LOCUS       V038508                11017 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038508
VERSION     V038508
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1738..1764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1771..7989
                     /label="KAT6B(NM_012330)"
                     /note="KAT6B(NM_012330)"
                     /gene="KAT6B"
     polyA_signal    8104..8159
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(8520..8536)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8544..8560
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8568..8598)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8613..8634
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        8693..8888
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    8894..9028
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(9266..9854)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10025..10885)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10886..10990)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac
     1741 ccatacgatg ttccagatta cgctgaattc atggtaaaac ttgcaaaccc actttataca
     1801 gagtggattc ttgaagctat acagaaaata aaaaagcaaa agcaaaggcc ctctgaagag
     1861 agaatctgcc atgcggtcag tacttcccat gggttggata agaagacagt ctctgaacag
     1921 ctggaactca gtgttcagga tggctcagtt ctcaaagtca ccaacaaagg ccttgcctcc
     1981 tataaggacc cagacaaccc tgggcgcttt tcatcagtta aaccaggcac ttttcctaag
     2041 tcagccaagg ggtctagagg atcatgtaat gatctccgca atgtggattg gaataaactt
     2101 ttaaggagag caattgaagg acttgaggag ccgaatggct cctccctgaa gaacatagag
     2161 aagtatctca gaagtcaaag tgatctcaca agcaccacca acaacccagc ctttcagcag
     2221 cggctgcgac tgggggccaa acgcgctgtg aataatggga ggttactgaa agacggaccg
     2281 cagtacaggg tcaattatgg gagcttagat ggcaaagggg cacctcagta tcccagtgca
     2341 ttcccatcct cgctcccacc tgtcagcctt ctaccccatg agaaagacca gccccgtgct
     2401 gatcccattc caatatgtag cttctgtttg gggactaaag aatcaaatcg tgaaaagaaa
     2461 ccagaagaac tcctctcttg tgcagattgt ggcagtagtg gacacccatc ctgtttgaaa
     2521 ttttgtcctg aattaacaac aaatgtaaag gccttaaggt ggcagtgcat cgaatgcaag
     2581 acatgcagtg cctgtagagt ccaaggcaga aatgctgata atatgctttt ttgtgattcc
     2641 tgtgatagag gatttcatat ggaatgctgt gacccaccac tttccagaat gccaaaaggg
     2701 atgtggattt gccaagtctg cagaccaaag aaaaagggaa gaaaactact tcatgagaaa
     2761 gctgcacaaa taaaacgacg atatgcaaaa cccattggac gaccgaaaaa taaattaaag
     2821 caacgattgt tgtctgtaac cagtgatgaa ggatccatga atgcattcac aggaaggggg
     2881 tcacctggta ggggtcaaaa gactaaagtc tgtaccacac cttcatctgg tcatgctgca
     2941 tctgggaagg actcaagcag cagattggct gttacagacc ccactcggcc tggtgccacc
     3001 accaaaatca ccaccacctc cacctacatt tctgcctcta cacttaaagt taacaagaaa
     3061 accaaagggc tcattgatgg ccttactaag ttttttacac catcacctga tggtcgcaga
     3121 tcacgaggtg aaattataga cttttcaaag cactatcgtc caaggaaaaa ggtctctcag
     3181 aaacagtcat gcacttctca tgtgttggct acaggtacca cacaaaagct aaaacctcca
     3241 ccttcttcac ttccaccccc aacccccatc tccggtcaga gccccagttc acaaaagtcc
     3301 agcacggcca cttcttctcc ctctccccag agttcttcca gccagtgcag tgtgccctcc
     3361 ctgagcagcc ttaccactaa cagccagctg aaggcactct ttgatgggct ttctcatatc
     3421 tataccactc agggacagtc tcgcaaaaag ggacacccga gttatgcacc acccaaacgt
     3481 atgcgtcgta aaactgaatt atcttccacg gcaaaatcta aagcccactt ctttggcaaa
     3541 agagatatta gaagtcggtt tatttctcac tcctcctcct ctagctgggg gatggctaga
     3601 ggaagtattt ttaaagcaat tgctcacttc aagcgaacaa ctttccttaa aaagcacagg
     3661 atgctaggca gattaaaata taaagtgacc cctcagatgg ggaccccctc accagggaag
     3721 gggagcttga cagacggaag gattaaacct gatcaggatg atgatactga aataaaaata
     3781 aacatcaaac aagaaagtgc agatgtaaat gtgattggaa acaaggatgt cgttactgaa
     3841 gaggatttgg atgtttttaa gcaggcccag gaactttctt gggagaaaat agagtgtgag
