LOCUS       V022495                 5989 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022495
VERSION     V022495
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1741..2994
                     /label="TMPRSS11D(NM_004262)"
                     /note="TMPRSS11D(NM_004262)"
                     /gene="TMPRSS11D"
     polyA_signal    3077..3132
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(3493..3509)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3517..3533
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3541..3571)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3585..3606
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        3665..3860
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    3866..4000
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4238..4826)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4997..5857)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5858..5962)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc
     1741 atgtataggc cagcacgtgt aacttcgact tcaagatttc tgaatccata tgtagtatgt
     1801 ttcattgtcg tcgcaggggt agtgatcctg gcagtcacca tagctctact tgtttacttt
     1861 ttagcttttg atcaaaaatc ttacttttat aggagcagtt ttcaactcct aaatgttgaa
     1921 tataatagtc agttaaattc accagctaca caggaataca ggactttgag tggaagaatt
     1981 gaatctctga ttactaaaac attcaaagaa tcaaatttaa gaaatcagtt catcagagct
     2041 catgttgcca aactgaggca agatggtagt ggtgtgagag cggatgttgt catgaaattt
     2101 caattcacta gaaataacaa tggagcatca atgaaaagca gaattgagtc tgttttacga
     2161 caaatgctga ataactctgg aaacctggaa ataaaccctt caactgagat aacatcactt
     2221 actgaccagg ctgcagcaaa ttggcttatt aatgaatgtg gggccggtcc agacctaata
     2281 acattgtctg agcagagaat ccttggaggc actgaggctg aggagggaag ctggccgtgg
     2341 caagtcagtc tgcggctcaa taatgcccac cactgtggag gcagcctgat caataacatg
     2401 tggatcctga cagcagctca ctgcttcaga agcaactcta atcctcgtga ctggattgcc
     2461 acgtctggta tttccacaac atttcctaaa ctaagaatga gagtaagaaa tattttaatt
     2521 cataacaatt ataaatctgc aactcatgaa aatgacattg cacttgtgag acttgagaac
     2581 agtgtcacct ttaccaaaga tatccatagt gtgtgtctcc cagctgctac ccagaatatt
     2641 ccacctggct ctactgctta tgtaacagga tggggcgctc aagaatatgc tggccacaca
     2701 gttccagagc taaggcaagg acaggtcaga ataataagta atgatgtatg taatgcacca
     2761 catagttata atggagccat cttgtctgga atgctgtgtg ctggagtacc tcaaggtgga
     2821 gtggacgcat gtcagggtga ctctggtggc ccactagtac aagaagactc acggcggctt
     2881 tggtttattg tggggatagt aagctgggga gatcagtgtg gcctgccgga taagccagga
     2941 gtgtatactc gagtgacagc ctaccttgac tggattaggc aacaaactgg gatctagctc
     3001 gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     3061 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     3121 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     3181 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     3241 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     3301 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     3361 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     3421 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     3481 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3541 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     3601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     3661 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3721 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3781 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3841 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     3901 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3961 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     4021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4141 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgg
//
