LOCUS       Exported               10549 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            hygromycin- and kanamycin-resistance and GFP genes.
ACCESSION   AF234298
VERSION     .
KEYWORDS    pCAMBIA1302
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Cambia
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The GenBank record was corrected by inserting a G at position 3443.
FEATURES             Location/Qualifiers
     source          1..10549
                     /lab_host="Plant Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..727
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="mgfp5"
                     /note="suitable for expression in plants due to removal of
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             734..751
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis affinity tag
                     /note="6xHis"
                     /translation="HHHHHH"
     terminator      783..1035
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1057..1081
                     /label=RB T-DNA repeat
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2382..3011
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=stability protein from Pseudomonas plasmid pVS1
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3440..4513
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /label=replication protein from Pseudomonas plasmid
                     pVS1
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4579..4773
                     /label=pVS1 oriV
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    5117..5257
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5443..6031)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6118..6912)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=aphA-3
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7337..7361
                     /label=LB T-DNA repeat
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7439..7613
                     /label=CaMV poly(A) signal
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7654..8679)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=hygromycin B phosphotransferase
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8746..9422)
                     /note="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     promoter        9649..9679
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9687..9703
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9711..9727
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             9723..9955
                     /codon_start=1
                     /gene="lacZ (truncated)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="lacZ-alpha"
                     /translation="MT*LRIRARYPGIL*SRPAGMQAWHWPSFYNVVTGKTLALPNLIA
                     LQHIPLSPAGVIAKRPAPIALPNSCAA*MANA"
     misc_feature    9736..9792
                     /label=MCS
                     /note="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9796..9812)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10189..10534
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt
       61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga
      241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag
      301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg
      361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat
      421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa
      481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt
      541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc
      781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
      841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
      901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
      961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1081 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     1141 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac
     1201 tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
     1261 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
     1321 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
     1381 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
     1441 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
     1501 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
     1561 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
     1621 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
     1681 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
     1741 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
     1801 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
     1861 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
     1921 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
     1981 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
     2041 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
     2101 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
     2161 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
     2221 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
     2281 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     2341 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     2401 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     2461 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     2521 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     2581 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     2641 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     2701 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     2761 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     2821 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     2881 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     2941 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     3001 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     3061 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     3121 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     3181 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     3241 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     3301 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     3361 gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc actggaaccc
     3421 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     3481 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     3541 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     3601 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     3661 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     3721 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     3781 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     3841 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     3901 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     3961 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     4021 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     4081 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     4141 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     4201 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     4261 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     4321 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     4381 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     4441 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     4501 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     4561 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     4621 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     4681 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     4741 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     4801 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     4861 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     4921 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     4981 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5041 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5101 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     5161 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     5221 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     5281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5881 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta
     6121 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
     6181 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
     6241 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
     6301 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
     6361 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
     6421 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
     6481 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
     6541 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
     6601 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
     6661 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
     6721 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
     6781 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
     6841 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
     6901 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
     6961 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
     7021 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
     7081 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
     7141 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
     7201 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
     7261 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     7321 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     7381 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga
     7441 tctggatttt agtactggat tttggtttta ggaattagaa attttattga tagaagtatt
     7501 ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta
     7561 taggaaccct aattccctta tctgggaact actcacacat tattatggag aaactcgagc
     7621 ttgtcgatcg acagatcccg gtcggcatct actctatttc tttgccctcg gacgagtgct
     7681 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     7741 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     7801 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     7861 ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg
     7921 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     7981 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     8041 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     8101 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     8161 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     8221 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     8281 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg
     8341 ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgaacg
     8401 gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat
     8461 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     8521 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     8581 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     8641 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccggatct
     8701 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     8761 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     8821 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     8881 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     8941 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     9001 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     9061 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     9121 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat
     9181 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     9241 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     9301 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     9361 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     9421 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     9481 cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg
     9541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9721 ctatgacatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
     9781 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     9961 cttgagcttg gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat
    10021 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct
    10081 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt
    10141 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
    10201 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
    10261 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
    10321 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
    10381 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    10441 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
    10501 tatataagga agttcatttc atttggagag aacacggggg actcttgac
//