     3901 agtggggtgg aagactgtgg ccggtaccct tctgtgattg aatttggtaa atatgaaatc
     3961 caaacctggt actcctcgcc ttacccacag gaatatgcaa gattaccaaa gctttacctg
     4021 tgtgaattct gtcttaaata tatgaaaagt aaaaatattt tgctaagaca ctccaagaag
     4081 tgtggatggt ttcatcctcc agcaaatgaa atttaccgaa ggaaagacct ttcagtattt
     4141 gaggttgatg ggaatatgag caaaatttat tgccaaaacc tttgcttgtt agccaagctc
     4201 ttcctggacc acaaaacgtt gtattatgat gtcgagccat tcctttttta tgtccttaca
     4261 aaaaatgatg aaaagggctg tcatctggtt ggatacttct ctaaggaaaa gctttgccag
     4321 cagaagtata atgtctcctg cataatgatc atgccccagc accaaaggca aggatttgga
     4381 cggtttctca ttgatttcag ctatttgctt tctagaagag aaggccaagc agggtctcct
     4441 gaaaagcctc tctccgatct gggccgtctc tcctacctgg catattggaa gagcgtcatc
     4501 ttggagtatc tctaccacca ccatgagagg cacatcagca tcaaggcaat tagcagagcg
     4561 acgggcatgt gcccacatga cattgccacc actctgcagc acctccacat gatcgacaag
     4621 agagatggca gatttgtcat cattagacgg gaaaagttga tattgagcca catggaaaag
     4681 ctgaaaacct gttccagagc caatgaactt gatccagaca gtctgaggtg gaccccaatt
     4741 ttaatttcta atgctgcagt gtctgaagaa gagcgagaag ctgagaaaga ggctgagcgg
     4801 ctaatggaac aagctagctg ctgggagaag gaggaacaag aaatcctgtc aactagagct
     4861 aacagtaggc aatcacctgc aaaagtacaa tcgaaaaata aatatttgca ttccccggag
     4921 agccggccag tcacagggga gcgagggcag ctgctggagc tgtctaaaga gagcagtgaa
     4981 gaagaagagg aggaggagga cgaggaggag gaagaagagg aggaagaaga ggaagaggat
     5041 gaagaggagg aagaagagga agaagaagaa gaagaagaag aaaatattca aagctctccc
     5101 ccaagattga cgaaaccaca gtcagttgcc ataaagagaa agaggccttt tgtactaaag
     5161 aagaaaaggg gtcgtaaacg caggaggatc aacagcagtg taacaacaga gaccatttca
     5221 gagacgacag aagtactgaa tgagcccttt gacaactcag atgaagagag gccaatgcca
     5281 cagctggagc ctacctgtga gattgaagtg gaggaagatg gcaggaagcc agtcctgaga
     5341 aaagcattcc agcatcagcc tgggaagaaa agacaaacag aggaagagga aggaaaagac
     5401 aatcattgct tcaagaatgc tgacccttgt agaaacaata tgaatgatga ttcaagtaac
     5461 ttgaaagaag gcagtaaaga caatcccgaa cctctaaagt gcaaacaagt gtggccaaaa
     5521 ggaacaaagc gcggtctatc taagtggagg caaaacaaag agaggaagac cggatttaaa
     5581 ctgaatttgt acaccccgcc agaaacaccc atggagcctg acgagcaggt aacagtggaa
     5641 gaacagaagg agacttcaga aggaaaaacc agccccagtc ccatcaggat tgaggaggag
     5701 gtcaaggaaa ctggggaagc cctgttgcct caagaggaaa acagaaggga agaaacatgt
     5761 gcccctgtaa gtccaaacac atcaccaggt gaaaaaccag aagatgatct catcaaacct
     5821 gaggaagagg aagaggagga ggaggaggaa gaggaagaag aggaagaaga ggaaggggaa
     5881 gaagaagaag gaggaggaaa tgtagaaaaa gatccagatg gtgctaaaag ccaagaaaaa
     5941 gaggaaccag aaatctccac ggaaaaagaa gactctgcac gtttggatga tcacgaagag
     6001 gaggaggaag aggatgaaga gccatcccac aacgaggacc atgatgccga tgacgaggat
     6061 gacagccaca tggagtctgc cgaagtggag aaggaagagc tgcccagaga aagcttcaaa
     6121 gaagtactgg aaaaccagga gactttttta gaccttaatg tgcagcctgg tcactcgaac
     6181 ccagaggtct taatggactg tggcgtcgac ctgacagctt cttgtaacag tgagcccaag
     6241 gagcttgctg gggaccctga agctgtaccc gaatctgacg aggagccacc cccaggagaa
     6301 caggcacaga agcaggacca aaagaacagc aaggaagtcg atacagagtt caaagaggga
     6361 aacccagcaa ccatggaaat cgactctgag actgtccagg ccgttcagtc tttgacccag
     6421 gagagcagcg aacaggacga cacctttcag gattgtgccg agactcaaga ggcctgtaga
     6481 agcctacaga actacacccg tgcagaccaa agtccacaga ttgccaccac gctcgacgat
     6541 tgccaacagt cggaccacag tagcccagtt tcatccgtcc actcccatcc tggccagtcc
     6601 gtacgttctg tcaacagccc aagtgtccct gctctggaaa acagctacgc ccaaatcagc
     6661 ccagatcaaa gtgccatctc agtgccatct ctgcagaaca tggaaaccag tcccatgatg
     6721 gatgtcccat cagtttcaga tcattcacag caagtcgtag acagtggatt tagtgacctg
     6781 ggcagtatcg agagcacaac tgagaactac gaaaacccaa gcagctacga ttctactatg
