LOCUS       V044708                 5135 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044708
VERSION     V044708
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     intron          join(5054..5135,1..15)
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             79..105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             112..1167
                     /label="CCR5(NM_000579)"
                     /note="CCR5(NM_000579)"
                     /gene="CCR5"
     CDS             1255..1506
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    1521..1655
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1766..1782)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1790..1806
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1814..1844)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1859..1880
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2168..2756)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2927..3787)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3788..3892)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4366..4382
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        4409..4712
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        4713..4916
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
ORIGIN
        1 tgcctttact tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt
       61 acccgcgggc ccaccatgta cccatacgat gttccagatt acgctgaatt catggattat
      121 caagtgtcaa gtccaatcta tgacatcaat tattatacat cggagccctg ccaaaaaatc
      181 aatgtgaagc aaatcgcagc ccgcctcctg cctccgctct actcactggt gttcatcttt
      241 ggttttgtgg gcaacatgct ggtcatcctc atcctgataa actgcaaaag gctgaagagc
      301 atgactgaca tctacctgct caacctggcc atctctgacc tgtttttcct tcttactgtc
      361 cccttctggg ctcactatgc tgccgcccag tgggactttg gaaatacaat gtgtcaactc
      421 ttgacagggc tctattttat aggcttcttc tctggaatct tcttcatcat cctcctgaca
      481 atcgataggt acctggctgt cgtccatgct gtgtttgctt taaaagccag gacggtcacc
      541 tttggggtgg tgacaagtgt gatcacttgg gtggtggctg tgtttgcgtc tctcccagga
      601 atcatcttta ccagatctca aaaagaaggt cttcattaca cctgcagctc tcattttcca
      661 tacagtcagt atcaattctg gaagaatttc cagacattaa agatagtcat cttggggctg
      721 gtcctgccgc tgcttgtcat ggtcatctgc tactcgggaa tcctaaaaac tctgcttcgg
      781 tgtcgaaatg agaagaagag gcacagggct gtgaggctta tcttcaccat catgattgtt
      841 tattttctct tctgggctcc ctacaacatt gtccttctcc tgaacacctt ccaggaattc
      901 tttggcctga ataattgcag tagctctaac aggttggacc aagctatgca ggtgacagag
      961 actcttggga tgacgcactg ctgcatcaac cccatcatct atgcctttgt cggggagaag
     1021 ttcagaaact acctcttagt cttcttccaa aagcacattg ccaaacgctt ctgcaaatgc
     1081 tgttctattt tccagcaaga ggctcccgag cgagcaagct cagtttacac ccgatccact
     1141 ggggagcagg aaatatctgt gggcttgggt ggaggcggat caggaggcgg tggatctggt
     1201 acccgtccgg cgtgcaaaat cccgaacgac ctgaaacaga aagtcatgaa ccacgacaag
     1261 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     1321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1381 gacaaccact acctgagcta ccagtccaaa ctgagcaaag accccaacga gaagcgcgat
     1441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1501 tacaagtaag cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa
     1561 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     1621 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     1681 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca
     1741 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1801 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1861 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1921 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     1981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2581 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3061 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3901 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     3961 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4021 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4081 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4141 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     4201 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     4261 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4321 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4381 gtgagttcga gcttgcatgc ctgcaggtcg ttacataact tacggtaaat ggcccgcctg
     4441 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4501 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4561 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4621 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     4681 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     4741 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     4801 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     4861 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     4921 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     4981 accgggaccg atccagcctc cggactctag aggatccggt actagaggaa ctgaaaaacc
     5041 agaaagttaa ctggtaagtt tagtcttttt gtcttttatt tcaggtcccg gatccggtgg
     5101 tggtgcaaat caaagaactg ctcctcagtg gatgt
//
