LOCUS V019642 5644 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019642 VERSION V019642 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 27..330 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 331..534 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 672..768 /note="modified SV40 intron with splice donor and acceptor sites" /label="SV40 intron" CDS 832..855 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 859..2757 /label="Hspa2(NM_001002012)" /note="Hspa2(NM_001002012)" /gene="Hspa2" polyA_signal 2783..2917 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3028..3044) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3052..3068 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3076..3106) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3121..3142 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(3430..4018) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4189..5049) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5050..5154) /gene="bla" /label="AmpR promoter" primer_bind 5628..5644 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc 61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta 541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac 601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag 661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg 721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa 781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag 841 gacgacgatg acaagcttat gtctgcccgc ggcccggcta tcggcatcga cctgggcacc 901 acttactcgt gcgtgggggt attccaacat ggcaaggtgg agatcatcgc caacgaccag 961 ggtaaccgca ccacccccag ctacgtggcc ttcactgaca ccgagcgtct catcggcgac 1021 gccgccaaga accaagtggc catgaacccc acaaacacca tcttcgacgc caagcggctg 1081 atcggacgga agttcgagga cgccacagtg cagtccgata tgaagcactg gccgttccga 1141 gtggtgagcg aaggtgggaa gcccaaagtg caggtggaat ataaagggga gatgaagacc 1201 ttcttccctg aggagatttc ttccatggtc ctcactaaga tgaaggagat cgccgaagcc 1261 tacctggggg gcaaggtgca gagcgcagtc atcactgttc ctgcctattt caacgactcg 1321 cagcgccagg ccaccaagga tgcgggcacc atcaccggcc tcaacgtgtt gcgcatcatc 1381 aatgagccca cagcagcggc catcgcctac ggcctggata agaagggctg tgcgggcggc 1441 gagaagaacg tgctcatctt tgacctgggc gggggcacct tcgatgtgtc catcctgacc 1501 atcgaggatg gcatctttga ggtgaagtcc acggccggcg atacccacct gggtggcgaa 1561 gacttcgaca accgtatggt cagccacctg gcggaggagt tcaagcgcaa acacaagaag 1621 gacattgggc ccaacaagcg cgctgtgcgc cggctgcgca ccgcctgcga gcgcgctaag 1681 cgcaccctga gctcgtccac gcaggccagc atagagatcg actcgctcta cgagggcgtg 1741 gatttctaca cgtccatcac ccgcgcccgc ttcgaggagc tcaacgccga tctcttccga 1801 gggaccctgg agccggtgga aaaggcgctg cgcgatgcca agctagacaa gggccagatc 1861 caggagatag tgctggtggg cggctcaacc cgcatcccta agatccagaa gctcctgcaa 1921 gatttcttca acggcaagga gctgaacaag agcattaatc ccgacgaggc ggtggcctac 1981 ggcgccgctg tgcaggcggc tatcctcatc ggcgacaagt cggaaaatgt gcaggatctg 2041 ctgttactcg acgtgactcc attgtcgctc ggcatcgaaa cagctggcgg tgtcatgacc 2101 ccactcatca agagaaacac cacgatcccc accaagcaga cgcagacctt cactacctac 2161 tcagacaacc agagcagcgt gctggtgcaa gtgtacgagg gcgaacgggc catgaccaag 2221 gacaataacc tcttgggcaa gttcgacctg actgggatcc ccccagcacc ccgtggggtc 2281 ccccagatcg aggtcacctt tgacatcgat gccaacggca tccttaacgt cactgctgcc 2341 gacaagagca ccggtaaaga aaataaaatc accataacca acgacaaggg tcggctgagc 2401 aaagacgaca ttgaccggat ggtgcaggag gcggagcggt acaaatcgga agatgaagca 2461 aatcgcgatc gcgtggcagc caaaaatgcg gtggagtcct atacctacaa catcaagcag 2521 accgtggaag acgagaaact gaggggcaag attagcgagc aggacaaaaa caagatcctc 2581 gacaagtgtc aggaggtgat caactggctt gaccgaaacc agatggcaga gaaagatgag 2641 tacgaacaca agcagaaaga gcttgagaga gtgtgcaacc ccatcatcag caaactttac 2701 caaggcggtc caggcggcgg cggctcctct ggagggccca ccatcgagga agtggactag 2761 ctcgaggtac cgcggccgcg gggatccaga catgataaga tacattgatg agtttggaca 2821 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc 2881 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt 2941 tatgtttcag gttcaggggg aggtgtggga ggttttttcg gatcctctag agtcgatctg 3001 caggcatgct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3061 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 3121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3241 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3301 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3361 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3421 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3481 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3541 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3601 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 3661 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3721 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3781 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 3841 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 3901 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 3961 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4021 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4081 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4141 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4201 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4261 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4321 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4381 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4441 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4501 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4561 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4621 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4681 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4741 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4801 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4861 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 4921 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 4981 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5041 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5101 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5161 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5221 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 5281 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 5341 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 5401 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 5461 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 5521 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 5581 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 5641 cagt //