LOCUS V042954 6337 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042954 VERSION V042954 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers regulatory 4..13 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 13..78 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" CDS 82..1470 /label="PLIN5(NM_001013706)" /note="PLIN5(NM_001013706)" /gene="PLIN5" CDS 1477..1542 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1694..1749 /note="rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)" /label="β-globin poly(A) signal" primer_bind complement(2110..2126) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 2134..2150 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(2158..2188) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2202..2223 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 2282..2477 /note="SV40 early promoter" /label="SV40 promoter" polyA_signal 2483..2617 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2855..3443) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3614..4474) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(4475..4579) /gene="bla" /label="AmpR promoter" enhancer 4610..4989 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 4991..5268 /label="chicken β-actin promoter" intron 5269..6286 /note="chimera between introns from chicken β-actin and rabbit β-globin" /label="chimeric intron" ORIGIN 1 ttcgccacca tggactacaa agaccatgac ggtgattata aagatcatga catcgactac 61 aaggatgacg atgacaagct tatgtctgaa gaagaggcgg ctcagatccc cagatccagt 121 gtgtgggagc aggaccagca gaacgtggtg cagcgtgtgg tggctctgcc cctggtcagg 181 gccacgtgca ccgcggtctg cgatgtttac agtgcagcca aggacaggca cccgctgctg 241 ggctccgcct gccgcctggc tgagaactgc gtgtgcggcc tgaccacccg tgccctggac 301 cacgcccagc cgctgctcga gcacctgcag ccccagctgg ccactatgaa cagcctcgcc 361 tgcaggggcc tggacaagct ggaagagaag cttccctttc tccagcaacc ttcggagacg 421 gtggtgacct cagccaagga cgtggtggcc agcagtgtca cgggtgtggt ggacctggcc 481 cggaggggcc ggcgctggag cgtggagctg aagcgctccg tgagccatgc tgtggatgtt 541 gtactggaaa aatcagagga gctggtggat cacttcctgc ccatgacgga ggaagagctc 601 gcggcactgg cggctgaggc tgaaggccct gaagtgggtt cggtggagga tcagaggaga 661 cagcagggct actttgtgcg cctcggctcc ctgtcagcac ggatccgcca cctggcctac 721 gagcactctg tggggaaact gaggcagagc aaacaccgtg cccaggacac cctggcccag 781 ctgcaggaga cgctggagct gatagaccac atgcagtgtg gggtgacccc caccgccccg 841 gcctgccctg ggaaggtgca cgagctgtgg ggggaatggg gccagcgccc tccggagagc 901 cgccgccgga gccaggcaga gctggagacg ctggtgctgt cccgcagcct gacccaggag 961 ctgcagggca cggtagaggc tctggagtcc agcgtgcggg gcctgcccgc cggcgcccag 1021 gagaaggtgg ctgaggtgcg gcgcagtgtg gatgccctgc agaccgcctt cgctgatgcc 1081 cgctgcttca gggacgtgcc agcggccgcg ctggccgagg gccggggtcg cgtggcccac 1141 gcgcacgcct gcgtggacga gctgctggag ctggtggtgc aggccgtgcc gctgccctgg 1201 ctggtgggac ccttcgcgcc catccttgtg gagcgacccg agcccctgcc cgacctggcg 1261 gacctggtgg acgaggtcat cgggggccct gacccccgct gggcgcacct ggactggccg 1321 gcccagcaga gagcctggga ggcagagcac agggacggga gtgggaatgg ggatggggac 1381 aggatgggtg ttgccgggga catctgcgag caggaacccg agacccccag ctgcccggtc 1441 aagcacaccc tgatgcccga gctggacttc ggatccgact acaaagacca tgacggtgat 1501 tataaagatc atgacatcga ctacaaggat gacgatgaca agtaggaatt cactcctcag 1561 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac 1621 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 1681 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 1741 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 1801 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 1861 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 1921 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 1981 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 2041 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc 2101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 2161 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca 2221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc 2281 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 2341 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2401 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2461 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag 2521 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 2581 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa 2641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 2701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2761 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 2821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 2881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 2941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 3001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 3061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 3121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 3181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 3241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 3301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 3361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 3421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 3481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 3541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 3601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 3661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 3721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 3781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 3841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 3901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 3961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 4021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 4081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 4141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 4201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 4261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 4321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 4381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 4441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 4501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 4561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta 4621 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 4681 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 4741 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 4801 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 4861 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 4921 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 4981 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 5041 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 5101 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 5161 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 5221 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg 5281 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga 5341 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat 5401 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg 5461 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc 5521 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc 5581 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg 5641 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg 5701 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga 5761 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc 5821 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg 5881 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg 5941 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc 6001 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg 6061 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc 6121 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg 6181 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc 6241 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt 6301 gctggttatt gtgctgtctc atcattttgg caaagaa //