LOCUS       V033531                 9630 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033531
VERSION     V033531
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1494..1538
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1801..1916
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        1974..2277
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2278..2481
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2588..3700
                     /label="ELF3(NM_001114309)"
                     /note="ELF3(NM_001114309)"
                     /gene="ELF3"
     CDS             3725..3796
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     promoter        3850..4061
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4074..4342
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4370..5062
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Zoanthus green fluorescent protein"
                     /transl_table=1
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
                     /label="ZsGreen1"
     CDS             5072..5125
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5126..5725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5726..6313
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6387..6620
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6692..6813
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6832..6967
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7005..7021)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7029..7045
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7053..7083)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7098..7119
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7407..7995)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8166..9026)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9027..9131)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9605..9621
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     1921 gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca
     1981 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2041 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2101 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2161 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2221 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2281 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2341 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2401 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2461 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2521 cgctgttttg acctccatag aagacaccga ctctactaga ggatctattt ccggtgaatt
     2581 cgccaccatg gctgcaacct gtgagattag caacattttt agcaactact tcagtgcgat
     2641 gtacagctcg gaggactcca ccctggcctc tgttccccct gctgccacct ttggggccga
     2701 tgacttggta ctgaccctga gcaaccccca gatgtcattg gagggtacag agaaggccag
     2761 ctggttgggg gaacagcccc agttctggtc gaagacgcag gttctggact ggatcagcta
     2821 ccaagtggag aagaacaagt acgacgcaag cgccattgac ttctcacgat gtgacatgga
     2881 tggcgccacc ctctgcaatt gtgcccttga ggagctgcgt ctggtctttg ggcctctggg
     2941 ggaccaactc catgcccagc tgcgagacct cacttccagc tcttctgatg agctcagttg
     3001 gatcattgag ctgctggaga aggatggcat ggccttccag gaggccctag acccagggcc
     3061 ctttgaccag ggcagcccct ttgcccagga gctgctggac gacggtcagc aagccagccc
     3121 ctaccacccc ggcagctgtg gcgcaggagc cccctcccct ggcagctctg acgtctccac
     3181 cgcagggact ggtgcttctc ggagctccca ctcctcagac tccggtggaa gtgacgtgga
     3241 cctggatccc actgatggca agctcttccc cagcgatggt tttcgtgact gcaagaaggg
     3301 ggatcccaag cacgggaagc ggaaacgagg ccggccccga aagctgagca aagagtactg
     3361 ggactgtctc gagggcaaga agagcaagca cgcgcccaga ggcacccacc tgtgggagtt
     3421 catccgggac atcctcatcc acccggagct caacgagggc ctcatgaagt gggagaatcg
     3481 gcatgaaggc gtcttcaagt tcctgcgctc cgaggctgtg gcccaactat ggggccaaaa
     3541 gaaaaagaac agcaacatga cctacgagaa gctgagccgg gccatgaggt actactacaa
     3601 acgggagatc ctggaacggg tggatggccg gcgactcgtc tacaagtttg gcaaaaactc
     3661 aagcggctgg aaggaggaag aggttctcca gagtcggaac ggatcctcgg taccaagctt
     3721 aagtgactac aaggatgacg atgacaagga ttacaaagac gacgatgata aggactataa
     3781 ggatgatgac gacaaataaa gatccatcga tactagtaag gatctgcgat cgctccggtg
     3841 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     3901 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     3961 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     4021 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     4081 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     4141 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     4201 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     4261 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     4321 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tactctagca tggcccagtc
     4381 caagcacggc ctgaccaagg agatgaccat gaagtaccgc atggagggct gcgtggacgg
     4441 ccacaagttc gtgatcaccg gcgagggcat cggctacccc ttcaagggca agcaggccat
     4501 caacctgtgc gtggtggagg gcggcccctt gcccttcgcc gaggacatct tgtccgccgc
     4561 cttcatgtac ggcaaccgcg tgttcaccga gtacccccag gacatcgtcg actacttcaa
     4621 gaactcctgc cccgccggct acacctggga ccgctccttc ctgttcgagg acggcgccgt
     4681 gtgcatctgc aacgccgaca tcaccgtgag cgtggaggag aactgcatgt accacgagtc
     4741 caagttctac ggcgtgaact tccccgccga cggccccgtg atgaagaaga tgaccgacaa
     4801 ctgggagccc tcctgcgaga agatcatccc cgtgcccaag cagggcatct tgaagggcga
     4861 cgtgagcatg tacctgctgc tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt
     4921 gtacaaggcc aagtccgtgc cccgcaagat gcccgactgg cacttcatcc agcacaagct
     4981 gacccgcgag gaccgcagcg acgccaagaa ccagaagtgg cacctgaccg agcacgccat
     5041 cgcctccggc tccgccttgc ccgcggccgc tgagggcaga ggaagtcttc taacatgcgg
     5101 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac
     5161 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     5221 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     5281 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     5341 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     5401 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     5461 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     5521 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     5581 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     5641 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     5701 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
     5761 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5821 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     5881 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     5941 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6001 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6061 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6121 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6181 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6241 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     6301 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6361 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     6421 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6481 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6541 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6601 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     6661 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     6721 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     6841 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6901 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6961 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     7021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7201 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     7861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9241 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9301 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9361 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9421 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     9481 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9541 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9601 cgttgtaaaa cgacggccag tgccaagctg
//
