LOCUS       V042954                 6337 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042954
VERSION     V042954
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     regulatory      4..13
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             13..78
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             82..1470
                     /label="PLIN5(NM_001013706)"
                     /note="PLIN5(NM_001013706)"
                     /gene="PLIN5"
     CDS             1477..1542
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    1694..1749
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(2110..2126)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2134..2150
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2158..2188)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2202..2223
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        2282..2477
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    2483..2617
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2855..3443)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3614..4474)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4475..4579)
                     /gene="bla"
                     /label="AmpR promoter"
     enhancer        4610..4989
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        4991..5268
                     /label="chicken β-actin promoter"
     intron          5269..6286
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
ORIGIN
        1 ttcgccacca tggactacaa agaccatgac ggtgattata aagatcatga catcgactac
       61 aaggatgacg atgacaagct tatgtctgaa gaagaggcgg ctcagatccc cagatccagt
      121 gtgtgggagc aggaccagca gaacgtggtg cagcgtgtgg tggctctgcc cctggtcagg
      181 gccacgtgca ccgcggtctg cgatgtttac agtgcagcca aggacaggca cccgctgctg
      241 ggctccgcct gccgcctggc tgagaactgc gtgtgcggcc tgaccacccg tgccctggac
      301 cacgcccagc cgctgctcga gcacctgcag ccccagctgg ccactatgaa cagcctcgcc
      361 tgcaggggcc tggacaagct ggaagagaag cttccctttc tccagcaacc ttcggagacg
      421 gtggtgacct cagccaagga cgtggtggcc agcagtgtca cgggtgtggt ggacctggcc
      481 cggaggggcc ggcgctggag cgtggagctg aagcgctccg tgagccatgc tgtggatgtt
      541 gtactggaaa aatcagagga gctggtggat cacttcctgc ccatgacgga ggaagagctc
      601 gcggcactgg cggctgaggc tgaaggccct gaagtgggtt cggtggagga tcagaggaga
      661 cagcagggct actttgtgcg cctcggctcc ctgtcagcac ggatccgcca cctggcctac
      721 gagcactctg tggggaaact gaggcagagc aaacaccgtg cccaggacac cctggcccag
      781 ctgcaggaga cgctggagct gatagaccac atgcagtgtg gggtgacccc caccgccccg
      841 gcctgccctg ggaaggtgca cgagctgtgg ggggaatggg gccagcgccc tccggagagc
      901 cgccgccgga gccaggcaga gctggagacg ctggtgctgt cccgcagcct gacccaggag
      961 ctgcagggca cggtagaggc tctggagtcc agcgtgcggg gcctgcccgc cggcgcccag
     1021 gagaaggtgg ctgaggtgcg gcgcagtgtg gatgccctgc agaccgcctt cgctgatgcc
     1081 cgctgcttca gggacgtgcc agcggccgcg ctggccgagg gccggggtcg cgtggcccac
     1141 gcgcacgcct gcgtggacga gctgctggag ctggtggtgc aggccgtgcc gctgccctgg
     1201 ctggtgggac ccttcgcgcc catccttgtg gagcgacccg agcccctgcc cgacctggcg
     1261 gacctggtgg acgaggtcat cgggggccct gacccccgct gggcgcacct ggactggccg
     1321 gcccagcaga gagcctggga ggcagagcac agggacggga gtgggaatgg ggatggggac
     1381 aggatgggtg ttgccgggga catctgcgag caggaacccg agacccccag ctgcccggtc
     1441 aagcacaccc tgatgcccga gctggacttc ggatccgact acaaagacca tgacggtgat
     1501 tataaagatc atgacatcga ctacaaggat gacgatgaca agtaggaatt cactcctcag
     1561 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     1621 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     1681 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     1741 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     1801 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     1861 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     1921 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     1981 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     2041 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     2101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2161 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
     2221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     2281 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2341 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2401 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2461 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     2521 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2581 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     2641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2761 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     4621 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4681 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     4741 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     4801 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     4861 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4921 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4981 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     5041 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     5101 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     5161 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     5221 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg
     5281 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     5341 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     5401 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     5461 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     5521 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     5581 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     5641 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     5701 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     5761 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     5821 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     5881 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     5941 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     6001 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     6061 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     6121 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     6181 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     6241 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     6301 gctggttatt gtgctgtctc atcattttgg caaagaa
//
