LOCUS       V044058                10650 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044058
VERSION     V044058
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1494..1538
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1801..1916
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        1974..2277
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2278..2481
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2588..4720
                     /label="Tbx2(NM_009324)"
                     /note="Tbx2(NM_009324)"
                     /gene="Tbx2"
     CDS             4745..4816
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     promoter        4870..5081
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5094..5362
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5390..6082
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Zoanthus green fluorescent protein"
                     /transl_table=1
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
                     /label="ZsGreen1"
     CDS             6092..6145
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6146..6745
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6746..7333
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7407..7640
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7712..7833
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7852..7987
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8025..8041)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8049..8065
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8073..8103)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8118..8139
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8427..9015)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9186..10046)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10047..10151)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10625..10641
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     1921 gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca
     1981 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2041 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2101 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2161 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2221 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2281 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2341 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2401 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2461 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2521 cgctgttttg acctccatag aagacaccga ctctactaga ggatctattt ccggtgaatt
     2581 cgccaccatg agagagccgg cgctggcagc cagcgccatg gcttaccacc cgttccacgc
     2641 gccacggccc gccgacttcc ccatgtccgc cttcctggcg gcagcgcagc cctccttctt
     2701 cccggcgcta gcgctgccgc ccggcgcgct aggcaagcct ctgccggacc cgggcctggc
     2761 gggggcggcg gccgcggcag cagcggcggc ggcggcggcc gaggcggggc tgcacgtctc
     2821 ggcactcggc ccgcacccgc ccgccgcgca tctgcgctcg ctcaagagcc tggaacccga
     2881 ggacgaggtg gaggacgacc ccaaggtgac gctggaggcc aaggagctgt gggaccagtt
     2941 ccacaagctg ggcaccgaga tggtcatcac caagtccggg aggcggatgt tccccccctt
     3001 caaggtgcga gtcagcggcc tggacaagaa agccaaatac atcctgctaa tggacatcgt
     3061 ggctgccgat gactgccgct ataagtttca caactcccgc tggatggtgg caggcaaagc
     3121 cgaccccgag atgcctaaac gcatgtacat ccacccggac agtccggcca caggggaaca
     3181 gtggatggcc aagcctgtgg ccttccacaa actgaagctg accaacaaca tttctgacaa
     3241 gcatggcttt accatcctga actccatgca caagtaccag ccgcgattcc acatcgtgcg
     3301 agccaatgac atcttgaagc tcccatacag caccttccgc acctatgtct tcccagagac
     3361 cgacttcatc gctgtcactg cctaccagaa tgacaagatt acacagctga agatcgacaa
     3421 caaccccttt gccaagggct tccgggacac tgggaacggc cgtcgggaga aaaggaagca
     3481 gctaacgctg cccactctcc gtttgtatga ggagcactgt aagccggagc gtgatggcgc
     3541 tgaatccgat gcctcgtcct gcgaccctcc gcctgcgcga gaaccaccgc cctcccccag
     3601 cgcagcgccc agccctctgc gcctacaccg ggcccgagcc gaggagaagc cgggcgcagc
     3661 cgacagcgat cctgagccgg agcggacggg ggaggagcgc tccgcagcgc ccctgggccg
     3721 cagcccttcg cgggacgcca gccccgctcg cttgaccgaa cccgaacgct cccgggagcg
     3781 gcgcagcccg gagaggtgca gcaaggaacc gacggagggc ggtggggacg gcccgtttag
     3841 cctgcggagc ctggagaagg agcgtcccga ggcccggagg aaggacgagg ggcgcaagga
     3901 cgtgggtgag ggcaaggagc ccagcctggc gccgctggtg gtgcagacag acagtgcgtc
     3961 ccccctgggt gcgggccatc tgcctggcct ggccttctcc agccacctgc atgggcagca
     4021 gttctttggg ccgctgggag ctggccagcc cctcttcctg cacccaggac aattcgccat
     4081 gggccccgga gccttctccg ccatgggcat gggtcatctg ctagcctcgg tggcaggcgg
     4141 cagtggcagt agcggaggcg ccggtcccgg aaccgcggcg gggctggacg caggcgggct
     4201 aggtcccgca gccagcgcag caagcaccgc ggctcccttc ccgttccacc tctcccagca
     4261 tatgctggca tctcagggaa tcccaatgcc cactttcgga ggcctctttc cctacccgta
     4321 cacttacatg gcagcagcgg ccgccgccgc ctccgcttta cccgccacca gcgctgcagc
     4381 tgccgccgcc gcagccgctg gttcattatc acggagccca tttctgggca gtgcccggcc
     4441 ccgtctgcgc ttcagtcctt accagatccc tgtcaccatc ccacctagca ctagcctcct
     4501 taccaccggg ctggcggcgg agggctccaa gggcggaaat agccgtgagc ccagtccgct
     4561 gcctgagctg gctctccgaa aagtgggggg cccatcccgc ggggccctat cgcccagcgg
     4621 ctcagccaaa gaggcggcca gtgaactgca gagcatccag agactggtga gtgggctaga
     4681 gagccagcga gccctctccc ccggccggga gtcgcccaag ggatcctcgg taccaagctt
     4741 aagtgactac aaggatgacg atgacaagga ttacaaagac gacgatgata aggactataa
     4801 ggatgatgac gacaaataaa gatccatcga tactagtaag gatctgcgat cgctccggtg
