LOCUS V045062 7455 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045062 VERSION V045062 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 928..954 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 958..984 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 988..1014 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1021..3486 /label="FGFR1(NM_023110)" /note="FGFR1(NM_023110)" /gene="FGFR1" polyA_signal 3520..3744 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3790..4218 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4232..4561 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4573..4971 /codon_start=1 /gene="Aspergillus terreus BSD" /note="confers resistance to blasticidin" /product="blasticidin S deaminase" /transl_table=1 /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG*" /label="BSD" polyA_signal 5131..5252 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5301..5317) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5325..5341 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5349..5379) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5394..5415 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5703..6288) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6459..7319) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7320..7424) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 aagctttcta gagaattcgc caccatgtac ccatacgacg tacctgatta tgcaacttat 961 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcactcgag 1021 atgtggagct ggaagtgcct cctcttctgg gctgtgctgg tcacagccac actctgcacc 1081 gctaggccgt ccccgacctt gcctgaacaa gcccagccct ggggagcccc tgtggaagtg 1141 gagtccttcc tggtccaccc cggtgacctg ctgcagcttc gctgtcggct gcgggacgat 1201 gtgcagagca tcaactggct gcgggacggg gtgcagctgg cggaaagcaa ccgcacccgc 1261 atcacagggg aggaggtgga ggtgcaggac tccgtgcccg cagactccgg cctctatgct 1321 tgcgtaacca gcagcccctc gggcagtgac accacctact tctccgtcaa tgtttcagat 1381 gctctcccct cctcggagga tgatgatgat gatgatgact cctcttcaga ggagaaagaa 1441 acagataaca ccaaaccaaa ccgtatgccc gtagctccat attggacatc cccagaaaag 1501 atggaaaaga aattgcatgc agtgccggct gccaagacag tgaagttcaa atgcccttcc 1561 agtgggaccc caaaccccac actgcgctgg ttgaaaaatg gcaaagaatt caaacctgac 1621 cacagaattg gaggctacaa ggtccgttat gccacctgga gcatcataat ggactctgtg 1681 gtgccctctg acaagggcaa ctacacctgc attgtggaga atgagtacgg cagcatcaac 1741 cacacatacc agctggatgt cgtggagcgg tcccctcacc ggcccatcct gcaagcaggg 1801 ttgcccgcca acaaaacagt ggccctgggt agcaacgtgg agttcatgtg taaggtgtac 1861 agtgacccgc agccgcacat ccagtggcta aagcacatcg aggtgaatgg gagcaagatt 1921 ggcccagaca acctgcctta tgtccagatc ttgaagactg ctggagttaa taccaccgac 1981 aaagagatgg aggtgcttca cttaagaaat gtctcctttg aggacgcagg ggagtatacg 2041 tgcttggcgg gtaactctat cggactctcc catcactctg catggttgac cgttctggaa 2101 gccctggaag agaggccggc agtgatgacc tcgcccctgt acctggagat catcatctat 2161 tgcacagggg ccttcctcat ctcctgcatg gtggggtcgg tcatcgtcta caagatgaag 2221 agtggtacca agaagagtga cttccacagc cagatggctg tgcacaagct ggccaagagc 2281 atccctctgc gcagacaggt aacagtgtct gctgactcca gtgcatccat gaactctggg 2341 gttcttctgg ttcggccatc acggctctcc tccagtggga ctcccatgct agcaggggtc 2401 tctgagtatg agcttcccga agaccctcgc tgggagctgc ctcgggacag actggtctta 2461 ggcaaacccc tgggagaggg ctgctttggg caggtggtgt tggcagaggc tatcgggctg 2521 gacaaggaca aacccaaccg tgtgaccaaa gtggctgtga agatgttgaa gtcggacgca 2581 acagagaaag acttgtcaga cctgatctca gaaatggaga tgatgaagat gatcgggaag 2641 cataagaata tcatcaacct gctgggggcc tgcacgcagg atggtccctt gtatgtcatc 2701 gtggagtatg cctccaaggg caacctgcgg gagtacctgc aggcccggag gcccccaggg 2761 ctggaatact gctacaaccc cagccacaac ccagaggagc agctctcctc caaggacctg 2821 gtgtcctgcg cctaccaggt ggcccgaggc atggagtatc tggcctccaa gaagtgcata 2881 caccgagacc tggcagccag gaatgtcctg gtgacagagg acaatgtgat gaagatagca 2941 gactttggcc tcgcacggga cattcaccac atcgactact ataaaaagac aaccaacggc 3001 cgactgcctg tgaagtggat ggcacccgag gcattatttg accggatcta cacccaccag 3061 agtgatgtgt ggtctttcgg ggtgctcctg tgggagatct tcactctggg cggctcccca 3121 taccccggtg tgcctgtgga ggaacttttc aagctgctga aggagggtca ccgcatggac 3181 aagcccagta actgcaccaa cgagctgtac atgatgatgc gggactgctg gcatgcagtg 3241 ccctcacaga gacccacctt caagcagctg gtggaagacc tggaccgcat cgtggccttg 3301 acctccaacc aggagtacct ggacctgtcc atgcccctgg accagtactc ccccagcttt 3361 cccgacaccc ggagctctac gtgctcctca ggggaggatt ccgtcttctc tcatgagccg 3421 ctgcccgagg agccctgcct gccccgacac ccagcccagc ttgccaatgg cggactcaaa 3481 cgccgctagg atccagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc 3541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3661 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 3721 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 3781 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 3841 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 3901 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 3961 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 4021 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 4081 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 4141 ttgatttata agggattttg gccatttcgg cctattggtt aaaaaatgag ctgatttaac 4201 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 4261 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 4321 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 4381 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 4441 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 4501 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 4561 aaagctcccg ggatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca 4621 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc 4681 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt 4741 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc 4801 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg 4861 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg 4921 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta attcgaaatg 4981 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 5041 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 5101 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 5161 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 5221 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 5281 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 5341 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 5401 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 5461 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 5521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 5581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 5641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 5701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 6181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 6361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 6421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 6481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 6541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 6601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 6661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 6721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 6781 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 6841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 6901 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 6961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 7021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 7081 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 7141 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 7201 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 7261 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 7321 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 7381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 7441 gtgccacctg acgtc //