LOCUS       V021823                 5801 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021823
VERSION     V021823
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             859..2892
                     /label="DHX58(NM_024119)"
                     /note="DHX58(NM_024119)"
                     /gene="DHX58"
     CDS             2902..2925
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    2940..3074
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3185..3201)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3209..3225
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3233..3263)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3278..3299
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3587..4175)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4346..5206)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5207..5311)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5785..5801
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt
      841 cgaccgagat ctgccaccat ggagcttcgg tcctaccaat gggaggtgat catgcctgcc
      901 ctggagggca agaatatcat catctggctg cccacgggtg ccgggaagac ccgggcggct
      961 gcttatgtgg ccaagcggca cctagagact gtggatggag ccaaggtggt tgtattggtc
     1021 aacagggtgc acctggtgac ccagcatggt gaagagttca ggcgcatgct ggatggacgc
     1081 tggaccgtga caaccctgag tggggacatg ggaccacgtg ctggctttgg ccacctggcc
     1141 cggtgccatg acctgctcat ctgcacagca gagcttctgc agatggcact gaccagcccc
     1201 gaggaggagg agcacgtgga gctcactgtc ttctccctga tcgtggtgga tgagtgccac
     1261 cacacgcaca aggacaccgt ctacaacgtc atcatgagcc agtacctaga acttaaactc
     1321 cagagggcac agccgctacc ccaggtgctg ggtctcacag cctccccagg cactggcggg
     1381 gcctccaaac tcgatggggc catcaaccac gtcctgcagc tctgtgccaa cttggacacg
     1441 tggtgcatca tgtcacccca gaactgctgc ccccagctgc aggagcacag ccaacagcct
     1501 tgcaaacagt acaacctctg ccacaggcgc agccaggatc cgtttgggga cttgctgaag
     1561 aagctcatgg accaaatcca tgaccacctg gagatgcctg agttgagccg gaaatttggg
     1621 acgcaaatgt atgagcagca ggtggtgaag ctgagtgagg ctgcggcttt ggctgggctt
     1681 caggagcaac gggtgtatgc gcttcacctg aggcgctaca atgacgcgct gctcatccat
     1741 gacaccgtcc gcgccgtgga tgccttggct gcgctgcagg atttctatca cagggagcac
     1801 gtcactaaaa cccagatcct gtgtgccgag cgccggctgc tggccctgtt cgatgaccgc
     1861 aagaatgagc tggcccactt ggcaactcat ggcccagaga atccaaaact ggagatgctg
     1921 gaaaagatcc tgcaaaggca gttcagtagc tctaacagcc ctcggggtat catcttcacc
     1981 cgcacccgcc aaagcgcaca ctccctcctg ctctggctcc agcagcagca gggcctgcag
     2041 actgtggaca tccgggccca gctactgatt ggggctggga acagcagcca gagcacccac
     2101 atgacccaga gggaccagca agaagtgatc cagaagttcc aagatggaac cctgaacctt
     2161 ctggtggcca cgagtgtggc ggaggagggg ctggacatcc cacattgcaa tgtggtggtg
     2221 cgttatgggc tcttgaccaa tgaaatctcc atggtccagg ccaggggccg tgcccgggcc
     2281 gatcagagtg tatacgcgtt tgtagcaact gaaggtagcc gggagctgaa gcgggagctg
     2341 atcaacgagg cgctggagac gctgatggag caggcagtgg ctgctgtgca gaaaatggac
     2401 caggccgagt accaggccaa gatccgggat ctgcagcagg cagccttgac caagcgggcg
     2461 gcccaggcag cccagcggga gaaccagcgg cagcagttcc cagtggagca cgtgcagcta
     2521 ctctgcatca actgcatggt ggctgtgggc catggcagcg acctgcggaa ggtggagggc
     2581 acccaccatg tcaatgtgaa ccccaacttc tcgaactact ataatgtctc cagggatcct
     2641 gtggtcatca acaaagtctt caaggactgg aagcctgggg gtgtcatcag ctgcaggaac
     2701 tgtggggagg tctggggtct gcagatgatc tacaagtcag tgaagctgcc agtgctcaaa
     2761 gtccgcagca tgctgctgga gacccctcag gggcggatcc aggccaaaaa gtggtcccgc
     2821 gtgcccttct ccgtgcctga ctttgacttc ctgcagcatt gtgccgagaa cttgtcggac
     2881 ctctccctgg acggtaccgg cgactacaag gacgacgatg acaagtaagc ggccgcgggg
     2941 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3001 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3061 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     3121 tgtgggaggt tttttcggat cctctagagt cgatctgcag gcatgctagc ttggcgtaat
     3181 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3241 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3301 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3361 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3421 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3481 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3541 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4021 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     5401 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     5461 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     5521 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     5581 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     5641 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     5701 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     5761 cagggttttc ccagtcacga cgttgtaaaa cgacggccag t
//