     4861 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     4921 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     4981 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     5041 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc
     5101 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc
     5161 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt
     5221 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca
     5281 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt
     5341 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tactctagca tggcccagtc
     5401 caagcacggc ctgaccaagg agatgaccat gaagtaccgc atggagggct gcgtggacgg
     5461 ccacaagttc gtgatcaccg gcgagggcat cggctacccc ttcaagggca agcaggccat
     5521 caacctgtgc gtggtggagg gcggcccctt gcccttcgcc gaggacatct tgtccgccgc
     5581 cttcatgtac ggcaaccgcg tgttcaccga gtacccccag gacatcgtcg actacttcaa
     5641 gaactcctgc cccgccggct acacctggga ccgctccttc ctgttcgagg acggcgccgt
     5701 gtgcatctgc aacgccgaca tcaccgtgag cgtggaggag aactgcatgt accacgagtc
     5761 caagttctac ggcgtgaact tccccgccga cggccccgtg atgaagaaga tgaccgacaa
     5821 ctgggagccc tcctgcgaga agatcatccc cgtgcccaag cagggcatct tgaagggcga
     5881 cgtgagcatg tacctgctgc tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt
     5941 gtacaaggcc aagtccgtgc cccgcaagat gcccgactgg cacttcatcc agcacaagct
     6001 gacccgcgag gaccgcagcg acgccaagaa ccagaagtgg cacctgaccg agcacgccat
     6061 cgcctccggc tccgccttgc ccgcggccgc tgagggcaga ggaagtcttc taacatgcgg
     6121 tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac
     6181 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     6241 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     6301 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     6361 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     6421 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     6481 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     6541 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6601 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6661 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     6721 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
     6781 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6841 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6901 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6961 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7021 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7081 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7141 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7201 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7261 ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga tctccctttg
     7321 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7381 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     7441 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7621 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7681 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7741 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7801 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7921 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7981 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     8041 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8101 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8161 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8221 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8281 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8341 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8401 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8461 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8521 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8581 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8641 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8701 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8761 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8821 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8881 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8941 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9001 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9061 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9121 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9181 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9241 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9301 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9421 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9481 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9601 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9661 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9841 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9901 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10021 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10081 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10141 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    10201 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    10261 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    10321 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    10381 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    10441 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    10501 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10561 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10621 cgttgtaaaa cgacggccag tgccaagctg
//
