LOCUS       V020397                12310 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020397
VERSION     V020397
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2294..6457
                     /label="ROCK2(NM_004850)"
                     /note="ROCK2(NM_004850)"
                     /gene="ROCK2"
     CDS             6458..6481
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     promoter        6531..6742
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6755..7023
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7058..7750
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             7751..7804
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7805..8404
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8405..8993
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9067..9300
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9372..9493
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9512..9647
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9685..9701)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9709..9725
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9733..9763)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9778..9799
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10087..10675)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10846..11706)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11707..11811)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12285..12301
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatgagcc ggcccccgcc gacggggaaa atgcccggcg cccccgagac
     2341 cgcgccgggg gacggggcag gcgcgagccg ccagaggaag ctggaggcgc tgatccgaga
     2401 ccctcgctcc cccatcaacg tggagagctt gctggatggc ttaaattcct tggtccttga
     2461 tttagatttt cctgctttga ggaaaaacaa gaacatagat aatttcttaa atagatatga
     2521 gaaaattgtg aaaaaaatca gaggtctaca gatgaaggca gaagactatg atgttgtaaa
     2581 agttattgga agaggtgctt ttggtgaagt gcagttggtt cgtcacaagg catcgcagaa
     2641 ggtttatgct atgaagcttc ttagtaagtt tgaaatgata aaaagatcag attctgcctt
     2701 tttttgggaa gaaagagata ttatggcctt tgccaatagc ccctgggtgg ttcagctttt
     2761 ttatgccttt caagatgata ggtatctgta catggtaatg gagtacatgc ctggtggaga
     2821 ccttgtaaac cttatgagta attatgatgt gcctgaaaaa tgggccaaat tttacactgc
     2881 tgaagttgtt cttgctctgg atgcaataca ctccatgggt ttaatacaca gagatgtgaa
     2941 gcctgacaac atgctcttgg ataaacatgg acatctaaaa ttagcagatt ttggcacgtg
     3001 tatgaagatg gatgaaacag gcatggtaca ttgtgataca gcagttggaa caccggatta
     3061 tatatcacct gaggttctga aatcacaagg gggtgatggt ttctatgggc gagaatgtga
     3121 ttggtggtct gtaggtgttt tcctttatga gatgctagtg ggggatactc cattttatgc
     3181 ggattcactt gtaggaacat atagcaaaat tatggatcat aagaattcac tgtgtttccc
     3241 tgaagatgca gaaatttcca aacatgcaaa gaatctcatc tgtgctttct taacagatag
     3301 ggaggtacga cttgggagaa atggggtgga agaaatcaga cagcatcctt tctttaagaa
     3361 tgatcagtgg cattgggata acataagaga aacggcagct cctgtagtac ctgaactcag
     3421 cagtgacata gacagcagca atttcgatga cattgaagat gacaaaggag atgtagaaac
     3481 cttcccaatt cctaaagctt ttgttggaaa tcagctgcct ttcatcggat ttacctacta
     3541 tagagaaaat ttattattaa gtgactctcc atcttgtaga gaaactgatt ccatacaatc
     3601 aaggaaaaat gaagaaagtc aagagattca gaaaaaactg tatacattag aagaacatct
     3661 tagcaatgag atgcaagcca aagaggaact ggaacagaag tgcaaatctg ttaatactcg
     3721 cctagaaaaa acagcaaagg agctagaaga ggagattacc ttacggaaaa gtgtggaatc
     3781 agcattaaga cagttagaaa gagaaaaggc gcttcttcag cacaaaaatg cagaatatca
     3841 gaggaaagct gatcatgaag cagacaaaaa acgaaatttg gaaaatgatg ttaacagctt
     3901 aaaagatcaa cttgaagatt tgaaaaaaag aaatcaaaac tctcaaatat ccactgagaa
     3961 agtgaatcaa ctccagagac aactggatga aaccaatgct ttactgcgaa cagagtctga
     4021 tactgcagcc cggttaagga aaacccaggc agaaagttca aaacagattc agcagctgga
     4081 atctaacaat agagatctac aagataaaaa ctgcctgctg gagactgcca agttaaaact
     4141 tgaaaaggaa tttatcaatc ttcagtcagc tctagaatct gaaaggaggg atcgaaccca
     4201 tggatcagag ataattaatg atttacaagg tagaatatgt ggcctagaag aagatttaaa
     4261 gaacggcaaa atcttactag cgaaagtaga actggagaag agacaacttc aggagagatt
     4321 tactgatttg gaaaaggaaa aaagcaacat ggaaatagat atgacatacc aactaaaagt
     4381 tatacagcag agcctagaac aagaagaagc tgaacataag gccacaaagg cacgactagc
     4441 agataaaaat aagatctatg agtccatcga agaagccaaa tcagaagcca tgaaagaaat
     4501 ggagaagaag ctcttggagg aaagaacttt aaaacagaaa gtggagaacc tattgctaga
     4561 agctgagaaa agatgttctc tattagactg tgacctcaaa cagtcacagc agaaaataaa
     4621 tgagctcctt aaacagaaag atgtgctaaa tgaggatgtt agaaacctga cattaaaaat
     4681 agagcaagaa actcagaagc gctgccttac acaaaatgac ctgaagatgc aaacacaaca
     4741 ggttaacaca ctaaaaatgt cagaaaagca gttaaagcaa gaaaataacc atctcatgga
     4801 aatgaaaatg aacttggaaa aacaaaatgc tgaacttcga aaagaacgtc aggatgcaga
     4861 tgggcaaatg aaagagctcc aggatcagct cgaagcagaa cagtatttct caacccttta
     4921 taaaacacaa gttagggagc ttaaagaaga atgtgaagaa aagaccaaac ttggtaaaga
     4981 attgcagcag aagaaacagg aattacagga tgaacgggac tctttggctg cccaactgga
     5041 gatcaccttg accaaagcag attctgagca actggctcgt tcaattgctg aagaacaata
     5101 ttctgatttg gaaaaagaga agatcatgaa agagctggag atcaaagaga tgatggctag
     5161 acacaaacag gaacttacgg aaaaagatgc tacaattgct tctcttgagg aaactaatag
     5221 gacactaact agtgatgttg ccaatcttgc aaatgagaaa gaagaattaa ataacaaatt
     5281 gaaagatgtt caagagcaac tgtcaagatt gaaagatgaa gaaataagcg cagcagctat
     5341 taaagcacag tttgagaagc agctattaac agaaagaaca ctcaaaactc aagctgtgaa
     5401 taagttggct gagatcatga atcgaaaaga acctgtcaag cgtggtaatg acacagatgt
     5461 gcggagaaaa gagaaggaga atagaaagct acatatggag cttaaatctg aacgtgagaa
     5521 attgacccag cagatgatca agtatcagaa agaactgaat gaaatgcagg cacaaatagc
     5581 tgaagagagc cagattcgaa ttgaactgca gatgacattg gacagtaaag acagtgacat
     5641 tgagcagctg cggtcacaac tccaagcctt gcatattggt ctggatagtt ccagtatagg
     5701 cagtggacca ggggatgctg aggcagatga tgggtttcca gaatcaagat tagaaggatg
     5761 gctttcattg cctgtacgaa acaacactaa gaaatttgga tgggttaaaa agtatgtgat
     5821 tgtaagcagt aagaagattc ttttctatga cagtgaacaa gataaagaac aatccaatcc
     5881 ttacatggtt ttagatatag acaagttatt tcatgtccga ccagttacac agacagatgt
