LOCUS V024934 6485 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024934 VERSION V024934 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 4..383 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 386..661 /label="chicken β-actin promoter" intron 662..1674 /note="chimera between introns from chicken β-actin and rabbit β-globin" /label="chimeric intron" regulatory 1681..1690 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 1690..1755 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" CDS 1762..2694 /label="OR5L1(NM_001004738)" /note="OR5L1(NM_001004738)" /gene="OR5L1" CDS 2701..2721 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /transl_table=1 /translation="ENLYFQS" /label="TEV site" CDS 2722..3435 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" /label="EGFP" CDS 3436..3459 /codon_start=1 /product="peptide that binds Strep-Tactin®, an engineered form of streptavidin" /transl_table=1 /translation="WSHPQFEK" /label="Strep-Tag II" CDS 3478..3501 /codon_start=1 /product="peptide that binds Strep-Tactin®, an engineered form of streptavidin" /transl_table=1 /translation="WSHPQFEK" /label="Strep-Tag II" polyA_signal 3573..3628 /note="rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)" /label="β-globin poly(A) signal" primer_bind complement(3988..4004) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4012..4028 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4036..4066) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4081..4102 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 4161..4356 /note="SV40 early promoter" /label="SV40 promoter" polyA_signal 4362..4496 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4735..5323) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5494..6354) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6355..6459) /gene="bla" /label="AmpR promoter" ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc 1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag 1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc 1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc 1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg 1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 1621 ggcggctcta gtgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 1681 gccaccatgg actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag 1741 gatgacgatg acaagctcga gatgggcaag gaaaactgca ccactgtggc tgagttcatt 1801 ctccttggac tatcagatgt ccctgagttg agagtctgcc tcttcctgct gttccttctc 1861 atctatggag tcacgttgtt agccaacctg ggcatgattg cactgattca ggtcagctct 1921 cggctccaca cccccatgta ctttttcctc agccacttgt cctctgtaga tttctgctac 1981 tcctcaataa ttgtgccaaa aatgttggct aatatcttta acaaggacaa agccatctcc 2041 ttcctagggt gcatggtgca attctacttg ttttgcactt gtgtggtcac tgaggtcttc 2101 ctgctggccg tgatggccta tgaccgcttt gtggccatct gtaacccttt gctatacaca 2161 gtcaccatgt cttggaaggt gcgtgtggag ctggcttctt gctgctactt ctgtgggacg 2221 gtgtgttctc tgattcattt gtgcttagct cttaggatcc ccttctatag atctaatgtg 2281 attaaccact ttttctgtga tctacctcct gtcttaagtc ttgcttgctc tgatatcact 2341 gtgaatgaga cactgctgtt cctggtggcc actttgaatg agagtgttac catcatgatc 2401 atcctcacct cctacctgct aattctcacc accatcctga agatgggctc tgcagagggc 2461 aggcacaaag ccttctccac ctgtgcttcc cacctcacag ctatcactgt cttccatgga 2521 acagtccttt ccatttattg caggcccagt tcaggcaata gtggagatgc tgacaaagtg 2581 gccaccgtgt tctacacagt cgtgattcct atgctgaact ctgtgatcta cagcctgaga 2641 aataaagatg tgaaagaagc tctcagaaaa gtgatgggct ccaaaattca ctccgaattc 2701 gaaaacctgt acttccaatc cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2761 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 2821 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 2881 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 2941 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3001 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3061 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3121 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3181 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3241 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3301 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 3361 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3421 gacgagctgt acaagtggag ccacccgcag ttcgagaagg gcggcggcag cagcgcctgg 3481 agccacccgc agttcgagaa gtgaagatct ttttccctct gccaaaaatt atggggacat 3541 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 3601 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 3661 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc 3721 catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc tgctgtccat 3781 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg 3841 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc 3901 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag atccctcgac 3961 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4141 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact 4201 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 4261 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 4321 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt 4381 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 4441 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct 4501 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 4561 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4621 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4921 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 5041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 5101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 5161 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 5221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 5281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 5701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 5821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 5881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 5941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 6001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 6061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 6121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 6181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 6241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 6301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6481 acctg //