LOCUS       V047095                 6997 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047095
VERSION     V047095
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..2047
                     /label="Acbd5(NM_001077635)"
                     /note="Acbd5(NM_001077635)"
                     /gene="Acbd5"
     CDS             2060..2125
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    2166..2390
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        2406..2929
                     /label="mCMV promoter"
     CDS             2943..3662
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3785..3906
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3913..4368)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4395..4499
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4501..4858
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4893..5687
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5919..5966
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6295..6883
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatggcg gacacacgat cagtgtatga
      661 aaccaggttt gaggcggccg tgaaggtgat ccaaagtttg ccgaaaaatg gatcattcca
      721 gccaacgaat gaaatgatgc tcaggttcta cagcttctac aagcaggcaa ctgaaggacc
      781 ctgtaaactc tcaaggcccg ggttctggga tcctattgga agatataaat gggatgcttg
      841 gagttccctg ggtgatatga ctaaagaaga agccatgatt gcatatgttg aagaaatgaa
      901 aaagattatt gaaactatgc ccatgactga gaaagtagaa gagttgctac atgttatagg
      961 tccattctat gagattgtag aagacaaaaa gaacagcaag agttctgact tgacctcaga
     1021 tcttggtaat gttctgacct cttcaaatgc caaagctgtt aatggtaaag cagagagcag
     1081 tgatagtggg gctgagtctg aggaagaaga ggcccaagaa gagttaaaag gagcagaaca
     1141 gagtggcagt gatgataaga aaatgatgac aaaatcaaca gataagaatt tggaaatcat
     1201 tgttaccaat ggctataaag atagctttgc tcaggacagt gacattcata ctgattcttc
     1261 ccggagtgcc agacgaagtg aagacaaaaa gcctacagac cagagctctc agcagacagg
     1321 aaacactgtc ctctgcgttc accaagatac aaacgaagac cccggtgaag acgcttcagg
     1381 agtacatcat ctgacaagtg actcagatag tgaagtatac tgtgattcta tggagcaatt
     1441 tggacaagag gagtattact taggtggtga tcctgctcag cacttggaag gttctggttt
     1501 ttgtgaagat gctcaactct ctcctgggaa tggcagcatt gggaaaatgc agatgagagc
     1561 agtcaaggga aaaggtgaag tgaagcacgg aggagaagat ggcagaagta gcagtggaac
     1621 gccgcaccgt gagaagagag gcggagagag tgaggacatc tccggtgtca ggagagggag
     1681 agtagggcat agaatgccac atttgagtga aggaaccaag ggtcggcaag taggaagtgg
     1741 aggtgatgga gaacgctggg gctcagaccg agggtccaga ggcagcctca atgagcagat
     1801 cgctcttgtg ctcattagat tgcaggagga catgcagaat gttcttcaga gacttcataa
     1861 actggaaaca ctaactgctt cgcaggcgaa attatcatgg cagacaagta atcagccctc
     1921 ctcacagaga ccatcttggt ggccctttga gatgtcccct ggtgcattag cttttgctat
     1981 tatatggcct tttattgctc agtggttggt gcatttgtat tatcaaagaa ggagaagaaa
     2041 actaaataag cttacgcgtg actacaaaga ccatgacggt gattataaag atcatgacat
     2101 cgactacaag gatgacgatg acaagtgagg atccgggccc gtttaaaccc gctgatcagc
     2161 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     2221 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     2281 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     2341 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg tctagagata
     2401 tactgagtca ttagggactt tccaatgggt tttgcccagt acataaggtc aataggggtg
     2461 aatcaacagg aaagtcccat tggagccaag tacactgagt caatagggac tttccattgg
     2521 gttttgccca gtacaaaagg tcaatagggg gtgagtcaat gggtttttcc cattattggc
     2581 acgtacataa ggtcaatagg ggtgagtcat tgggtttttc cagccaattt aattaaaacg
     2641 ccatgtactt tcccaccatt gacgtcaatg ggctattgaa actaatgcaa cgtgaccttt
     2701 aaacggtact ttcccatagc tgattaatgg gaaagtaccg ttctcgagcc aatacacgtc
     2761 aatgggaagt gaaagggcag ccaaaacgta acaccgcccc ggttttcccc tggaaattcc
     2821 atattggcac gcattctatt ggctgagctg cgttctacgt gggtataaga ggcgcgacca
     2881 gcgtcggtac cgtcgcagtc ttcggtctga ccaccgtaga acgcagatca ccggtcgcca
     2941 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3001 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3061 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3121 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3181 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3241 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3301 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3361 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3421 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3481 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3541 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     3601 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     3661 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3721 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3781 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3841 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3901 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     3961 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4021 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4081 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4141 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4201 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4261 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4321 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     4381 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4441 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     4501 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4561 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4681 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4741 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4801 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     4861 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4921 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4981 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5041 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     5101 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5161 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5221 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5281 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5341 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5401 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5461 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5521 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5581 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5641 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5701 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5761 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5821 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     5881 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     5941 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     6001 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     6061 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     6121 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     6181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6421 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     6481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6961 ccctgattct gtggataacc gtattaccgc catgcat
//
