LOCUS       V045062                 7455 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045062
VERSION     V045062
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             928..954
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             958..984
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             988..1014
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1021..3486
                     /label="FGFR1(NM_023110)"
                     /note="FGFR1(NM_023110)"
                     /gene="FGFR1"
     polyA_signal    3520..3744
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3790..4218
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4232..4561
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4573..4971
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG*"
                     /label="BSD"
     polyA_signal    5131..5252
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5301..5317)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5325..5341
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5349..5379)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5394..5415
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5703..6288)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6459..7319)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7320..7424)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcgc caccatgtac ccatacgacg tacctgatta tgcaacttat
      961 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcactcgag
     1021 atgtggagct ggaagtgcct cctcttctgg gctgtgctgg tcacagccac actctgcacc
     1081 gctaggccgt ccccgacctt gcctgaacaa gcccagccct ggggagcccc tgtggaagtg
     1141 gagtccttcc tggtccaccc cggtgacctg ctgcagcttc gctgtcggct gcgggacgat
     1201 gtgcagagca tcaactggct gcgggacggg gtgcagctgg cggaaagcaa ccgcacccgc
     1261 atcacagggg aggaggtgga ggtgcaggac tccgtgcccg cagactccgg cctctatgct
     1321 tgcgtaacca gcagcccctc gggcagtgac accacctact tctccgtcaa tgtttcagat
     1381 gctctcccct cctcggagga tgatgatgat gatgatgact cctcttcaga ggagaaagaa
     1441 acagataaca ccaaaccaaa ccgtatgccc gtagctccat attggacatc cccagaaaag
     1501 atggaaaaga aattgcatgc agtgccggct gccaagacag tgaagttcaa atgcccttcc
     1561 agtgggaccc caaaccccac actgcgctgg ttgaaaaatg gcaaagaatt caaacctgac
     1621 cacagaattg gaggctacaa ggtccgttat gccacctgga gcatcataat ggactctgtg
     1681 gtgccctctg acaagggcaa ctacacctgc attgtggaga atgagtacgg cagcatcaac
     1741 cacacatacc agctggatgt cgtggagcgg tcccctcacc ggcccatcct gcaagcaggg
     1801 ttgcccgcca acaaaacagt ggccctgggt agcaacgtgg agttcatgtg taaggtgtac
     1861 agtgacccgc agccgcacat ccagtggcta aagcacatcg aggtgaatgg gagcaagatt
     1921 ggcccagaca acctgcctta tgtccagatc ttgaagactg ctggagttaa taccaccgac
     1981 aaagagatgg aggtgcttca cttaagaaat gtctcctttg aggacgcagg ggagtatacg
     2041 tgcttggcgg gtaactctat cggactctcc catcactctg catggttgac cgttctggaa
     2101 gccctggaag agaggccggc agtgatgacc tcgcccctgt acctggagat catcatctat
     2161 tgcacagggg ccttcctcat ctcctgcatg gtggggtcgg tcatcgtcta caagatgaag
     2221 agtggtacca agaagagtga cttccacagc cagatggctg tgcacaagct ggccaagagc
     2281 atccctctgc gcagacaggt aacagtgtct gctgactcca gtgcatccat gaactctggg
     2341 gttcttctgg ttcggccatc acggctctcc tccagtggga ctcccatgct agcaggggtc
     2401 tctgagtatg agcttcccga agaccctcgc tgggagctgc ctcgggacag actggtctta
     2461 ggcaaacccc tgggagaggg ctgctttggg caggtggtgt tggcagaggc tatcgggctg
     2521 gacaaggaca aacccaaccg tgtgaccaaa gtggctgtga agatgttgaa gtcggacgca
     2581 acagagaaag acttgtcaga cctgatctca gaaatggaga tgatgaagat gatcgggaag
     2641 cataagaata tcatcaacct gctgggggcc tgcacgcagg atggtccctt gtatgtcatc
     2701 gtggagtatg cctccaaggg caacctgcgg gagtacctgc aggcccggag gcccccaggg
     2761 ctggaatact gctacaaccc cagccacaac ccagaggagc agctctcctc caaggacctg
     2821 gtgtcctgcg cctaccaggt ggcccgaggc atggagtatc tggcctccaa gaagtgcata
     2881 caccgagacc tggcagccag gaatgtcctg gtgacagagg acaatgtgat gaagatagca
     2941 gactttggcc tcgcacggga cattcaccac atcgactact ataaaaagac aaccaacggc
     3001 cgactgcctg tgaagtggat ggcacccgag gcattatttg accggatcta cacccaccag
     3061 agtgatgtgt ggtctttcgg ggtgctcctg tgggagatct tcactctggg cggctcccca
     3121 taccccggtg tgcctgtgga ggaacttttc aagctgctga aggagggtca ccgcatggac
     3181 aagcccagta actgcaccaa cgagctgtac atgatgatgc gggactgctg gcatgcagtg
     3241 ccctcacaga gacccacctt caagcagctg gtggaagacc tggaccgcat cgtggccttg
     3301 acctccaacc aggagtacct ggacctgtcc atgcccctgg accagtactc ccccagcttt
     3361 cccgacaccc ggagctctac gtgctcctca ggggaggatt ccgtcttctc tcatgagccg
     3421 ctgcccgagg agccctgcct gccccgacac ccagcccagc ttgccaatgg cggactcaaa
     3481 cgccgctagg atccagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc
     3541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3661 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3721 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3781 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3841 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3901 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3961 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4021 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4081 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4141 ttgatttata agggattttg gccatttcgg cctattggtt aaaaaatgag ctgatttaac
     4201 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4261 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4321 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4381 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4441 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4501 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4561 aaagctcccg ggatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     4621 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     4681 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     4741 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     4801 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     4861 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     4921 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta attcgaaatg
     4981 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5041 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5101 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     5161 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5221 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     5281 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5341 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5401 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5461 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6781 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6901 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7081 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7141 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7201 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7261 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7321 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7441 gtgccacctg acgtc
//
