagccctggc tgctgcagcc actcgacatt tacaagaagc tgagcaaacc
     1321 aaaaacagtg gcactgagca ctggtggaag atccatgagg catgcatgct tgccctaggc
     1381 tcagtgaagg ccatcatcac tgacagtgtg aaaaatggca ggattcattt tgacatgcat
     1441 gggttcctga ccaatgtcat ccttgcagac ctcaacctct cagtgtctcc tttcctcttg
     1501 ggccgggcac tttgggctgc cagtcggttc actgttgcta tgtcccctga actgatccag
     1561 cagttcctac aggcaacagt tagtggtctt cacgagacac agcccccatc agttcgaatt
     1621 tctgcagtga gagccatctg gggttattgt gaccaactga aagtctcaga gagtacccac
     1681 gtgctccagc ccttcctccc cagcatcctt gatggcttaa ttcacctagc agcccagttc
     1741 agctcagagg tcctcaacct ggtgatggag accctgtgca tcgtttgtac agtagacccc
     1801 gaattcacag caagcatgga aagcaaaatc tgccccttca ccatcgccat tttcctaaag
     1861 tacagtaatg atcccgtcgt cgcctcactg gctcaggaca tcttcaagga gctgtcccag
     1921 attgaagcct gtcagggccc aatgcaaatg aggctgattc ccactctggt cagcataatg
     1981 caggccccag cagacaagat tcctgcaggg ctttgtgcga cagccattga tatcctgaca
     2041 acagtagtac gaaatacaaa gcctcccctt tcccagcttc tcatctgcca agctttccct
     2101 gctgtggcac agtgtaccct tcacacagat gacaatgcca ccatgcagaa tggcggagag
     2161 tgcttgcggg cctatgtgtc agtgaccctg gaacaagtag cccagtggca tgatgagcag
     2221 ggccacaatg gactgtggta tgtgatgcaa gtggtgagcc agctcctgga cccccgcacc
     2281 tcagagttca ctgcggcctt tgtgggccgc cttgtttcca ccctcatctc caaggcaggg
     2341 cgggaactcg gggagaatct agaccagatt cttcgtgcca tcctcagtaa gatgcagcag
     2401 gcagagacgc tcagtgtcat gcagtccctg atcatggtgt tcgctcatct ggtgcacact
     2461 cagctagaac ctctcttgga gttcctgtgt agcctcccag gacctactgg caaacctgct
     2521 ctagagtttg tgatggctga gtggacaagc cgacagcacc tgttctatgg acagtatgaa
     2581 ggcaaagtca gctctgtggc actctgtaag ctgctccagc atggcatcaa tgcagatgac
     2641 aaacggctac aggatatccg tgtgaaggga gaggagatct acagcatgga tgagggcatc
     2701 cgcacccgct ctaagtcagc caaaaaccca gaacgctgga caaacattcc tttgctggtc
     2761 aagatcctaa agctgatcat caacgagctc tccaacgtca tggaggctaa tgccgctcgc
     2821 caggccactc ctgcagagtg gagtcaagat gactccaatg atatgtggga ggaccaggag
     2881 gaggaagagg aggaggagga ggatggttta gctggccaac ttttatctga cattcttgct
     2941 acaagtaaat atgaggagga ttactacgag gatgatgagg aagatgaccc tgatgccctg
     3001 aaggatcctc tctatcagat tgatctgcag gcatatctca cagatttcct ctgccagttt
     3061 gctcagcagc cctgctacat aatgttttca ggccacctta atgacaatga gaggcgagtt
     3121 ctacagacca tcggcatcga ttacaaggac gacgatgaca agtagagatc tttttccctc
     3181 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     3241 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     3301 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     3361 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     3421 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     3481 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     3541 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     3601 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     3661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3721 agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3781 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     3841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4021 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4081 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4141 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat
     4321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4801 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5041 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5101 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5161 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5221 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     5281 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5341 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5401 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5461 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5521 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5581 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5641 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5701 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5761 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5821 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5881 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5941 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6001 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6061 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6121 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta
     6181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     6241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     6541 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     6601 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc
     6661 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc
     6721 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     6781 ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc
     6841 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     6901 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     6961 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     7021 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     7081 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     7141 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     7201 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     7261 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     7321 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     7381 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     7441 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     7501 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     7561 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     7621 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     7681 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     7741 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     7801 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     7861 atcattttgg caaagaa
//
