LOCUS       V019642                 5644 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019642
VERSION     V019642
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..855
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             859..2757
                     /label="Hspa2(NM_001002012)"
                     /note="Hspa2(NM_001002012)"
                     /gene="Hspa2"
     polyA_signal    2783..2917
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3028..3044)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3052..3068
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3076..3106)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3121..3142
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3430..4018)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4189..5049)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5050..5154)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5628..5644
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag
      841 gacgacgatg acaagcttat gtctgcccgc ggcccggcta tcggcatcga cctgggcacc
      901 acttactcgt gcgtgggggt attccaacat ggcaaggtgg agatcatcgc caacgaccag
      961 ggtaaccgca ccacccccag ctacgtggcc ttcactgaca ccgagcgtct catcggcgac
     1021 gccgccaaga accaagtggc catgaacccc acaaacacca tcttcgacgc caagcggctg
     1081 atcggacgga agttcgagga cgccacagtg cagtccgata tgaagcactg gccgttccga
     1141 gtggtgagcg aaggtgggaa gcccaaagtg caggtggaat ataaagggga gatgaagacc
     1201 ttcttccctg aggagatttc ttccatggtc ctcactaaga tgaaggagat cgccgaagcc
     1261 tacctggggg gcaaggtgca gagcgcagtc atcactgttc ctgcctattt caacgactcg
     1321 cagcgccagg ccaccaagga tgcgggcacc atcaccggcc tcaacgtgtt gcgcatcatc
     1381 aatgagccca cagcagcggc catcgcctac ggcctggata agaagggctg tgcgggcggc
     1441 gagaagaacg tgctcatctt tgacctgggc gggggcacct tcgatgtgtc catcctgacc
     1501 atcgaggatg gcatctttga ggtgaagtcc acggccggcg atacccacct gggtggcgaa
     1561 gacttcgaca accgtatggt cagccacctg gcggaggagt tcaagcgcaa acacaagaag
     1621 gacattgggc ccaacaagcg cgctgtgcgc cggctgcgca ccgcctgcga gcgcgctaag
     1681 cgcaccctga gctcgtccac gcaggccagc atagagatcg actcgctcta cgagggcgtg
     1741 gatttctaca cgtccatcac ccgcgcccgc ttcgaggagc tcaacgccga tctcttccga
     1801 gggaccctgg agccggtgga aaaggcgctg cgcgatgcca agctagacaa gggccagatc
     1861 caggagatag tgctggtggg cggctcaacc cgcatcccta agatccagaa gctcctgcaa
     1921 gatttcttca acggcaagga gctgaacaag agcattaatc ccgacgaggc ggtggcctac
     1981 ggcgccgctg tgcaggcggc tatcctcatc ggcgacaagt cggaaaatgt gcaggatctg
     2041 ctgttactcg acgtgactcc attgtcgctc ggcatcgaaa cagctggcgg tgtcatgacc
     2101 ccactcatca agagaaacac cacgatcccc accaagcaga cgcagacctt cactacctac
     2161 tcagacaacc agagcagcgt gctggtgcaa gtgtacgagg gcgaacgggc catgaccaag
     2221 gacaataacc tcttgggcaa gttcgacctg actgggatcc ccccagcacc ccgtggggtc
     2281 ccccagatcg aggtcacctt tgacatcgat gccaacggca tccttaacgt cactgctgcc
     2341 gacaagagca ccggtaaaga aaataaaatc accataacca acgacaaggg tcggctgagc
     2401 aaagacgaca ttgaccggat ggtgcaggag gcggagcggt acaaatcgga agatgaagca
     2461 aatcgcgatc gcgtggcagc caaaaatgcg gtggagtcct atacctacaa catcaagcag
     2521 accgtggaag acgagaaact gaggggcaag attagcgagc aggacaaaaa caagatcctc
     2581 gacaagtgtc aggaggtgat caactggctt gaccgaaacc agatggcaga gaaagatgag
     2641 tacgaacaca agcagaaaga gcttgagaga gtgtgcaacc ccatcatcag caaactttac
     2701 caaggcggtc caggcggcgg cggctcctct ggagggccca ccatcgagga agtggactag
     2761 ctcgaggtac cgcggccgcg gggatccaga catgataaga tacattgatg agtttggaca
     2821 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2881 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2941 tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag agtcgatctg
     3001 caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3061 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3241 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3841 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4081 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4141 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4201 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4261 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4321 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4381 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4441 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4501 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4561 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4621 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4681 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4741 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4801 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4861 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4921 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4981 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5041 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5101 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5161 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     5221 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     5281 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     5341 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     5401 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     5461 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     5521 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     5581 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     5641 cagt
//
