LOCUS 40924_11665 5860 bp DNA circular SYN 01-JAN-1980 DEFINITION Control vector for constitutive high-level expression of intracellular green Renilla luciferase under control of the CMV promoter. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5860) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 5860) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5860 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 63..366 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 367..570 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 615..633 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 646..1578 /codon_start=1 /label=Green Renilla Luciferase /note="green-emitting variant of Renilla luciferase" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ" polyA_signal 1604..1715 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 1838..2167 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 2215..2262 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 2281..2877 /codon_start=1 /label=PuroR /note="puromycin N-acetyltransferase" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" polyA_signal 3010..3143 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(3187..4044) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4308..4896 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5166..5182 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 5638..5654 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 5769..5860 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 541 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac 601 tggcttatcg aaattaatac gactcactat aggggatccg ccaccatggc cagcaaggtg 661 tacgaccccg agcagcggaa gcggatgatc accggccctc agtggtgggc tcggtgcaag 721 cagatgaacg tgctggacag cttcatcaac tactacgaca gcgagaagca cgccgagaac 781 gccgtgatct tcctgcacgg caacgccacc agcagctacc tgtggcggca cgtggtgccc 841 cacatcgagc ctgtggccag atgcatcatc cccgacctga tcggcatggg caagagcggc 901 aagtccggca acggcagcta ccggctgctg gaccactaca agtacctgac cgcttggttt 961 gagctgctga acctgcccaa gaagatcatc ttcgtcggcc acgactgggg cagcgccctg 1021 gcctttcact acgcctacga gcaccaggac cggatcaagg ccatcgtgca catggaaagc 1081 gtggtggacg tgatcgagag ctggatgggc tggcccgaca tcgaggaaga actggccctg 1141 atcaagagcg aagagggcga gaagatggtg ctggaaaaca acttcttcgt ggaaaccctg 1201 ctgcccagca agatcatgcg gaagctggaa cccgaagagt tcgccgccta cctggaaccc 1261 ttcaaagaaa agggcgaagt gcggaggccc accctgagct ggcccagaga gatccccctg 1321 gtcaagggcg gcaagcccga cgtggtgcag atcgtgcgga actacaacgc ctacctgcgg 1381 gccagcgacg acctgcctaa gctgttcatc gagagcgacc ccggcttctt cagcaacgcc 1441 atcgtggaag gcgccaagaa gttccccaac accgagttcg tgaaagtgaa gggcctgcac 1501 ttcctccagg aagatgcccc cgacgagatg ggcaagtaca tcaagagctt cgtggaacgg 1561 gtgctgaaga acgagcagtg agcggccgca aaatcagcct cgactgtgcc ttctagttgc 1621 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 1681 actgtccttt cctaataaaa tgaggaaatt gcatcacaac actcaaccct atctcggtct 1741 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 1801 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa 1861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 1921 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 1981 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 2041 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 2101 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 2161 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac 2221 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc 2281 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 2341 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 2401 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 2461 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 2521 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 2581 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 2641 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 2701 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 2761 gagacatccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 2821 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 2881 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 2941 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 3001 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 3061 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 3121 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 3181 tcattaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 3241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 3301 ccccagcgct gcgatgatac cgcgagaacc acgctcaccg gctccggatt tatcagcaat 3361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 3421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 3481 caacgttgtt gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 3541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 3601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 3661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 3721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 3781 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 3841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 3901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 3961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 4021 gacacggaaa tgttgaatac tcatattctt cctttttcaa tattattgaa gcatttatca 4081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4141 ggtcagtgtt acaaccaatt aaccaattct gaacattatc gcgagcccat ttatacctga 4201 atatggctca taacacccct tgctcatgac caaaatccct taacgtgagt tacgcgcgcg 4261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4321 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4381 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4441 ataccaaata ctgttcttct agtgtagccg tagttagccc accacttcaa gaactctgta 4501 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4561 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4621 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4681 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4741 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4801 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4861 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4921 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4981 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 5041 accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga actgccaggc 5101 atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca aactctttct 5161 gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct gataacgagt 5221 aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt 5281 ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct tgatatatga 5341 gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt 5401 tttcgattga tgaacaccta taattaaact attcatctat tatttatgat tttttgtata 5461 tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa taataaaggg 5521 aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat ataatacaaa 5581 ctataagatg ttatcagtat ttattatcat ttagaataaa ttttgtgtcg cccttaattg 5641 tgagcggata acaattacga gcttcatgca cagtggcgtt gacattgatt attgactagc 5701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag 5761 ttattaataa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag 5821 gctgtcccca gtgcaagtgc aggtgccaga acatttctct //