     5941 gtatagagca gatgctaaag aaattccaag gatattccag attctgtatg ccaatgaagg
     6001 agaaagtaag aaggaacaag aatttccagt ggagccagtt ggagaaaaat ctaattatat
     6061 ttgccacaag ggacatgagt ttattcctac tctttatcat ttcccaacca actgtgaggc
     6121 ttgtatgaag cccctgtggc acatgtttaa gcctcctcct gctttggagt gccgccgttg
     6181 ccatattaag tgtcataaag atcatatgga caaaaaggag gagattatag caccttgcaa
     6241 agtatattat gatatttcaa cggcaaagaa tctgttatta ctagcaaatt ctacagaaga
     6301 gcagcagaag tgggttagtc ggttggtgaa aaagatacct aaaaagcccc cagctccaga
     6361 cccttttgcc cgatcatctc ctagaacttc aatgaagata cagcaaaacc agtctattag
     6421 acggccaagt cgacagcttg ccccaaacaa acctagcgat tacaaggacg acgatgacaa
     6481 gtaaggatcc gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     6541 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     6601 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     6661 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     6721 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     6781 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     6841 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     6901 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     6961 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     7021 atccaagctg tgaccggcgc ctacgctaga cgccaccatg agcgagctga tcaaggagaa
     7081 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     7141 cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg tggtcgaggg
     7201 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaagc
     7261 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt
     7321 cacatgggag agaatcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     7381 cagcttccag aacggctgca tcatctacaa cgtcaagatc aacggggtga acttcccatc
     7441 caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg agatgctgta
     7501 ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg tgggcggggg
     7561 ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta agaacctcaa
     7621 gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg ccgacaaaga
     7681 gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc ctagcaaact
     7741 ggggcacaga gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
     7801 ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     7861 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     7921 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     7981 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     8041 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8101 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8161 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8221 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8281 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     8341 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     8401 ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     8461 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     8521 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     8581 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     8641 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     8701 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     8761 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     8821 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8881 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8941 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac
     9001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9061 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg
     9121 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9181 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9241 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9301 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     9361 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9481 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc
     9541 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     9601 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag
     9661 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     9721 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     9781 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     9841 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9901 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    10441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    10501 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    10561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    10621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    10801 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    10861 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10921 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10981 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11041 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11101 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    11161 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    11221 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    11281 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    11341 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    11401 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    11461 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    11521 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    11581 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    11641 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    11701 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11761 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11821 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    11881 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    11941 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    12001 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    12061 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    12121 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    12181 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    12241 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    12301 tgccaagctg
//
