LOCUS 40924_9216 4796 bp DNA circular SYN 17-DEC-2018 DEFINITION Luciferase reporter vector pCBR-Basic, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4796) AUTHORS Almond B, Chauvin F. TITLE Chroma-Luc? Series Reporter Vectors Technical Manual #TM061 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4796) AUTHORS Almond B, Chauvin F. TITLE Direct Submission JOURNAL Submitted (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA REFERENCE 3 (bases 1 to 4796) TITLE Direct Submission REFERENCE 4 (bases 1 to 4796) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (20-MAR-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4796 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..58 /label=multiple cloning region /note="multiple cloning region" CDS 88..1713 /codon_start=1 /label=CBRluc /note="red-emitting variant of click beetle luciferase" /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP RYGTQLIPGVTVLVYLPFFHAFGFHITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT ESTSAIIQTLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN PCIRDVAVVGIPDLEAGELPSAFVVKQPGTEITAKEVYDYLAERVSHTKYLRGGVRFVD SIPRNVTGKITRKELLKQLLVKAGG" polyA_signal complement(1759..1880) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 2296 /label=ColE1-derived /note="ColE1-derived" rep_origin complement(2299..2887) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3061..3918) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3919..4023) /label=AmpR promoter rep_origin 4050..4505 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" polyA_signal 4636..4684 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 4698..4789 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg 61 cattccggta ctgttggtaa agccaccatg gtaaagcgtg agaaaaatgt catctatggc 121 cctgagcctc tccatccttt ggaggatttg actgccggcg aaatgctgtt tcgtgctctc 181 cgcaagcact ctcatttgcc tcaagccttg gtcgatgtgg tcggcgatga atctttgagc 241 tacaaggagt tttttgaggc aaccgtcttg ctggctcagt ccctccacaa ttgtggctac 301 aagatgaacg acgtcgttag tatctgtgct gaaaacaata cccgtttctt cattccagtc 361 atcgccgcat ggtatatcgg tatgatcgtg gctccagtca acgagagcta cattcccgac 421 gaactgtgta aagtcatggg tatctctaag ccacagattg tcttcaccac taagaatatt 481 ctgaacaaag tcctggaagt ccaaagccgc accaacttta ttaagcgtat catcatcttg 541 gacactgtgg agaatattca cggttgcgaa tctttgccta atttcatctc tcgctattca 601 gacggcaaca tcgcaaactt taaaccactc cacttcgacc ctgtggaaca agttgcagcc 661 attctgtgta gcagcggtac tactggactc ccaaagggag tcatgcagac ccatcaaaac 721 atttgcgtgc gtctgatcca tgctctcgat ccacgctacg gcactcagct gattcctggt 781 gtcaccgtct tggtctactt gcctttcttc catgctttcg gctttcatat tactttgggt 841 tactttatgg tcggtctccg cgtgattatg ttccgccgtt ttgatcagga ggctttcttg 901 aaagccatcc aagattatga agtccgcagt gtcatcaacg tgcctagcgt gatcctgttt 961 ttgtctaaga gcccactcgt ggacaagtac gacttgtctt cactgcgtga attgtgttgc 1021 ggtgccgctc cactggctaa ggaggtcgct gaagtggccg ccaaacgctt gaatcttcca 1081 gggattcgtt gtggcttcgg cctcaccgaa tctaccagtg cgattatcca gactctcggg 1141 gatgagttta agagcggctc tttgggccgt gtcactccac tcatggctgc taagatcgct 1201 gatcgcgaaa ctggtaaggc tttgggcccg aaccaagtgg gcgagctgtg tatcaaaggc 1261 cctatggtga gcaagggtta tgtcaataac gttgaagcta ccaaggaggc catcgacgac 1321 gacggctggt tgcattctgg tgattttgga tattacgacg aagatgagca tttttacgtc 1381 gtggatcgtt acaaggagct gatcaaatac aagggtagcc aggttgctcc agctgagttg 1441 gaggagattc tgttgaaaaa tccatgcatt cgcgatgtcg ctgtggtcgg cattcctgat 1501 ctggaggccg gcgaactgcc ttctgctttc gttgtcaagc agcctggtac agaaattacc 1561 gccaaagaag tgtatgatta cctggctgaa cgtgtgagcc atactaagta cttgcgtggc 1621 ggcgtgcgtt ttgttgactc catccctcgt aacgtaacag gcaaaattac ccgcaaggag 1681 ctgttgaaac aattgttggt gaaggccggc ggttagtaat ctagagtcgg ggcggccggc 1741 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc 1801 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta 1861 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg 1921 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata 1981 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg 2041 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga 2101 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 2161 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 2221 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 2281 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 2341 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 2401 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 2461 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 2521 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 2581 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 2641 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 2701 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 2761 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 2821 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 2881 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 2941 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 3001 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 3061 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 3121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 3181 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 3241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 3301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 3361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 3421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 3481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 3541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 3601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 3661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 3721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 3781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 3841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 3901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 3961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 4021 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc 4081 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 4141 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 4201 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 4261 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 4321 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 4381 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 4441 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 4501 aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 4561 ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt 4621 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg 4681 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta 4741 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata //