LOCUS       V034624                 4754 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034624
VERSION     V034624
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             841..870
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             898..1872
                     /label="Arrdc5(NM_029799)"
                     /note="Arrdc5(NM_029799)"
                     /gene="Arrdc5"
     polyA_signal    1893..2027
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2138..2154)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2162..2178
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2186..2216)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2231..2252
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2540..3128)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3299..4159)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4160..4264)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4738..4754
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggcatcaatg
      841 gagcagaagc tgatctcaga ggaggacctg cttatggcca tggaggcccg aattcggatg
      901 tctgtggtga agtccatcga ggtagtgctg ccccaagatg cggtctacct ggccggctct
      961 atcatagacg ggcaggtggt cctcaccctc aacagtaccc tcgtggaccc cgtcgtgaag
     1021 gtggagcttg tcggtagggg ttacgttgag tggaacgaag aaattggaga gacccgtgat
     1081 tatagtagag atgttatttg caataataag gcagattacg tacataaaac aaaaacattc
     1141 ccaataaaag ataattggtt aagggcaggc agccacacct ttgacttcca tttcaactta
     1201 cctcccaggc tcccgtccac cttcaacagt aagatcggcc acatctccta ctttgtgcag
     1261 gccctgtgct tggaccggga gcatatcctg gccaagaaga agctgtacct gctggtccaa
     1321 gggatttctg agttccgcca gaggaatcta agtgagaact ccgtgtctgt agaagctgag
     1381 aagaaggtgt catacaactg ctgcagtcag ggctgggtga gcctgcatgt gcagatgagc
     1441 aagaacacct atgtgccggg cgagaaggtg acattcacat cagagatccg caatcacacg
     1501 ggcaagtaca tcaagaccgt ggtgttcgcc ctctatgccc atgtgcagta cgagggcttc
     1561 acgccaagtg ccgagaggcg ccggcgggca gacagcagcg agctgctgcg acagatggcc
     1621 aacgcacgga ttccagcctt caacagtacc acagtggtta gtgccttcaa cctgccgctg
     1681 gttttgtctg tgagcagtgg ctcccaggaa aatgagatca tgaggaccag ctatgagctt
     1741 gtagtcacga ttcacctccc ctggtctttg tcgacagtca aagcccgact ccccatcatc
     1801 atcaccagca ccagggaagg ccaggccgac tgcccccggc tggatgagct gccatatgag
     1861 gaccatatgg tttgaggtac cgcggccgcg gggatccaga catgataaga tacattgatg
     1921 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     1981 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     2041 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag
     2101 agtcgatctg caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2161 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2221 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2281 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2341 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2941 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3061 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3121 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3181 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3241 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3301 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3361 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3421 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3481 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3541 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3601 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3661 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3721 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3781 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3841 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3901 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3961 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4021 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4081 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4141 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4201 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4261 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     4321 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     4381 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     4441 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     4501 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     4561 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     4621 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     4681 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     4741 aaacgacggc cagt
//
