LOCUS       V026285                 6920 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026285
VERSION     V026285
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..4011
                     /label="KIF11(NM_004523)"
                     /note="KIF11(NM_004523)"
                     /gene="KIF11"
     CDS             4021..4044
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    4059..4193
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4304..4320)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4328..4344
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4352..4382)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4397..4418
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(4706..5294)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5465..6325)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6326..6430)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     6904..6920
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatggcgt cgcagccaaa ttcgtctgcg aagaagaaag aggagaaggg gaagaacatc
      901 caggtggtgg tgagatgcag accatttaat ttggcagagc ggaaagctag cgcccattca
      961 atagtagaat gtgatcctgt acgaaaagaa gttagtgtac gaactggagg attggctgac
     1021 aagagctcaa ggaaaacata cacttttgat atggtgtttg gagcatctac taaacagatt
     1081 gatgtttacc gaagtgttgt ttgtccaatt ctggatgaag ttattatggg ctataattgc
     1141 actatctttg cgtatggcca aactggcact ggaaaaactt ttacaatgga aggtgaaagg
     1201 tcacctaatg aagagtatac ctgggaagag gatcccttgg ctggtataat tccacgtacc
     1261 cttcatcaaa tttttgagaa acttactgat aatggtactg aattttcagt caaagtgtct
     1321 ctgttggaga tctataatga agagcttttt gatcttctta atccatcatc tgatgtttct
     1381 gagagactac agatgtttga tgatccccgt aacaagagag gagtgataat taaaggttta
     1441 gaagaaatta cagtacacaa caaggatgaa gtctatcaaa ttttagaaaa gggggcagca
     1501 aaaaggacaa ctgcagctac tctgatgaat gcatactcta gtcgttccca ctcagttttc
     1561 tctgttacaa tacatatgaa agaaactacg attgatggag aagagcttgt taaaatcgga
     1621 aagttgaact tggttgatct tgcaggaagt gaaaacattg gccgttctgg agctgttgat
     1681 aagagagctc gggaagctgg aaatataaat caatccctgt tgactttggg aagggtcatt
     1741 actgcccttg tagaaagaac acctcatgtt ccttatcgag aatctaaact aactagaatc
     1801 ctccaggatt ctcttggagg gcgtacaaga acatctataa ttgcaacaat ttctcctgca
     1861 tctctcaatc ttgaggaaac tctgagtaca ttggaatatg ctcatagagc aaagaacata
     1921 ttgaataagc ctgaagtgaa tcagaaactc accaaaaaag ctcttattaa ggagtatacg
     1981 gaggagatag aacgtttaaa acgagatctt gctgcagccc gtgagaaaaa tggagtgtat
     2041 atttctgaag aaaattttag agtcatgagt ggaaaattaa ctgttcaaga agagcagatt
     2101 gtagaattga ttgaaaaaat tggtgctgtt gaggaggagc tgaatagggt tacagagttg
     2161 tttatggata ataaaaatga acttgaccag tgtaaatctg acctgcaaaa taaaacacaa
     2221 gaacttgaaa ccactcaaaa acatttgcaa gaaactaaat tacaacttgt taaagaagaa
     2281 tatatcacat cagctttgga aagtactgag gagaaacttc atgatgctgc cagcaagctg
     2341 cttaacacag ttgaagaaac tacaaaagat gtatctggtc tccattccaa actggatcgt
     2401 aagaaggcag ttgaccaaca caatgcagaa gctcaggata tttttggcaa aaacctgaat
     2461 agtctgttta ataatatgga agaattaatt aaggatggca gctcaaagca aaaggccatg
     2521 ctagaagtac ataagacctt atttggtaat ctgctgtctt ccagtgtctc tgcattagat
     2581 accattacta cagtagcact tggatctctc acatctattc cagaaaatgt gtctactcat
     2641 gtttctcaga tttttaatat gatactaaaa gaacaatcat tagcagcaga aagtaaaact
     2701 gtactacagg aattgattaa tgtactcaag actgatcttc taagttcact ggaaatgatt
     2761 ttatccccaa ctgtggtgtc tatactgaaa atcaatagtc aactaaagca tattttcaag
     2821 acttcattga cagtggccga taagatagaa gatcaaaaaa aggaactaga tggctttctc
     2881 agtatactgt gtaacaatct acatgaacta caagaaaata ccatttgttc cttggttgag
     2941 tcacaaaagc aatgtggaaa cctaactgaa gacctgaaga caataaagca gacccattcc
     3001 caggaacttt gcaagttaat gaatctttgg acagagagat tctgtgcttt ggaggaaaag
     3061 tgtgaaaata tacagaaacc acttagtagt gtccaggaaa atatacagca gaaatctaag
     3121 gatatagtca acaaaatgac ttttcacagt caaaaatttt gtgctgattc tgatggcttc
     3181 tcacaggaac tcagaaattt taaccaagaa ggtacaaaat tggttgaaga atctgtgaaa
     3241 cactctgata aactcaatgg caacctggaa aaaatatctc aagagactga acagagatgt
     3301 gaatctctga acacaagaac agtttatttt tctgaacagt gggtatcttc cttaaatgaa
     3361 agggaacagg aacttcacaa cttattggag gttgtaagcc aatgttgtga ggcttcaagt
     3421 tcagacatca ctgagaaatc agatggacgt aaggcagctc atgagaaaca gcataacatt
     3481 tttcttgatc agatgactat tgatgaagat aaattgatag cacaaaatct agaacttaat
     3541 gaaaccataa aaattggttt gactaagctt aattgctttc tggaacagga tctgaaactg
     3601 gatatcccaa caggtacgac accacagagg aaaagttatt tatacccatc aacactggta
     3661 agaactgaac cacgtgaaca tctccttgat cagctgaaaa ggaaacagcc tgagctgtta
     3721 atgatgctaa actgttcaga aaacaacaaa gaagagacaa ttccggatgt ggatgtagaa
     3781 gaggcagttc tggggcagta tactgaagaa cctctaagtc aagagccatc tgtagatgct
     3841 ggtgtggatt gttcatcaat tggcggggtt ccatttttcc agcataaaaa atcacatgga
     3901 aaagacaaag aaaacagagg cattaacaca ctggagaggt ctaaagtgga agaaactaca
     3961 gagcacttgg ttacaaagag cagattacct ctgcgagccc agatcaacct tggtaccggc
     4021 gactacaagg acgacgatga caagtaagcg gccgcgggga tccagacatg ataagataca
     4081 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4141 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4201 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttcggatc
     4261 ctctagagtc gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg
     4321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4381 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     5161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     6481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     6541 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6661 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     6721 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     6781 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6841 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6901 gttgtaaaac gacggccagt
//