     6841 ggaggcagca tctgtggaaa cggctcttca cagaacagct gctcctatag caacctcacc
     6901 tccagcagtc tgacacagag cagctgtgct gtcacccagc agatgtccaa catcagcggg
     6961 agctgcagca tgctgcagca aaccagcatc agctcccctc cgacctgcag cgtcaagtct
     7021 cctcaaggct gtgtggtgga gaggcctccg agcagcagcc agcagctggc tcagtgcagc
     7081 atggctgcta acttcacccc acccatgcag ctggctgaaa tccccgagac gagcaacgcc
     7141 aacattggct tatacgagcg aatgggtcag agtgattttg gggctgggca ttacccgcag
     7201 ccgtcagcca ccttcagcct tgccaaactg cagcagttaa ctaatacact tattgatcat
     7261 tcattgcctt acagccattc cgctgctgtg acttcctatg caaacagtgc ctctttgtcc
     7321 acaccattaa gtaacacagg gcttgttcaa ctttctcagt ctccacactc cgtccctggg
     7381 ggaccccaag cacaagctac catgacccca ccccccaacc tgactcctcc tccaatgaat
     7441 ctgccgccgc ctcttttgca acggaacatg gctgcatcaa atattggcat ctctcacagc
     7501 caaagactgc aaacccagat tgccagcaag ggccacatct ccatgagaac caagtcagcg
     7561 tctctgtcac cagccgctgc cacccatcag tcacaaatct atgggcgctc ccagactgta
     7621 gccatgcagg gtcctgcacg gactttaacg atgcaaagag gcatgaacat gagtgtgaac
     7681 ctgatgccag cgccagccta caatgtcaac tctgtgaaca tgaacatgaa cactctcaac
     7741 gccatgaatg ggtacagcat gtcccagcca atgatgaaca gtggctacca cagcaatcat
     7801 ggctatatga atcaaacgcc ccaataccct atgcagatgc agatgggcat gatgggcacc
     7861 cagccatatg cccagcagcc aatgcagacc ccaccccacg gtaacatgat gtacacggcc
     7921 cccggacatc acggctacat gaacacaggc atgtccaaac agtctctcaa tggctcctac
     7981 atgagaaggt agggtacccg ggcatgcctc gagctagcag atcttaatta attaaggatc
     8041 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     8101 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     8161 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     8221 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     8281 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     8341 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     8401 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     8461 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     8521 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8581 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8641 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     8701 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8761 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8821 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8881 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8941 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9001 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     9061 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9181 gaatcaggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9241 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9301 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9361 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9421 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9481 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9601 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9661 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     9721 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9781 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9841 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9901 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     9961 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10021 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10141 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10321 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    10801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    10861 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    10921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    10981 gggttccgcg cacatttccc cgaaaagtgc cacctgg
//
